Notes

Declaration of a Dissipative Period Very.
In this work, a novel fluorescence method for the detection of phosphate anions (PO43-) was developed based on porphyrin metalation. Through catalysis by G-quadruplex (G4), Cu2+ could insert into the porphyrins to quench their fluorescence. G4 simultaneously improved the fluorescence of the porphyrins but not that of Cu2+-porphyrin. In the absence of PO43-, the porphyrins were metallized by Cu2+, and no fluorescence was observed. In the presence of PO43-, PO43- could coordinate with Cu2+ to prevent porphyrin metalation. Free porphyrin could bind with G4 to emit strong fluorescence. By comparing four common porphyrins, we found that G4 had the greatest effect on increasing the fluorescence intensity of N-methylmesoporphyrin IX (NMM). BDA-366 research buy Thus, NMM/G4 was chosen for the design of a biosensor. Under optimal experimental conditions, this method showed high sensitivity and satisfactory selectivity for PO43- with a detection limit of 44 nM in a linear range of 0.01-1.0 μM. The recovery experiments showed recovery rates of 93.75-106.00%, suggesting a great potential for measuring PO43- in real samples.Here, we report the existence of a pentameric water cluster in the host framework of [Cu(cyclam)(N3)2]·4H2O, that is stable upto 167 °C, well above the boiling point of water. The pentameric cluster structure embedded in the host framework is evident from the single crystal studies. The high thermal stability is confirmed by TGA and temperature dependent confocal Raman microscopic studies, where loss of water bands is well captured between 167 and 170 °C, besides its existence through SCXRD studies. To the best of our knowledge, this is the first report where temperature dependent confocal Raman microscopic investigation is used to study the stability of water in crystal environment. The study promises that temperature dependent confocal Raman microscopy can be an efficient tool to investigate the existence and stability of small water clusters, precisely in restricted environments.In this study, Raman spectroscopy combined with convolutional neural network (CNN) and chemometrics was used to achieve the identification and quantification of honey samples adulterated with high fructose corn syrup, rice syrup, maltose syrup and blended syrup, respectively. The shallow CNNs utilized to analyze honey mixed with single-variety syrup classified samples into four categories by the adulteration concentration with more than 97% accuracy, and the general CNN model for simultaneously detecting honey adulterated with any type of syrup obtained an accuracy of 94.79%. The established CNNs had the best performance compared with several chemometric classification algorithms. In addition, partial least square regression (PLS) successfully predicted the purity of honey mixed with single syrup, while coefficients of determination and root mean square errors of prediction were greater than 0.98 and less than 3.50, respectively. Therefore, the proposed methods based on Raman spectra have important practical significance for food safety and quality control of honey products.
Kleine-Levin syndrome (KLS) is a rare and debilitating disorder presenting with periodic hypersomnolence, cognitive, psychiatric and behavioral disturbances. In the absence of biomarkers it can be difficult to diagnose. Rare LMOD3 variants in a family and in seven sporadic cases with KLS have been described. Here we report a patient and her family with an unclassified, familial, periodic central disorder of hypersomnolence (CDH) in whom the presence of a LMOD3 gene variant was assessed.

The female patient presented since early adulthood with recurrent episodes of hypersomnolence. Over more than 20 years of follow-up the diagnoses of idiopathic hypersomnia, KLS and hypersomnia associated with a psychiatric condition were made. The family history is positive for periodic hypersomnolence and psychiatric conditions. The patient, her symptomatic mother and her asymptomatic sister carried a Proline for Histidine substitution at codon 552 of the LMOD3-gene. This variant was previously reported in two sporadic KLS patients and its frequency in the general population is below 0.02%.

We report the association of periodic hypersomnia with a polymorphism of the LMOD3-gene in a patient with atypical KLS and a positive family history. Further research is needed to assess the pathological and predictive value of LMOD3 variants in KLS.
We report the association of periodic hypersomnia with a polymorphism of the LMOD3-gene in a patient with atypical KLS and a positive family history. Further research is needed to assess the pathological and predictive value of LMOD3 variants in KLS.Homo erectus s.l. is key for deciphering the origin and subsequent evolution of genus Homo. However, the characterization of this species is hindered by the existence of multiple variants in both mainland and insular Asia, as a result of divergent chronogeographical evolutionary trends, genetic isolation, and interbreeding with other human species. Previous research has shown that cochlear morphology embeds taxonomic and phylogenetic information that may help infer the phylogenetic relationships among hominin species. Here we describe the cochlear morphology of two Indonesian H. erectus individuals (Sangiran 2 and 4), and compare it with a sample of australopiths, Middle to Late Pleistocene humans, and extant humans by means of linear measurements and both principal components and canonical variates analyses performed on shape ratios. Our results indicate that H. erectus displays a mosaic morphology that combines plesiomorphic (australopithlike) features (such as a chimplike round cochlear cross section and low cochlear thickness), with derived characters of later humans (a voluminous and long cochlea, possibly related to hearing abilities)-consistent with the more basal position of H. erectus. Our results also denote substantial variation between the two studied individuals, particularly in the length and radius of the first turn, as well as cross-sectional shape. Given the small size of the available sample, it is not possible to discern whether such differences merely reflect intraspecific variation among roughly coeval H. erectus individuals or whether they might result from greater age differences between them than currently considered. However, our results demonstrate that most characters found in later humans were already present in Indonesian H. erectus, with the exception of Neanderthals, which display an autapomorphic condition relative to other Homo species.Studies of flake tools in the British Lower Paleolithic are rare owing to lower quantities of flake tools than handaxes and the perception that flake tool technology became more important in the succeeding Middle Paleolithic. In Britain, and Europe more broadly, MIS 9 (328-301 ka) has been characterized as a period of technological transition owing to the presence of early prepared core technology and the status of the period as the final interglacial prior to the onset of the Middle Paleolithic. It has been argued that the period demonstrates an increase in both the numbers and importance of flake tools, possibly showing emerging Middle Paleolithic behaviors. This study presents the results of a technological examination of flake tools in Britain during MIS 9, focusing on 25 sites, including 15 assemblages previously recorded as having higher quantities of flake tools. We use these assemblages to assess whether the flake tools of MIS 9 represent a transition toward the technology of the Middle Paleolithic. We consider factors including collection history, site formation, function, reduction, and cultural groups. We argue that in Britain the archaeological record of MIS 9 does not show an increase in the use of flake tools and demonstrates more continuity than change in relation to earlier periods of the Lower Paleolithic. There is a technological background of ad hoc retouch of simple flake tools with occasional evidence of more invasively retouched scrapers. Furthermore, aside from the introduction of Levallois technology, flake tools change little in the Early Middle Paleolithic. These results are contextualized within the broader evidence from Europe and comparisons to the longer sequences at key sites. We conclude that the major changes in technology began between MIS 13 and MIS 11 and these merely became cemented during MIS 9 and the following Middle Paleolithic.
This study assessed whether seizure severity and social anxiety were related to self-disclosure or concealment behaviors independent of felt stigma in Korean adults with epilepsy.

This multicenter, cross-sectional study used the Disclosure Management Scale (DMS), the short forms of the Social Phobia Scale (SPS-6) and Social Interaction Anxiety Scale (SIAS-6), and the Stigma Scale-Revised to evaluate Korean adults with epilepsy. Seizure severity was measured as a composite variable, and multivariate linear regression analyses were performed to assess whether seizure severity and social anxiety were related to concealment behaviors.

The 315 subjects included 132 (41.9%) women and 183 (58.1%) men. Of these subjects, 212 (67.3%) reported rarely or never talking to others about their epilepsy, whereas only 98 (31.1%) stated that they rarely or never kept their epilepsy secret from others. Linear regression analyses showed that concealment behaviors were positively correlated with degree of social anxiety (on separate models using the SIAS-6 and SPS-6) and felt stigma, and negatively associated with level of seizure severity. Both models accounted for about 14% of the variance in DMS scores.

The majority of Korean adults with epilepsy choose concealment and selective disclosure strategies. Social anxiety and seizure severity along with felt stigma play significant roles in deciding whether to disclose or conceal a diagnosis of epilepsy. The explanatory power of these models was weak.
The majority of Korean adults with epilepsy choose concealment and selective disclosure strategies. Social anxiety and seizure severity along with felt stigma play significant roles in deciding whether to disclose or conceal a diagnosis of epilepsy. The explanatory power of these models was weak.Psychogenic nonepileptic seizures (PNES) clinically resemble epileptic seizures (ES) but lack epileptic activity at the time of the seizure and are also not due to any other pathophysiological disorder. The integrative cognitive model (ICM) suggests that PNES is an automatic reaction generated from distorted memory and is perceived as uncontrollable and unwanted. Furthermore, the ICM model implies that a PNES event occurs due to an external or internal cue. Intrigued by this assumption, we wanted to examine why a PNES event occurs at a particular moment by investigating the pre-ictal behavior. This study is a retrospective study. We included all patients diagnosed with PNES or ES admitted to our long-term video-EEG monitoring unit (LTVEM) between 01/01/2018 and 30/08/2020. Using thorough video analysis, we checked the patient's state at the onset of the event and looked back to see what the patient was doing before the event onset. We defined 4 types of pre-ictal behavior A. Inactivity. B. Environment arrangement c.
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