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Individuals suffering a sport-related concussion typically recover within 1 month; however, persistent post-concussive symptoms are known to occur beyond this period. Clinical guidelines may not be sufficient to determine if dynamic postural control is still impaired at the point of the return to play decision.

Do individuals with a previous sport-related concussion who have returned to play show differences in postural control compared to individuals without a previous concussion, in response to continuous platform perturbations?

Eight previously concussed and eight age- and position-matched participants completed six one-minute trials (three with eyes open/closed) whilst stood on a moving platform that rotated about the pitch axis with a peak-to-peak amplitude of 4° at a frequency of 0.8 Hz. Six trials were also captured during static quiet stance for comparison. Reactive and anticipatory stages of postural control were analysed by determining anteroposterior margins of stability (MoS) as a measure ofform rotations with eyes closed, which could indicate possible lingering deficits to other sensory systems such as the vestibular system, though participants were not likely to lose their balance.
Step count monitors are frequently used in clinical research to measure walking activity. Systematically determining valid days and extracting informative measures of walking beyond total daily step count are among major analytical challenges.

We introduce a novel data-driven anomaly detection algorithm to determine days representing typical walking activity (valid days) and examine the value of measures of walking fragmentation beyond total daily step count.

StepWatch data were collected on 230 adults with severe foot or ankle fractures. Average steps per minute (SC), average steps per active minute (SCA), active to sedentary transition probability (ASTP) and sedentary to active transition probability (SATP) were computed for each participant. The joint distribution of these measures was used to identify and eliminate invalid days through a multi-step process based on the support vector machine. The value of SCA, ASTP and SATP beyond SC were assessed by regressing Short Musculoskeletal Functional Assesprecisely assess activity in clinical studies employing this modality.High cost platinum (Pt) catalysts limit the application of microbial electrolysis cells (MECs) for hydrogen (H2) production. Here, inexpensive and efficient Mo2N nanobelt cathodes were prepared using an ethanol method with minimized catalyst and binder loadings. The chronopotentiometry tests demonstrated that the Mo2N nanobelt cathodes had similar catalytic activities for H2 evolution compared to that of Pt/C (10 wt%). The H2 production rates (0.39 vs. 0.37 m3-H2/m3/d), coulombic efficiencies (90% vs. 77%), and overall hydrogen recovery (74% vs. 70%) of MECs with the Mo2N nanobelt cathodes were also comparable to those with Pt/C cathodes. However, the cost of Mo2N nanobelt catalyst ($ 31/m2) was much less than that of Pt/C catalysts ($ 1930/m2). Furthermore, the biofilm microbiomes at electrodes were studied using the PacBio sequencing of full-length 16S rRNA gene. It indicated Stenotrophomonas nitritireducens as a putative electroactive bacterium dominating the anode biofilm microbiomes. The majority of dominant species in the Mo2N and Pt/C cathode communities belonged to Stenotrophomonas nitritireducens, Stenotrophomonas maltophilia, and Comamonas testosterone. The dominant populations in the cathode biofilms were shaped by the cathode materials. This study demonstrated Mo2N nanobelt catalyst as an alternative to Pt catalyst for H2 production in MECs.In the context of an integrated Lab-on-PCB wearable patch extracting interstitial fluid from the patient via integrated microneedles, the requirements from the integrated biosensing part are quite special compared to static glucose electrochemical biosensors. Hence, in this study, a fully PCB-integrated enzymatic glucose quantification Lab-on-Chip device is presented and evaluated considering these special requirements for such a patch implementation a) range and limit of detection compatible with interstitial fluid glucose levels of diabetic patients and b) effect of sample flow rate on the biosensing platform performance. This work employs a chronoamperometric approach for glucose detection based on covalently immobilized glucose oxidase on PCB-integrated electrodes. The chronoamperometric measurements show that this platform exhibits μM range sensitivity, high specificity, and good reproducibility, and the assay can detect glucose from 10 μM to 9 mM with a lower limit of detection of 10 μM. The demonstrated detection range under continuous flow proved compatible with interstitial fluid glucose levels of diabetic patients. The sample-to-answer time of our Lab-on-PCB device is less than 1 min (sample delivery of few seconds and 20 s for electrochemical measurement), employing sample volumes of 50 μL in this instance. Increased flow rates substantially improve the platform sensitivity (1.1 μA/mM @0 μL/min to 6.2 μA/mM @10 μL/min), with the measured current increasing exponentially to the flow rate, as opposed to the theoretically expected much lower dependence. This work demonstrates the feasibility of Lab-on-PCB patches in terms of biosensing performance, paving the way for the first cost-effective, painless diabetes management microsystem.Construction of novel photoelectrochemical (PEC) materials with unique structures can effectively improve the photoelectric conversion efficiency. Here, a self-supported Cu2O@Cu-MOF/copper mesh (CM) nanobelt arrays with high specific surface area, high orientation, and high photoelectric conversion performance is obtained by in-situ grown strategy. Such PEC aptasensor is constructed based on the Cu2O@Cu-MOF/CM combined with rolling circle amplification and enzymatic biocatalytic precipitation for vascular endothelial growth factor 165 analysis. This strategy achieves excellent cooperative signal amplification, which greatly improves the detection sensitivity. The PEC aptasensor exhibited a wide calibration ranged from 10 to 1 × 108 fM with a detection limit down to 2.3 fM (S/N = 3). The construction of semiconductor@MOFs has developed the potential application of MOFs in photoelectrochemical and found a reliable path for ultrasensitive detection of biomarkers.Surface-enhanced Raman spectroscopy (SERS) is being considered as a powerful technique in the area of food safety due to its rapidity, sensitivity, portability, and non-destructive features. This review aims to provide a comprehensive understanding of SERS applications in fast detection of toxic and harmful substances in food matrix. The enhancement mechanism of SERS, classification of active substrates, detection methods, and their advantages and disadvantages are briefly discussed in the review. The latest research progress of fast SERS detection of food-borne pathogens, mycotoxins, shellfish toxins, illegal food additives, and drug residues are highlighted in sections of the review. According to the current status of SERS detection of food-derived toxic and harmful substances, the review comes up with certain problems to be urgently resolved in SERS and brings up the perspectives on the future directions of SERS based biosensors.Exosomal messenger RNA (mRNA) has emerged as a valuable biomarker for liquid biopsy-based disease diagnosis and prognosis due to its stability in body fluids and its biological regulatory function. Here, we report a rapid one-step isothermal gene amplification reaction based on three-way junction (3WJ) formation and the successful detection of urinary exosomal mRNA from tumor-bearing mice. The 3WJ structure can be formed by the association of 3WJ probes (3WJ-template and 3WJ-primer) in the presence of target RNA. After 3WJ structure formation, the 3WJ primer is repeatedly extended and cleaved by a combination of DNA polymerase and nicking endonuclease, producing multiple signal primers. Subsequently, the signal primers promote a specially designed network reaction pathway to produce G-quadruplex probes under isothermal conditions. Finally, G-quadruplex structure produces highly enhanced fluorescence signal upon binding to thioflavin T. This method provides a detection limit of 1.23 pM (24.6 amol) with high selectivity for the target RNA. More importantly, this method can be useful for the sensing of various kinds of mRNA, including breast cancer cellular mRNA, breast cancer exosomal mRNA, and even urinary exosomal mRNA from breast cancer mice. We anticipate that the developed RNA detection assay can be used for various biomedical applications, such as disease diagnosis, prognosis, and treatment monitoring.The dysregulation of the concentration of individual circulating microRNAs or small sets of them has been recognized as a marker of disease. For example, an increase of the concentration of circulating miR-17 has been linked to lung cancer and metastatic breast cancer, while its decrease has been found in multiple sclerosis and gastric cancer. selleckchem Consequently, techniques for the fast, specific and simple quantitation of microRNAs are becoming crucial enablers of early diagnosis and therapeutic follow-up. DNA based biosensors can serve this purpose, overcoming some of the drawbacks of conventional lab-based techniques. Herein, we report a cost-effective, simple and robust biosensor based on localized surface plasmon resonance and hybridization chain reaction. Immobilized gold nanoparticles are used for the detection of miR-17. Specificity of the detection was achieved by the use of hairpin surface-tethered probes and the hybridization chain reaction was used to amplify the detection signal and thus extend the dynamic range of the quantitation. Less than 1 h is needed for the entire procedure that achieved a limit of detection of about 1 pM or 50 amol/measurement, well within the reported useful range for diagnostic applications. We suggest that this technology could be a promising substitute of traditional lab-based techniques for the detection and quantification of miRNAs after these are extracted from diagnostic specimens and their analysis is thus made possible.In vivo biosensors have a wide range of applications, from the detection of metabolites to the regulation of metabolic networks, providing a versatile tool for cell biology and metabolic engineering. However, compared with the vast array of small molecules present in nature, the existing range of biosensors is far from sufficient. Here we describe the use of human hypoxanthine guanine phosphoribosyltransferase (HGPRT) as a ligand binding domain (LBD) protein, that acts by ligand-dependent stabilization, to build a biosensor for detection of the pentose phosphate pathway metabolite 5-phospho-α-D-ribose 1-diphosphate (PRPP). Using this protein as a template, we computationally redesigned a new pocket de novo according to the pose of the ligand, creating a binding mode exclusive to recognize another pentose phosphate metabolite, D-erythrose 4-phosphate (E4P), and glycerate-3-phosphate (3PG), from the glycolysis pathway. Furthermore, E4P biosensor was developed by fluorescence-activated cell sorting (FACS) and application of it enabled successful screening for the highest phenylalanine-producing strain reported to date.
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