Notes

Immunogenicity along with efficiency within rodents associated with an adenovirus-based bicistronic rotavirus vaccine revealing NSP4 along with VP7.
Purpose The authors sought to determine the interrater reliability among novice raters of intra-vaginal manual assessment of pubococcygeus muscle tone in women using the Reissing tone scale (RTS). Method Three graduating physiotherapy students (novice raters) and one experienced pelvic floor physiotherapist assessed 31 female participants (aged 20-66 y). Assessors gave RTS scores for pubococcygeus tone at three intra-vaginal locations (600, 900, and 300). Interrater reliability was determined for the novice raters using a two-way random single-measures absolute agreement intra-class correlation coefficient (ICC). Spearman rank correlation (SRC) analysis determined the correlation between the novice and expert scores. Results The ICC values for the novice raters were 0.523, 0.274, and 0.336 at 600, 900, and 300, and the SRC values between the novice and expert raters were 0.580, 0.320, and 0.340. Conclusions The novice raters demonstrated low to moderate interrater reliability for intra-vaginal manual assessment of pubococcygeus tone. This result indicates that manual assessment of pelvic floor muscle tone is not reliable enough to use as a stand-alone test to guide treatment, at least for physiotherapists with limited clinical experience.Purpose This study reports on the prevalence and impact of pain in individuals with different chronic respiratory diseases attending pulmonary rehabilitation (PR). Method A retrospective review of medical records data was conducted for 488 participants who had attended a PR programme over a 2-year period. Data on pain and medication history taken from multidisciplinary medical records, together with participant demographics and PR outcomes, were extracted. We compared pain among participants with different types of chronic respiratory disease. Results The overall prevalence of pain was 77%, with a significantly higher prevalence among individuals with obstructive lung diseases (80%) compared with restrictive lung diseases (69%; p = 0.04). Some participants (17%) who took pain medications did not discuss pain with their clinicians. The presence of pain and different reporting of pain did not have a negative impact on the PR programme completion rate (p = 0.74), improvements in exercise capacity (p = 0.51), or health-related quality of life (all four chronic respiratory disease questionnaire domains, p>0.05). Conclusions The prevalence of pain is high among individuals with chronic respiratory disease attending PR. The presence or absence of pain was not negatively associated with the programme completion rate or PR outcomes; therefore, pain should not deter clinicians from referring patients to PR.The novel and unprecedented Coronavirus disease (COVID-19) pandemic has negatively impacted most nations of the world within a short period. While its disproportionate social and spatial variability has been established, the reality in Nigeria is yet to be studied. In this paper, advanced spatial statistical techniques were engaged to study the burden of COVID-19 and its risk factors within the first quarter (March-May) of its incidence in Nigeria. The spatial autocorrelation (Moran's I) test reveals a significant but marginal cluster of COVID-19 occurrence in Nigeria (I = 0.11, p less then 0.05). A model comparison between ordinary least square (OLS) and spatial error model (SER) was explored having checked for multicollinearity in the dataset. The OLS model explained about 64% (adjusted R2 = 0.64) of variation in COVID-19 cases, however with significantly clustered residuals. The SER model performed better with randomly distributed residuals. The significant predictors were population density, international airport, and literacy ratio. Furthermore, this study addressed the spatial planning implications of the ongoing disease outbreak while it advocates transdisciplinary approach to urban planning practices in Nigeria.
This study investigates how different patterns of nonresident father support for children and mothers in the early years predict middle childhood adjustment, and whether grandparent support has compensating effects.

Nonresident fathers' involvement in children's lives benefits socio-emotional adjustment, but it is unclear whether support directed at children is compromised by interparental tensions, or whether other factors may compensate for weaker patterns of father support.

Latent class analyses identified patterns of nonresident father support for single mothers and their 34-month-old child (None 35%, Low 16%, Moderate 21%, High 28%) and grandparent support (Low 15%, Moderate Maternal 33%, High Maternal 43%, High Maternal and Paternal 9%), using a sample of 648 families from the Growing Up in Scotland cohort. Effects of father support on children's internalizing and externalizing problems from age 46 to 122 months were explored (
=352), together with moderating effects of grandparent support.

Lowfects of weaker father support.
To investigate the pervasiveness and frequency of work schedule unpredictability among workers in low-wage hourly jobs and the effects of work schedule unpredictability on worker and family well-being.

Family science has long considered the ways in which parents' experiences in the workplace can affect families. Although unpredictability in work schedules has increased over time, especially for low-wage workers, the effects of schedule unpredictability on worker and family well-being have been understudied.

Ninety-two workers with children aged 2-7, recruited using a new venue-time sampling technique, were asked to complete once-a-day surveys for 30 consecutive days (
= 2,221 person-days for analysis). Descriptive analyses and regression models with family fixed effects were utilized.

Work schedule unpredictability was common in the context of families' lives over the 30 days, parents experienced an unanticipated work schedule change on 13.3% of days, and 87% of parents experienced at least one unanticipated work schedule change. Within families, unpredictable work schedule changes on a given day were associated with worse outcomes that day for parents, including increased negative mood and decreased perceived sleep quality.

Work schedule unpredictability is ubiquitous in the lives of low-wage hourly workers and is negatively related to working parents' well-being.

These results provide evidence that unscheduled and canceled shifts typical of low-wage service jobs may harm parents' well-being, which could ultimately affect their children's development and well-being.
These results provide evidence that unscheduled and canceled shifts typical of low-wage service jobs may harm parents' well-being, which could ultimately affect their children's development and well-being.Surveys are a crucial tool for understanding public opinion and behaviour, and their accuracy depends on maintaining statistical representativeness of their target populations by minimizing biases from all sources. Increasing data size shrinks confidence intervals but magnifies the effect of survey bias an instance of the Big Data Paradox1. Decitabine inhibitor Here we demonstrate this paradox in estimates of first-dose COVID-19 vaccine uptake in US adults from 9 January to 19 May 2021 from two large surveys Delphi-Facebook2,3 (about 250,000 responses per week) and Census Household Pulse4 (about 75,000 every two weeks). In May 2021, Delphi-Facebook overestimated uptake by 17 percentage points (14-20 percentage points with 5% benchmark imprecision) and Census Household Pulse by 14 (11-17 percentage points with 5% benchmark imprecision), compared to a retroactively updated benchmark the Centers for Disease Control and Prevention published on 26 May 2021. Moreover, their large sample sizes led to miniscule margins of error on the incorrect estimates. By contrast, an Axios-Ipsos online panel5 with about 1,000 responses per week following survey research best practices6 provided reliable estimates and uncertainty quantification. We decompose observed error using a recent analytic framework1 to explain the inaccuracy in the three surveys. We then analyse the implications for vaccine hesitancy and willingness. We show how a survey of 250,000 respondents can produce an estimate of the population mean that is no more accurate than an estimate from a simple random sample of size 10. Our central message is that data quality matters more than data quantity, and that compensating the former with the latter is a mathematically provable losing proposition.Collective cell migration underlies morphogenesis, wound healing and cancer invasion1,2. Most directed migration in vivo has been attributed to chemotaxis, whereby cells follow a chemical gradient3-5. Cells can also follow a stiffness gradient in vitro, a process called durotaxis3,4,6-8, but evidence for durotaxis in vivo is lacking6. Here we show that in Xenopus laevis the neural crest-an embryonic cell population-self-generates a stiffness gradient in the adjacent placodal tissue, and follows this gradient by durotaxis. The gradient moves with the neural crest, which is continually pursuing a retreating region of high substrate stiffness. Mechanistically, the neural crest induces the gradient due to N-cadherin interactions with the placodes and senses the gradient through cell-matrix adhesions, resulting in polarized Rac activity and actomyosin contractility, which coordinates durotaxis. Durotaxis synergizes with chemotaxis, cooperatively polarizing actomyosin machinery of the cell group to prompt efficient directional collective cell migration in vivo. These results show that durotaxis and dynamic stiffness gradients exist in vivo, and gradients of chemical and mechanical signals cooperate to achieve efficient directional cell migration.Cigarette smoking constitutes a leading global cause of morbidity and preventable death1, and most active smokers report a desire or recent attempt to quit2. Smoking-cessation-induced weight gain (SCWG; 4.5 kg reported to be gained on average per 6-12 months, >10 kg year-1 in 13% of those who stopped smoking3) constitutes a major obstacle to smoking abstinence4, even under stable5,6 or restricted7 caloric intake. Here we use a mouse model to demonstrate that smoking and cessation induce a dysbiotic state that is driven by an intestinal influx of cigarette-smoke-related metabolites. Microbiome depletion induced by treatment with antibiotics prevents SCWG. Conversely, fecal microbiome transplantation from mice previously exposed to cigarette smoke into germ-free mice naive to smoke exposure induces excessive weight gain across diets and mouse strains. Metabolically, microbiome-induced SCWG involves a concerted host and microbiome shunting of dietary choline to dimethylglycine driving increased gut energy harvest, coupled with the depletion of a cross-regulated weight-lowering metabolite, N-acetylglycine, and possibly by the effects of other differentially abundant cigarette-smoke-related metabolites. Dimethylglycine and N-acetylglycine may also modulate weight and associated adipose-tissue immunity under non-smoking conditions. Preliminary observations in a small cross-sectional human cohort support these findings, which calls for larger human trials to establish the relevance of this mechanism in active smokers. Collectively, we uncover a microbiome-dependent orchestration of SCWG that may be exploitable to improve smoking-cessation success and to correct metabolic perturbations even in non-smoking settings.
Website: https://www.selleckchem.com/products/Decitabine.html