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Neuroimaging studies connecting neurocysticercosis along with mesial temporary lobe epilepsy with hippocampal sclerosis.
The presented study is focused on the development of electrochemical genosensor for detection of tox gene fragment of toxigenic Corynebacterium diphtheriae strain. Together with our previous studies it fulfils the whole procedure for fast and accurate diagnostic of diphtheria at its early stage of infection with the use of electrochemical methods. The developed DNA sensor potentially can be used in more sophisticated portable device. After the electrochemical stem-loop probe structure optimization the conditions for real asymmetric PCR (aPCR) product detection were selected. As was shown it was crucial to optimize the magnesium and organic solvent concentrations in detection buffer. Under optimal conditions it was possible to selectively detect as low as 20.8 nM of complementary stand in 5 min or 0.5 nM in 30 min with sensitivity of 12.81 and 0.24 1⋅μM-1 respectively. The unspecific biosensor response was elucidated with the use of new electrode blocking agent, diethyldithiocarbamate. Its application in electrochemical genosensors lead to significant higher current values and the biosensor response even in conditions with magnesium ion depletion. The developed biosensor selectivity was examined using samples containing genetic material originated from a number of non-target bacterial species which potentially can be present in the human upper respiratory tract.Seven artworks representing the diversity of paints used around the 1960s and created by German and Italian painters (J. Albers, A. Bonalumi, L. Boille, T. Scialoja and M. Schifano) were studied on-site at the Galleria Nazionale d'Arte Moderna (Rome) with mobile instruments. We present a methodology based on Specular Reflectance Infrared Spectroscopy (SR-FTIR) adapted to unvarnished paintings. Complementary measurements have been performed by Raman spectroscopy. Characteristic bands regarding as-recorded infrared reflectance spectra and Kramers-Kronig Transformation-converted absorbance spectra are identified according to literature and reference spectra recorded on representative commercially available paints. To distinguish the different binders by SR-FTIR, we propose spectroscopic markers as the comparison of the intensity of carbonyl band around 1730-1735 cm-1 with bands at ~1160 (for acrylic), ~1230 (for PVAc), and 1270 cm-1 (for alkyds). On the other hand, oil/resin binders are characterized by intense and thin νCH2, νCH3 IR absorption bands around 2920-2850 cm-1, combined with an intense 1260 cm-1 band and a characteristic concave cradle shape (between ca. 1750 and 1260 cm-1). The results obtained establish the relevance of the implemented mobile non-invasive infrared spectroscopy analytical approach by successfully identifying acrylic, vinylic, oil media and enamel paints, with or without opacifiers, which is supplemented by Raman analyses for pigment identification.Due to similar charges, atomic radii, and chemical properties of most metal ions, the selective sensing of these metal ions and imaging in vivo is still a challenge. A DNAzyme assemblied and near-infrared (NIR) light excited nanosensor was developed to detect and image Pb2+. In this nanosensor, NaYF4Yb, Er upconversion nanoparticles (UCNPs) introduced as NIR-to-Vis transducer were the donor of luminescence resonance energy transfer (LRET), and DNAzyme-functionalized black hole quencher 1 (BHQ1) acted as energy transfer acceptor. This proposed nanosensor was applied to detecting Pb2+ in solution with high sensitivity and selectivity. Furthermore, we have successfully demonstrated the imaging ability of this nanosensor towords Pb2+ in living cells and early-stage zebrafish with negligible autofluorescence and good photostability. Mezigdomide The UCNP-DNAzyme nanosensor would enrich the method of imaging Pb2+ in vivo, and might serve as a potential tool for understanding the metabolic pathways of Pb2+ and the mechanism of lead poisoning in biological system.Infectious diseases caused by viruses such as SARS-CoV-2 and HPV have greatly endangered human health. The nucleic acid detection is essential for the early diagnosis of diseases. Here, we propose a method called PLCR (PfAgo coupled with modified Ligase Chain Reaction for nucleic acid detection) which utilizes PfAgo to only use DNA guides longer than 14-mer to specifically cleave DNA and LCR to precisely distinguish single-base mismatch. PLCR can detect DNA or RNA without PCR at attomolar sensitivities, distinguish single base mutation between the genome of wild type SARS-CoV-2 and its mutant spike D614G, effectively distinguish the novel coronavirus from other coronaviruses and finally achieve multiplexed detection in 70 min. Additionally, LCR products can be directly used as DNA guides without additional input guides to simplify primer design. With desirable sensitivity, specificity and simplicity, the method can be extended for detecting other pathogenic microorganisms.In this study, porous cage-like hollow magnetic carbon-doped CoO nanocomposite (CoO@C) was successfully synthesized using a metal-organic frameworks (MOFs) as precursor by one-step calcination method in this work. The obtained nanoporous composite showed excellent magnetic response by taking advantage of the magnetism of CoO even without the Fe3O4, making it an advanced sorbent for magnetic solid-phase extraction (MSPE). The Co-MOF and CoO@C were characterized by XRD, TGA, SEM, TEM, vibrating sample magnetometry, and FT-IR spectroscopy. Based on this, a method using CoO@C for MSPE coupled with HPLC was established for the analysis of nine polycyclic aromatic hydrocarbons (PAHs) from various real water samples. The amount of sorbent, extraction times, extraction temperature, desorption times, oscillation rate, and elution volume were optimized. Under the optimal conditions, the method had good relative standard deviations (RSDs) of 1.1%-6.5% and a satisfying linearity range of 0.5-1000 μg L-1. The low LOD and LOQ for nine PAHs were found to be 0.06-1.30 μg L-1 and 0.19-4.30 μg L-1, respectively. The experimental results indicated that the prepared nanocomposite showed excellent adsorption capacity compared to other commercial sorbents and has potential applications for the removal of hazardous pollutants from environmental samples.
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