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Style approach to vast field-of-view image resolution systems employing Gaussian radial foundation functions freeform materials.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a positive-sense single-stranded RNA virus species with a zoonotic origin and responsible for the coronavirus disease 2019(COVID-19). This novel virus has an extremely high infectious rate, which occurs through the contact of contaminated surfaces and also by cough, sneeze, hand-to-mouth-to-eye contact with an affected person. The progression of infection, which goes beyond complications of pneumonia to affecting other physiological functions which cause gastrointestinal, Renal, and neurological complication makes this a life threatening condition. Intense efforts are going across the scientific community in elucidating various aspects of this virus, such as understanding the pathophysiology of the disease, molecular biology, and cellular pathways of viral replication. We hope that nanotechnology and material science can provide a significant contribution to tackle this problem through both diagnostic and therapeutic strategies. But the area is still in the budding phase, which needs urgent and significant attention. This review provides a brief idea regarding the various nanotechnological approaches reported for managing COVID-19 infection. The nanomaterials recently said to have good antiviral activities like Carbon nanotubes (CNTs) and quantum dots (QDs) were also discussed since they are also in the emerging stage of attaining research interest regarding antiviral applications.The COVID -19 outbreak since inception has put the whole world in an unprecedented difficult situation by bringing life around the world to a frightening halt and claiming thousands of lives. Due to COVID-19's spreading across 212 countries globally, an increasing number of infected cases and death tolls rose to 146,841,882, and 3,104,743 (as of April 26, 2021), this remains a real threat to the public health system. This paper presents a novel design for the frequency-domain reconfigurable antenna at Ku and K-bands for satellite-internet of thing (IoT) tracking applications. Four reconfigurable antenna is proposed with the use of four different switch mechanisms. Furthermore, switches are used to change resonance frequency to Ku- and K-bands on the antenna surface with four stages. With the help of the 3D electromagnetic computer simulation technology (CST) studio suite, we model the proposed antenna, perform the simulation with a frequency-domain solver, and validate the results with a time-domain solver with both results obtained in agreement as the proposed reconfigurable antenna operates over a wide frequency range for the satellite-IoT network to track COVID-19 pandemic.A new disease known as COVID-19 caused by the SARS CoV2 virus has engulfed the entire world and led to a global pandemic situation. Till December 9, 2020, the disease has infected 68 million people worldwide and more than 1.56 million people have been killed. The origin of the COVID-19 disease has been traced back to the bats, but the intermediary contact is unknown. The disease spreads by respiratory droplets and contaminated surfaces. In most cases, the virus shows mild symptoms such as fever, fatigue, dyspnea, cough, etc. which may become severe if appropriate precautions are not adhered to. For people with comorbidities (usually elderly) the disease may turn deadly and cause pneumonia, Acute Respiratory Distress Syndrome (ARDS), and multi-organ failure, thereby affecting a person's ability to perform normal breathing which may put them on ventilator support. The virus causes Acute Respiratory Distress Syndrome (ARDS) that can lead to multi-organ failure in the most severe form. A patient suffering from ARspital environment. The challenges related to research in electronic wiring of a mechanical ventilator, the overall structural design, and surrounding base could be appropriately done for different loads by simulating the conditions on tools like ANSYS software with accurate dimensions which could improve their future designs.The global pandemic, COVID-19 needs joint techniques and technology to combat it. The internet of things (IoT) has been at the forefront in solving problems, not only in the health care sector but in other sectors. It delivers accuracy with robustness in the developing service and application. However, it remains clear that the use of IoT is limited to coverage, longevity, security, connectivity issue, immediacy, and multicasting, we proposed in this paper frequency selective surface (FSS) as superstrate for rectangular microstrip antenna. An FSS design combine with the rectangular microstrip antenna for better performance is placed over FSS parallel configuration. The rectangular microstrip antenna was titled 45 degrees to change the band-stop. Analysis of the proposed performance in terms of gain, return loss, and directivity shows that the FSS structure's integration brings better results. With the help of a 3D electromagnetic computer simulation technology CST studio suite, we model the proposed antenna, perform the simulation with a frequency-domain solver, and validate it with a time-domain solver. The proposed impressive result is suitable for satellite networks, which hybrid with IoT can provide a sustainable long-time solution in fighting the COVID-19 pandemic.The deadly corona virus continues to pound the globe mercilessly compelling mathematical models and computational simulations which might prove effective tools to enable global efforts to estimate key transmission parameters involved in the system. We propose a mathematical model using a set of non-linear differential equations to account for the spread of the COVID-19 infection with special compartment class isolation or quarantine and estimate the model parameters by fitting the model with reported data of the ongoing pandemic situation in India. The basic reproduction number is defined and local stability analysis is carried out at each equilibrium point in terms of the reproduction number R 0 . The model is fitted mathematically and makes the data India specific. Additionally, we examined sensitivity analysis of the model. These outcomes recommend how to control the spread of corona, keeping in mind contact and recovery rate. Also we have investigated the elasticity of the basic reproduction number as a measure of control parameters of the dynamical system. Numerical simulations were also done to show that the proposed model is valid for the type and spread of the outbreak which happened in India.Covid 2019 is spreading and emerging rapidly all over the world as a new social disaster. This virus is accountable for the continuous epidemic that causes severe respiratory problems and pneumonia related to contamination of humans, which leads to a dangerous condition of life. Due to the increasing threatening number of cases all over the world, the world health organization (WHO) declared coronavirus as a global health emergency. The pandemic disease affected nearly 80 million people positive cases were reported worldwide till now and cause the death of more than 1.7 million people. The virus has novel characteristics types of pathogens. Many clarifications are done and much more are still unknown and pending. The collaborative research will be useful during this pandemic time in order to meet the improvement of global health improvement. It will also help to know about the knowledge of this COVID-19. Recent advancements in nanotechnology proved that they can help in the production of vaccines in a brief timeframe. In this review, the requirement for quick immunization improvement and the capability and implementation of nanotechnology combat against coronavirus disease were discussed.The on-going SARS-CoV-2 causing COVID-19 discovered in December 2019, is responsible for a global pandemic. The virus belongs to the group of enveloped viruses containing linear, non-segmented, single stranded, positive sense strand RNA as genetic material. Already six different strains Coronaviruses are being reported to infect humans, however the seventh one is genetically similar to the SARS Coronavirus and termed as SARS-CoV-2. Specific crucial macromolecules such as membrane, nuclear, spike and enveloped proteins including HE esterase are present in the virus that interact with ACE2, APN, NEU-5, 9SC2 moiety of humans plays significant role in occurrence and transmission of the devastating disease. This review article summarizes the structure, histopathology, transmission of novel Coronavirus, its symptoms with preventive measures & currently prescribed drugs. Though various drugs and therapy have been administrated or implemented to restrict COVID-19, however it is imperative to develop an antidote against SARS-CoV-2 by the scientific or research community to save life.In vivo characterization of RNA-protein interactions is the key for understanding RNA regulatory mechanisms. Herein, we describe a protocol for detection of proteins interacting with polyadenylated RNAs in the yeast Saccharomyces cerevisiae. Proteins are crosslinked to nucleic acids in vivo by ultraviolet (UV) irradiation of cells, and poly(A)-containing RNAs with bound proteins are isolated from cell lysates using oligo[dT]25 beads. RBPs can be detected by immunoblot analysis or with mass spectrometry to define the mRNA-binding proteome (mRBPome) and its changes under stress. For complete details on the use and execution of this protocol, please refer to Matia-González et al. (2021, 2015).This protocol details the isolation and in vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche. The HSCs do not divide over 7 days and fully retain their long-term functional capacity in transplantation assays. Following hibernation culture, HSCs can be used to study quiescence exit and can be genetically manipulated as single cells prior to division. For complete details on the use and execution of this protocol, please refer to Oedekoven et al. (2021).The regulation of lipid kinases has remained elusive given the difficulties of assessing changes in lipid levels. Here, we describe the isolation of protein and lipid kinases to determine the regulation of lipid kinases in vitro. GS-4224 clinical trial This can be followed by analysis of effects of regulators on lipid kinase-mediated changes in phospholipids without the use of radioactivity, with a specific focus on PI(5)P generation by the enzyme PIKfyve. For complete details on the use and execution of this protocol, please refer to Karabiyik et al. (2021).Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, and TEV protease to make it compatible with minimal laboratory equipment, broadly available in low- and middle-income countries. The enzymes produced with this protocol have been successfully used for molecular detection applications. For complete details on the use and execution of this protocol, please refer to Alcántara et al. (2021a, 2021b).A child with repaired double outlet right ventricle presented with Staphylococcus aureus bacteremia. Despite unsuspecting echocardiography on admission and clinical improvement on antibiotics, repeat routine echocardiography detected an aortic pseudoaneurysm, requiring a Ross-Konno operation. In repaired congenital heart defects with bacteremia, close echocardiographic surveillance is required to detect aortic pseudoaneurysm. (Level of Difficulty Intermediate.).
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