Notes

Extremely Anti-Wetting Colorimetric Starch-Based Video Changed with Poly(dimethylsiloxane) along with Micro-/Nano-Starch with regard to Aquatic-Product Freshness Overseeing.
Pot experiments were carried out to examine the biochar application and its alleviating effect on arsenic (As) toxicity in soybean plants. The data showed that As inhibits the growth indices and it increased with enhanced As-concentration in the substrate. The growth indices declined by more than 40% and the osmolyte concentration, photosynthetic pigments and antioxidant enzymes were decreased significantly among As-stressed plants. However, biochar application effectively mitigated the inhibitory effects of As on the soybean growth and the mitigation effect of treatment is more prevalent to the plants subjected to higher As-treatment. Biochar significantly reduced the As-uptake as revealed by the translocation factor ( less then 1), indicating more As is restrained in the roots. The reduction in the total chlorophyll and carotenoid content was found less in the As-treated soybean plants upon biochar application. Similarly, the osmolytes comprising proline, sugar and protein increased upon application of biochar. The biomarkers viz., membrane stability index (MSI), hydrogen peroxide and malondialdehyde (MDA) content significantly decreased at higher As-levels upon biochar application as was also supported by the heatmap analysis. Moreover, the antioxidative enzymes also showed a significant increase upon addition of biochar. Our data showed that biochar amendment effectively alleviates the As-stress by enhancing the sorption of As in the substrate thus, significantly declining the As concentration in plant leaves, and thus the results of the current study depicting the role of biochar as a promising, cost-effective and eco-friendly amendment to decontaminate the As-polluted soils.The rapidly emerging field of nanotechnology is considered an important achievement in the agriculture sector to increase the pest mortality rate and improve the crop production. The present study evaluates the novel pesticidal and anti-microbial activities of Chrysanthemum coronarium and Azadirachta indica in the nano-suspensions form. The anti-solvent precipitation method was used to formulate nano-suspensions proposed by Response Surface Methodology (RSM). Physicochemical nature of plant extracts and nano-suspensions was characterized through analysis of Zeta-sizer, FT-IR, and HPLC. Characterization results revealed a minimum particle size of 121.1 and 170.1 nm for Chrysanthemum coronarium and Azadirachta indica, respectively. The pesticidal activity of nano-suspension was performed against red flour beetle (RFB) and lesser grain borer (LGB) pests, which showed the maximum mortality rate of 100% with 100% concentration of plant extracts and nano-suspensions of Chrysanthemum coronarium and Azadirachta indica against both insects. In comparison, the combination of these both plant extracts revealed the maximum 100% mortality with a 50% concentration of nano-suspensions (mixing ratio 11) after 72 h. The antibacterial activity showed the maximum zone inhibition of 9.96 ± 0.17 and 14.17 ± 0.50 mm against S.aureus and E. coli with nano-suspension of Chrysanthemum coronarium, and 12.09 ± 0.11 and 14.10 ± 0.49 mm with nano-suspension of Azadirachta indica, respectively. It is concluded that individual nano-suspensions showed better pesticidal as well as antimicrobial activities than combinations. However, the constructed nanosuspension can be applied to control the plant pests and diseases simultaneously.Royal jelly (RJ) is rich in protective elements associated with collective immune defenses in the hive of Apis mellifera. Exposure of bees to glyphosate-based herbicides causes ultrastructural changes in the hypopharyngeal glands and a reduction in the production of RJ. However, the effects of glyphosate-based herbicides on the protein composition of RJ and consequences for the hive are unknown. Thus, we performed proteomic profiling of royal jelly produced in hives of A. mellifera exposed to food containing 1,5 μL of Roundup® (2.16 mg. g-1 of glyphosate). The production of RJ was carried out in six hives, following the method of artificial production of queens. The combs containing 80 grafting cells were introduced into the hives, and the collection of royal jelly was performed after 72 h. Two treatments were determined based on hive feeding and the hive as the experimental unit Control and "Roundup®". Royal jelly from the Roundup® treatment hives was compared to the Control hives. Proteins with differences in expression were identified by mass spectrometry. Only the proteins present in all three biological replicates were considered in the differential abundance analysis, using Student's t-test (p-value less then 0.05, two-tailed). Hives that received food containing Roundup®, analysis showed alterations in protein profile in the RJ produced therein. In total, 24 proteins were identified, and the accumulation of Major royal jelly protein 3 (MRJP3) was downregulated, showing a significant reduction in hives exposed to food containing Roundup® in relation to control hives (t = 0.0017). MRJP3 acts analogously to polyclonal antigen-antibody reactions, performing functions related to immunity in bees of different ages and castes. To the best of our knowledge, this is the first study to demonstrate changes in the proteomic profile of RJ caused by glyphosate-based herbicides, indicating its negative effects on the nutrition and social immunity of bees.Organic anion transporting polypeptide 1B1 (OATP1B1), which is specifically expressed at the basolateral membrane of human hepatocytes, is well recognized as the key determinant in the pharmacokinetics of a wide variety of drugs and considered as an important drug-drug interaction (DDI) site. Triptergium wilfordii Hook. f. (TWHF) is a traditional Chinese medicine that has a long history in treating diseases and more pharmacological effects were demonstrated recently. Components of TWHF mainly belong to the groups of alkaloids, diterpenoids, and triterpenoids. However, whether TWHF constituents are involved in herb-drug interaction (HDI) remains largely unknown. In the present study, we investigated the effect of four major components of TWHF, i.e. Triptolide (TPL), Celastrol (CL), and two alkaloids Wilforine (WFR) and Wilforgine (WFG) on the function of OATP1B1. It was found that co-incubation of these compounds greatly inhibited the uptake function of OATP1B1, with WFG (IC50 = 3.63 ± 0.61 μM) and WFR (IC50 = 3.91 ± 0.30 μM) showing higher inhibitory potency than TPL (IC50 = 184 ± 36 μM) and CL (IC50 = 448 ± 81 μM). Kinetic analysis revealed that co-incubation of WFG or WFR led to the reduction of both Km and Vmax of the DCF uptake. On the other hand, pre-incubation of WFG or WFR increased Km value of OATP1B1; while CL affected both Km and Vmax. In conclusion, co- and pre-incubation of the tested TWHF components inhibited OATP1B1 activity in different manners. Although co-incubation of WFG and WFR did not seem to directly compete with the substrates, pre-incubation of these alkaloids may alter the substrate-transporter interaction.The performance of anaerobic digestion is significantly governed by the concentration of volatile fatty acids (VFAs). Though the titration and near-infrared spectroscopy have been used to measure the VFAs in the digester, there is still lack of the establishment of on-line monitoring of VFAs in practical application. An effective quantification method based on mid-infrared (MIR) spectroscopy was developed, and used to measure the concentrations of VFAs in the anaerobic bioreactor nondestructively in parallel. The wavelet denoising (WD) spectra were used as the spectral preprocessing option. Compared with other pretreatment methods, the established calibration model built by WD spectra showed satisfactory results. Further, the model was verified using high performance liquid chromatography (HPLC), and predictions were made using real reactor effluent samples. Based on this theoretical work, a set of equipment for the in-situ online monitoring of VFAs was designed, which has high feasibility and effectively solves the problems with the current VFAs online monitoring process. These results provide a new solution for on-line monitoring of the anaerobic digestion, and have great potential for practical application.The critical environmental issues of antibiotic resistance and renewable energies supply urge researching materials synthesis and catalyst activity on hydrogen production processes. Aiming to analyse the antibacterial effect of platinum-silver (Ag-Pt) nanoparticles (NPs) and the catalyst effect on NaBH4 hydrolysis that can be used for hydrogen generation technology, in this work, Ag-Pt NPs were prepared using aqueous propolis extract. Various methods were used for the characterization (Uv-vis Spectroscopy, Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), Atomic Force Microscopy (AFM) and X-ray diffraction Spectroscopy (XRD)). The antimicrobial activity of Ag-Pt bimetallic nanoparticles was evaluated in vitro by the microdilution method against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, Staphylococcus epidermidis, and Serratia marcescens. The results confirmed the antimicrobial activity of bimetallic NPs Ag-Pt concentrations of (25, 50, and 100 μg/ml). A concentration of 100 μg/ml showed low bacterial viability varying between 22.58% and 29.67% for the six tested bacteria. For the catalyst activity on NaBH4 hydrolysis, the results showed high turnover factor (TOF) and low activation energy of 1208.57 h-1 and 25.61 kJ/mol, respectively, with high hydrogen yield under low temperature. Synthesized Ag-Pt NPs can have great potential for biological and hydrogen storage applications.Tetracycline hydrochloride (TCH) is a typical antibiotic pollutant with high toxicity and persistence. The degradation of TCH and the generation of the associated electron mediator in a dual chamber microbial fuel cells (MFCs) were studied. The results of liquid chromatography revealed that TCH could be effectively removed (>93%) in MFCs mode. The maximum COD removal was 88.14 ± 1.47% in MFCs while it was 69.57 ± 1.36% in open circuit MFCs. According to cyclic voltammetry, the presence of the relevant redox peaks clearly suggested that the intermediates from TCH degradation could act as endogenous electron mediator. The highest power density of 120.02 ± 2.76 mW/m2 and the lowest internal resistance of 18.68 Ω were achieved in MFC with 2 mg/L of TCH. Microbial community analysis illustrated that Bacteroides, Comamonas, Clostridium_sensu_stricto, Desulfovibrio and Geobacter were enriched and played a dominant role in TCH degradation and power generation. Electrochemical active bacteria had certain tolerance to TCH and the inhibiting threshold value of TCH was below 5 mg/L. This study provided a new thinking that low concentration of TCH could produce electron mediators to improve the performance of MFC system.The inflammasome pathway is a fundamental component of the innate immune system, playing a key role especially in chronic age-related eye diseases (AREDs). The inflammasome is of particular interest because it is a common disease pathway that once instigated, can amplify and perpetuate itself leading to chronic inflammation. Entinostat With aging, it becomes more difficult to shut down inflammation after an insult but the common pathway means that a shared solution may be feasible that could be effective across multiple disease indications. This review focusses on the NLRP3 inflammasome, the most studied and characterized inflammasome in the eye. It describes the two-step signalling required for NLRP3 inflammasome complex activation, and provides evidence for its role in AREDs. In the final section, the article gives an overview of potential NLRP3 inflammasome targeting therapies, before presenting evidence for connexin hemichannel regulators as upstream blockers of inflammasome activation. These have shown therapeutic efficacy in multiple ocular disease models.
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