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Organization Involving Serum C1q Cancer Necrosis Factor-Related Protein 9 and the Scientific Characteristics along with Prognosis regarding Ischemic Cerebrovascular event.
Selection of transgenic plants by using genes encoding screenable markers of plant origin with health benefit properties, such as anthocyanin is an important aim in plant genetic engineering. In this study, Malus domestica MYB10 (MdMYB10) gene, was used for Agrobacterium tumefaciens-mediated transformation of two SBS-02 and SBS-04 sugar beet lines. The impact of different light regimes on plant tissue culture from a combination of light, dark/light and dark was investigated. The results of this study showed that the MdMYB10 gene was successfully integrated into the selected purple transgenic lines, suggesting that the expression of MdMYB10 gene in sugar beet shoots can be used as a screenable markers for transformation, possibly replacing antibiotic resistant genes. Furthermore, the results of the antibacterial activity of transgenic plants extracts showed that the total extract obtained from transgenic lines significantly (P 
The online version contains supplementary material available at 10.1007/s13205-022-03120-7.
The online version contains supplementary material available at 10.1007/s13205-022-03120-7.Glycophytic plants are susceptible to salinity and their growth is hampered in more than 40 mM of salt. Salinity not only affects crop yield but also limits available land for farming by decreasing its fertility. Presence of distinct traits in response to environmental conditions might result in evolutionary adaptations. A better understanding of salinity tolerance through a comprehensive study of how Na+ is transported will help in the development of plants with improved salinity tolerance and might lead to increased yield of crops growing in strenuous environment. Ion transporters play pivotal role in salt homeostasis and maintain low cytotoxic effect in the cell. High-affinity potassium transporters are the critical class of integral membrane proteins found in plants. It mainly functions to remove excess Na+ from the transpiration stream to prevent sodium toxicity in the salt-sensitive shoot and leaf tissues. However, there are large number of HKT proteins expressed in plants, and it is possible that these members perform in a wide range of functions. Understanding their mechanism and functions will aid in further manipulation and genetic transformation of different crops. This review focuses on current knowledge of ion selectivity and molecular mechanisms controlling HKT gene expression. The current review highlights the mechanism of different HKT transporters from different plant sources and how this knowledge could prove as a valuable tool to improve crop productivity.2-chloronicotinic acid (2-CA) is a key precursor for the synthesis of a series of pesticides and pharmaceuticals. Nitrilase-catalyzed bioprocess is a promising method for 2-CA production from 2-chloronicotinonitrile (2-CN). In this study, a mutant of nitrilase from Rhodococcus zopfii (RzNIT/W167G) was constitutively overexpressed with Escherichia coli as host, which exhibited a onefold increase in enzymatic activity compared with inducible expression. Biosynthesis of 2-CA using whole cells harboring nitrilase as biocatalysts were investigated and 318.5 mM 2-CA was produced, which was the highest level for 2-CA production catalyzed by nitrilase to date. selleck products 2-CA was recovered from the reaction mixture through a simple acidification step with a recovery yield of 90%. This study developed an efficient bioprocess for 2-CA with great potential for industrial application.
The online version contains supplementary material available at 10.1007/s13205-022-03119-0.
The online version contains supplementary material available at 10.1007/s13205-022-03119-0.This study was done to improve the medicinal properties of Syzygium Aromaticum L by processing S. Aromaticum L. bud essential oil (SABE) to the Nanoemulsion drug delivery system (SABE-NE) and investigating its anti-tumor and apoptotic impacts against the human HT-29 colon cancer cells. Applying the ultra-sonication method and characterization by DLS and FESEM analysis facilitates the nanoemulsification procedure. Human cancer (HT-29) and normal (HFF) cell lines were then evaluated based on the SABE-NE apoptotic and cytotoxic effects. In an in vitro section, flow cytometry method, Cas3 gene profile, AO/PI cell staining, and MTT assays are used to analyze the apoptotic and cytotoxic activities. In further analysis, liver lipid peroxidation and antioxidant genes expression (SOD, CAT, and GPx) investigate alterations in mice organs. As a result, produced 131.2 nm SABE-NE induces apoptosis response and cellular death (Cas3 up-regulation and enhanced SubG1 peaks). Subsequently, the HT-29 cells' viability can reduce significantly, while HFF cells indicate confined cytotoxic impacts. Moreover, in vivo test results on mice livers demonstrate the cytoprotective properties of SABE-NE (reduced lipid peroxidation and increased antioxidant enzymes gene expression and nondetectable cytotoxic impacts). We produced a novel nanoemulsion drug delivery system called SABE-NE, a cell-specific apoptotic inducer. We thus can be utilized as an efficient anti-cancer compound for human colon cancer treatment. link2 However, further supplementary studies are required to verify and approve its cell-specific anti-tumor activity.Colletotrichum falcatum, an ascomycete pathogen causes red rot of sugarcane which is specialized to infect cane stalks. Cellulolytic and pectinolytic enzymes are necessary for degradation of plant cell wall which stands as barrier for successful fungal pathogenesis. In the study, we have confined to the CAZy genes that regulate cellulolytic and pectinolytic enzymes in two distinctive pathotypes of C. falcatum. Comparative transcriptome analysis revealed that a number of CAZy genes producing cellulolytic and pectinolytic enzyme were present in the virulent (Cf671) and least virulent (RoC) pathotypes. link3 Two consecutive transcriptome analyses (in vitro) were performed using Illumina Hi Seq 2500, further analysis was done with various bioinformatic tools. In vitro expression analysis of cutinase, glycoside hydrolyase and pectin-related genes revealed number of genes that attributes virulence. Numerous pectin-related genes involved in degradation of plant cell wall, pectinase and pectin lyase are considered to be key precursor in degradation of pectin in sugarcane. These results suggest that cellulolytic enzymes, cutinase and pectin-related genes are essential for degradation of sugarcane cell wall and considered to be an important pathogenic factor in C. falcatum. This is the first detailed report on sugarcane cell wall-degrading enzymes during its interaction with C. falcatum and also this comparative transcriptome analysis provided more insights into pathogen mechanism on C. falcatum.
The online version contains supplementary material available at 10.1007/s13205-022-03113-6.
The online version contains supplementary material available at 10.1007/s13205-022-03113-6.Litchi is a sub-tropical fruit crop with genotypes that bear fruits with variable seed size. Small seed size is a desirable trait in litchi, as it improves consumers' preference and facilitates fruit processing. Seed specific transcriptome analysis was performed in two litchi genotypes with contrasting seed size to identify the genes associated with seed development. The transcriptomic sequence data from seeds at mid-development stages (16-28 days after anthesis) were de-novo assembled into 1,39,608 Trinity transcripts. Out of these, 6325 transcripts expressed differentially between the two contrasting genotypes. Several putative genes for salicylic acid, jasmonic acid and brassinosteriod pathways were down-regulated in seeds of the small-seeded litchi. The putative regulators of seed maturation and seed storage were down-regulated in the small-seeded genotype. Embryogenesis, cell expansion, seed size and stress related Trinity transcripts exhibited differential expression. Further studies on gene characterization will reveal the early regulators of seed size in litchi.
The online version contains supplementary material available at 10.1007/s13205-021-03098-8.
The online version contains supplementary material available at 10.1007/s13205-021-03098-8.Rind color and pattern are important external fruit quality traits of watermelon influencing the consumer attention and market acceptance. Varying degrees of green and yellow colors in different combinations forming distinct patterns have been observed on watermelon rind. In the current experiment, an attempt has been made to map the QTLs/genes for four important rind traits, namely, stripe color, stripe pattern, interstripe color, and interstripe pattern. The experiment consisted of two mapping populations namely F2 (Pop I) and BC1F2 (Pop II) derived from two parental lines, viz., BIL-53 (characterized by medium green marbled rind pattern and yellowish white blotchy interstripe pattern) and IIHR-140-152 (characterized by dark green solid stripes and greenish wavy interstripe pattern). Linkage mapping identified consistent QTLs across populations on chromosome 9. Comparative genomic analysis of these regions revealed two genes, namely, Cla97C09G175170 and Cla97C09G175150 as potential candidates for stripe and interstripe color. Sequence analysis of Cla97C09G175170 gene in parents along with reference genotypes, viz., 97,103 and Charleston gray suggests a 3 bp deletion on 11th exon to be associated with stripe color polymorphism in watermelon.
The online version contains supplementary material available at 10.1007/s13205-022-03112-7.
The online version contains supplementary material available at 10.1007/s13205-022-03112-7.
Due to the COVID-19 pandemic-related changes and guidelines, nurses in the setting of inpatient geriatric care are exposed to specific burdens and may experience dilemmas with respect to their moral responsibility.

The aim of this study was to explicate the effects and perceptions regarding the development of moral distress of nurses in the setting of geriatric care during the SARS-CoV‑2 pandemic in Germany and to draw conclusions for the design of care in residential care settings.

Based on asubgroup analysis (
 = 510) of the qualitative survey of the COVID-19 Nursing Study, perceptions of nurses working in residential care settings during the pandemic are presented and evaluated with respect to the development of moral distress.

The results show five categories
and "
), which represent differentiated criteria for the development of moral distress among nurses as well as its effects.

It can be concluded that structural and professional solutions must be developed that enable nurses to shape their own working environment and to undertake professional nursing decision-making competence.
It can be concluded that structural and professional solutions must be developed that enable nurses to shape their own working environment and to undertake professional nursing decision-making competence.The demand for breast implant removal (BIR) has increased substantially in recent years. This study leveraged large datasets available through Google Trends to understand how changes in public perception could be influencing surgical demand, both geographically and temporally.
Using Google Trends, we extracted relative search volume for BIR-related search terms in the United States from 2006 to 2019. A network of related search terms was established using pairwise correlative analysis. Terms were assessed for correlation with national BIR case volume based on annual reports provided by the American Society of Plastic Surgeons. A surgical demand index for BIR was created on a state-by-state basis.

A network of internally correlated BIR search terms was found. Search volumes for such terms, including "explant" [ρ = 0.912], "breast implant removal" [ρ = 0.596], "breast implant illness" [ρ = 0.820], "BII" [ρ = 0.600], and "ALCL" [ρ = 0.895] (
< 0.05), were found to be positively correlated with national BIR case volume, whereas "breast augmentation" [ρ = -0.
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