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1 g/L) was necessary to remove 1 mg/L NTMP-P in 30 min. The reusability and effluent polishing potential of the ZnFeZr particles was demonstrated in a pilot test with municipal wastewater throughout 55 adsorption/desorption cycles without any drop in performance. Consistent removal of the non-reactive phosphorus species to ultra-low concentrations ( less then 0.05 mg/L Ptot) and complete orthophosphate elimination ( less then 0.005 mg/L PO4-P) was maintained under optimal conditions.Dredged sediment can occupy a large amount of land area, resulting in waste of land resources, and high disposal costs. In response to the problem, this work calcinates and modified the sediment and compounds it with the modified water purification plant sludge, zeolite powder, and bentonite. This is used as a covering material to inhibit the release of nitrogen (N) and phosphorus (P) in the sediment. The results showed that sediment modified composite material covering effectively reduces the release of nitrogen (N) and phosphorus (P) in the sediment, especially the release of P. When the thickness of the covering layer is 3 cm, the reduction rate of total N, NH4+-N, and total P in the overlying water by the modified composite material of sediment is 61.58, 79.59, and 70.34%, respectively. It can be seen that the covering material has a significant effect on the control of the release of N and P in the sediment. Additionally, the reduction of nutrients in the overlying water can overcome the negative effects of temperature rise in controlling the release of N and P in the sediment.Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction. Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO). Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively. APPO enzyme activity was higher than that of CPPO, while CPPO showed the higher enzyme loading capacity than that of APPO. The removal percentage of phenolic compound, including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by immobilized PPO was also explored. The results indicated that APPO was more efficient in phenolic compounds removal than CPPO. APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively. The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. selleckchem After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively. The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.Enough biomass of anaerobic ammonium oxidation (anammox) bacteria is essential for maintaining a stable partial nitrification/anammox (PN/A) wastewater treatment system. Present enrichment procedures are mainly labor-intensive and inconvenient for up-scaling. A simplified procedure was developed for enrichment of anammox biofilm by using secondary effluent as source water with no supplement of mineral medium and unstrict control of influent dissolved oxygen (DO). Anammox biofilm was successfully enriched in two pilot-scale reactors (XQ-cul and BT-cul) within 250 and 120 days, respectively. The specific anammox activity increased rapidly during the last 2 months in both reactors and achieved 2.54 g N2-N/(m2·d) in XQ-cul and 1.61 g N2-N/(m2·d) in BT-cul. Similar microbial diversity and community structure were obtained in the two reactors despite different secondary effluent being applied from two wastewater treatment plants. Anaerobic ammonium oxidizing bacteria genera abundance reached up to 37.4% and 43.1% in XQ-cul and BT-cul biofilm, respectively. Candidatus Brocadia and Ca. Kuenenia dominated the enriched biofilm. A negligible adverse effect of residual organics and influent DO was observed by using secondary effluent as source water. This anammox biofilm enrichment procedure could facilitate the inoculation and/or bio-augmentation of large-scale mainstream PN/A reactors.Solid-phase denitrification is a promising approach to enhance nitrate removal. In this work, polybutylene succinate (PBS) and peanut shell (PS) (with crosslinked polyvinyl alcohol-sodium alginate (PVA-SA) as carrier) were used to prepare a composite solid carbon source (3P) to denitrify the secondary effluent. The results showed that for carbon release performance, 3P had not only a large release of organics, like PS, but also the excellent sustainability of PBS. Among the short chain fatty acids released by PBS, PS, PVA-SA and 3P, the percentages of acetic acid were 59.42%, 72.54%, 72.29% and 92.11%, respectively. When 3P was used as external carbon source, denitrification performance could be enhanced with effluent dissolved organic carbon lower than 20 mg/L. The prepared 3P could improve denitrification, from both microbial and kinetic aspects. The relative abundance of Gammaproteobacteria increased from 39.32% to 43.58%, and the half saturation constant of the fitting Monod equation was 21.28 mg/L. The prepared 3P is an ideal carbon source for secondary effluent denitrification. Using multiple crosslinking methods to produce carrier is an effective way to show the properties of each material.Attachment and separation of sulfate-reducing bacteria (SRB) biofilm on stainless steel (SS) in simulated cooling water with and without different sterilization treatments was investigated by calculation of surface energy, theoretical work of adhesion and analysis of Scanning Electron Microscope/Energy Dispersive Spectrometer. Two types of biocides, glutaraldehyde and Polyhexamethylene guanidine (PHMG), and electromagnetic treatment were used in this paper. The results show that PHMG had the best bactericidal performance, followed by glutaraldehyde, and electromagnetic treatment was the lowest one. The theoretical work of adhesion was used to quantitatively evaluate the adhesion of biofilm on the surface of the metal. Theoretical work of adhesion between biofilm and SS in simulated cooling water increased with time. The theoretical adhesion work and adhesive capacity of biofilm to SS surface increased after treating with glutaraldehyde while decreasing after treating with PHMG and electromagnetic field. As the theoretical adhesion work decreased, the biofilm was gradually removed from the stainless steel surface.
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