Notes

Characterization involving 3-isopropylmalate dehydrogenase via extremely halophilic archaeon Haloarcula japonica.
However, biodegradation was enhanced during slurry treatment of greenhouse planted soils that received the biosurfactant. The addition of rhamnolipids caused a dramatic shift in the soil bacterial community structure, which was magnified in the presence of sunflower plants. The stimulated groups were identified as fast-growing and catabolically versatile bacteria. This new rhizosphere microbial biomass possibly interacted with the biosurfactant to facilitate intra-aggregate diffusion of PAHs, thus enhancing the kinetics of slow desorption. Our results show that the usually limited biosurfactant efficiency with contaminated field soils can be significantly enhanced by integrating the sunflower ontogenetic cycle into the bioremediation design. In this study, we report an inexpensive, green, and one-pot synthesis method for highly fluorescent carbon quantum dots (CQDs) using mango (Mangifera indica M. indica) leaves to develop an efficient sensing platform for metal ions. The CQDs synthesized from M. indica leaves via pyrolysis treatment at 300 °C for 3 h were characterized by various spectroscopic and electron microscopy techniques for their structural, morphological, and optical properties. Accordingly, the synthesized CQDs showed an absorption peak at 213 nm to confirm the p-p* transition of the carbon core state, while the CQD particles were spherical with a size less than 10 nm. The prepared CQDs showed excellent fluorescent properties with blue emission spectra (around 525 nm) upon excitation at 435 nm. The synthesized CQDs had the prodigious sensing potential to detect Fe2+ ions in water with a limit of detection of 0.62 ppm. Additionally, their sensing capability was tested using a real sample (e.g., Livogen tablet). Moreover, the synthesized CQDs showed substantial stability over a long period (three months). Thus, this study provides an inexpensive and facile method for CQD-based sensing of Fe2+ ions with a photoluminescence quenching mechanism. The prevalence of multidrug-resistant Gram-negative bacteria in aquatic environments has been a long withstanding health concern, namely extended-spectrum beta-lactamase (ESBL) producing Escherichia coli. Given increasing reports on microplastic (MP) pollution in these environments, it has become crucial to better understand the role of MP particles as transport vectors for such multidrug-resistant bacteria. In this study, an incubation experiment was designed where particles of both synthetic and natural material (HDPE, tyre wear, and wood) were sequentially incubated at multiple sites along a salinity gradient from the Lower Weser estuary (Germany) to the offshore island Helgoland (German Bight, North Sea). Following each incubation period, particle biofilms and water samples were assessed for ESBL-producing E. coli, first by the enrichment and detection of E. coli using Fluorocult® LMX Broth followed by cultivation on CHROMAgar™ ESBL media to select for ESBL-producers. Results showed that general E. coli populations were present on the surfaces of wood particles across all sites but none were found to produce ESBLs. Additionally, neither HDPE nor tyre wear particles were found to harbour any E. coli. Conversely, ESBL-producing E. coli were present in surrounding waters from all sites, 64% of which conferred resistances against up to 3 other antibiotic groups, additional to the beta-lactam resistances intrinsic to ESBL-producers. This study provides a first look into the potential of MP to harbour and transport multidrug-resistant E. coli across different environments and the approach serves as an important precursor to further studies on other potentially harmful MP-colonizing species. V.CO2 capture with microalgae has been put forward in response to global concern on greenhouse gas emission. However, the short residence time and slow diffusion of CO2 in water limits the growth of microalgae. In order to improve CO2 transfer from gas phase to liquid phase and utilization by algal cells, polyethylene glycol 200 (PEG 200) was used as CO2 absorbent to promote growth of Nannochloropsis oceanica with the bubbling of 15 vol% CO2. Total inorganic carbon (TIC) absorbed in culture medium remained constant at 5.6 mM when 15 vol% CO2 was bubbled continuously. PEG 200 in the medium provided additional CO2 absorption from 0.6 to 4.8 mM when PEG 200 concentration increased from 0.5 to 4 mM. The specific growth rate of N. oceanica reached the maximum (1.41 d-1) with 1 mM PEG 200 in culture medium, which was 21.5% higher than the specific growth rate without PEG 200. About 79% of the increase in biomass was attributed to the increased TIC with more CO2 dissolution in culture medium because of PEG 200, and about 21% was attributed to PEG 200 itself utilized as an organic carbon source. In conclusion, PEG 200 as a CO2 absorbent can effectively capture flue-gas CO2 for algal growth. Polychlorinated biphenyls (PCBs) exposure is closely associated with the prevalence of metabolic diseases, including fatty liver and dyslipidemia. Emerging literature suggests that disturbance of gut microbiota is related to PCB126-induced metabolic disorders. However, the causal role of dysbiosis in PCB126-induced fatty liver is still unknown. To clarify the role of the gut microbiome in the detoxification of PCB126 in intestine or PCB126-induced toxicity in liver, mice were administrated with drinking water containing antibiotics (ampicillin, vancomycin, neomycin, and metronidazole) or Inulin. We showed that PCB126 resulted in significant hepatic lipid accumulation, inflammation, and fibrosis. PCB126, Antibiotics, and Inulin significantly affected the structure and shifted community membership of gut microbiome. 7 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways at level 2 and 39 KEGG pathways at level 3 were significantly affected. Antibiotics alleviated PCB126-induced fibrosis in the liver but increased inflammation. selleck chemicals llc Inulin treatment ameliorated both inflammation and fibrosis in the liver of PCB126-treated mice. Neither Antibiotics nor Inulin had significant effect on PCB126-induced hepatic steatosis. The more specific intervention of gut microbiota is needed to alleviate PCB126-induced fatty liver. These data demonstrate that homeostasis of gut microbiota is critical for the defense against PCB126 toxicity and dysbiosis plays a fundamental role in the development of inflammation and fibrosis in liver of PCB126-treated mice. V.
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