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Case Document: Esophageal Bronchus in a Neonate, Using Graphic, Histological, as well as Molecular Investigation.
Magnetic titanium dioxide nanoparticles modified with green deep eutectic solvent (DES) composed of choline chloride (ChCl) and xylitol (Xyl) (Fe3O4@TiO2@[ChCl][Xyl]) were synthesized and applied to the solid-phase extraction(MSPE) of chymotrypsin (Chy). The physicochemical properties and morphology of Fe3O4@TiO2@[ChCl][Xyl] was characterized by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), Zeta potential, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) and transmission electron microscope (TEM). The experiment parameters such as initial concentration of Chy, extraction time, pH value, ionic strength, extraction temperature and sample matrix were effectively optimized. Under the optimal experimental conditions, the extraction capacity of Fe3O4@TiO2@[ChCl][Xyl] obtained a significantly improvement after the modification of Fe3O4@TiO2 nanoparticles by [ChCl][Xyl], and reached up to 347.8 mg g-1. In the elution experiment, 10% sodium dodecyl sulfate-acetic acid (SDS-HAc) was used as eluent, achieving an elution rate of 85.9% for the Chy on Fe3O4@TiO2@[ChCl][Xyl]. And the Fe3O4@TiO2@[ChCl][Xyl] still maintained a good extraction capacity for Chy after six times of reuse. The application result in the extraction of Chy from porcine pancreas crude extract showed a good practical application ability for Chy extraction. All the results indicated that the synthesized Fe3O4@TiO2@[ChCl][Xyl] has good application potential in the extraction of biomolecular molecules such as protein.Interferons are important biomolecules in human immune system. Cytokine interferon alpha (IFN-α), a type I interferon, is one of the major components of the innate immune response involved in autoimmune diseases. Thus, the analysis of interferons is of great importance for both biological and pharmaceutical purposes. In this work, an IFN-α specific plastic antibody is prepared via boronate affinity oriented surface imprinting. By combing with the magnetic nanoparticles, the imprinted material exhibits several advantages, including strong affinity (Kd 75.2 nM), high specificity (cross reactivity less then 25%), excellent efficiency (imprinting efficiency 44.1%), tolerance to interferences, and easy manipulation. By employing the prepared imprinted material as sorbent for selective enrichment of IFN-α, a good linearity is achieved in the range of 50 ng/mL-10 μg/mL, and the detection and quantifcation limits are 10 ng/mL and 50 ng/mL respectively. The recoveries of this approach are found within 75.8%-82.2% with relative standard deviations of 6.4-9.7%. Furthermore, the IFN-α in spiked human serum is analyzed with acceptable reliability (recovery 77.3%, RSD 7.9%). Because of these highly desirable properties, the IFN-α specific plastic antibody can find more applications in medical and pharmaceutical industry.The regular design of atomic composition of materials endows a diverse range of enzyme mimics types and increases a broader application prospect. In this study, we designed a nickel-cobalt mixed metal sulfide and demonstrated that the as-prepared NiCo2S4 microflowers possessed intrinsic peroxidase-like activity. Nickel-cobalt sulfide (NiCo2S4) possessed high electron transfer capacity, which lead to good peroxidase-like activity. Compared with the reported enzyme-like materials, NiCo2S4 exhibited a smaller Km and a stronger affinity with substrate. A colorimetric assay was developed for the direct detection of hydrogen peroxide and indirect detection of glucose over a wide linear range (H2O2 was 20-200 μM, and glucose was 20-1000 μM) with a low detection limit (H2O2 was 5.19 μM, and glucose was 8.24 μM). Furthermore, a NiCo2S4 based platform was established to study the antioxidant behavior of three antioxidants. The antioxidant capacities of the antioxidants were found to rank in the order tannic acid (TA) > ascorbic acid (AA)> gallic acid (GA). Moreover, the three antioxidants showed different inhibition mechanisms. This study indicated a new and important application field for NiCo2S4 and provides a basis for the rational design of enzyme-like mimics in future research.C- reactive protein (CRP) is a sensitive indicator for infectious or inflammatory diseases in human which can reflect the body's inflammation latency and early pathophysiological changes. The most common detection method of serum CRP is ELISA that has been proved to be expensive and time-consuming, restricting its use in point-of-care application. In this paper, we demonstrated a lateral flow system for CRP quantification by using mesoporous silica (mSiO2) coated up-converting nanoparticles (UCNPs) (denoted as UCNPs@mSiO2) as fluorescent labels. The up-converting core can emit strong green fluorescence signals under NIR excitation light (980 nm) with excellent photostability, high signal-to-noise ratio and low background fluorescence. By wrapping ultrathin mSiO2 outside, the core-shell structured UCNPs@mSiO2 exhibits good dispersity and stability meanwhile maintains strong fluorescence emission. Besides, the mSiO2 shell provides further functionalities for antibody linkage. By using a portable fluorescence sensor, we reached a CRP detection limit of 0.05 ng/mL and a linear range from 0.1 ng/mL-50 ng/mL, and the detection time was no more than 8 min. The lateral flow test strips exhibit great stability in CRP quantification (CV% less then 5) and have a life time of more than 1 week at ambient temperature. Furthermore, the proposed system can work with a cloud-enabled smartphone through Bluetooth for Internet of Medical Things application. This CRP detection method proves to be rapid and easy-operated, which has great potential in early inflammatory disease perception in the point-of-care tests and future's 5G-enabled remote healthcare management.Column technology is an important part in capillary electrochromatographic science. Developing novel stationary phase with high separation efficiency and high loading capacity is an essential work. In this work, a novel spherical vinyl-functionalized covalent-organic framework (COF-V) was synthesized at room temperature and firstly employed as stationary phase for CEC-MS analysis. The COF-V based CEC column was characterized by scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. The results proved the successful modification of COF-V. The COF-V based column possesses the advantages like strong electroosmotic flow, high separation efficiency and high loading capacity. The CEC column showed powerful separation selectivity to several kinds of compounds, and the highest column efficiency (theoretical plates, N) was over 1.4 × 105 plates·m-1 for methylbenzene. Besides, the COF-V modified column exhibited excellent repeatability and stability. The relative standard deviations (RSDs) of retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-column (n = 3) were all less than 2.1%. Hence, the COF-V modified column was successfully applied in CEC-MS for determination of antiepileptic drug, triazine herbicides and active ingredients in traditional Chinese medicine.There is a growing urgent requirement for miniaturized laser-induced fluorescence (LIF) detection systems in many research fields. In this work, miniaturized LIF detectors with three different optical configurations of orthogonal, confocal, and oblique were developed, using a laser diode as the excitation source and a photodiode as the photodetector. The computer simulation and experimental methods were used to investigate the distributions of laser scattered light and fluorescent light near the detection window. Other conditions including the solution preparation, sample flow rate, alignment method and filter model were also optimized. Under the optimized conditions, the detection limits of sodium fluorescein for orthogonal and confocal LIF detectors were 40 pM and 50 pM, respectively, while the limit of detection (LOD) for oblique LIF detector were 1 nM (45°) and 7 nM (67.5°). We further built a fully integrated handheld orthogonal LIF detector with a total size of 50 × 20 × 46 mm3, a cost of $380, and a detection limit of 10 pM for sodium fluorescein. It is expected that such a LIF detector could be applied in field analysis as a portable instrument or in other analysis systems as a detection module.Scutellaria baicalensis is one of the widely used Chinese traditional medicines, and wogonin is one of major active components in it. However, the mechanism of action of wogonin has largely remained unclear. In this work, we designed a fluorescent probe, namely ATTO565-WGN, by conjugating wogonin with the fluorophore ATTO565 based on Mannich reaction via a flexible chain linker. In vitro assays verified that the ATTO565-WGN conjugate has a similar anti-proliferative activity to wogonin against human A549 and HeLa cancer cell lines. Combining co-localization and competition studies, confocal fluorescence imaging clearly demonstrated that the fluorescent wogonin probe predominantly located in mitochondrial area of living cells, indicating that wogonin acts at mitochondrion to exert its pharmacological functions. Significantly, the conjugated ATTO565 fluorophore conferred the wogonin probe STED (Stimulated Emission Depletion) feature, enabling STED fluorescence living cell imaging with a 55 nm of ultrahigh spatial resolution. This will greatly beneficial for the in situ investigation of interactions between wogonin and biological targets at the finely organized and dynamic mitochondria of living cells. Moreover, this work also provides novel insights into rational design of mitochondrion targeting fluorescence probes for ultrahigh resolution living cell imaging.The complex natural organic matter of the Suwannee River fulvic acid (SRFA) standard was analyzed by online reversed-phase chromatography with Orbitrap MS/MS using collision-induced dissociation (CID). The number of isobars per nominal mass could be reduced to a single dominantly abundant species in a chromatographic run, sharing some ions with signals having the identical molecular formula in adjacent chromatographic segments and later serving as a precursor ion for fragmentation. A very large proportion of the same fragment ions existed in adjacent chromatographic fractions. The difference in the fragment ions in adjacent chromatographic fractions could be attributed to a gradual change in the formula composition of precursor ions in a chromatographic run. It could be concluded that dissolved organic matter (DOM) molecules with the same elemental composition in different chromatographic fractions may have very similar molecular structures. In addition, we propose a possible DOM model that might greatly deepen our understanding of the behavior of DOM in aquatic matrices.Extensive use of nanomaterials in consumer products inevitably leads to their release to the environment. For monitoring of the fate of nanoparticles in various matrices, low-cost, simple and rapid size-based methods are required. Graphite furnace atomic absorption spectrometry (GFAAS) can be used for this purpose, because due to the difference between a thermal energy required to atomize ionic silver (Ag+) and silver nanoparticles (AgNPs) of different size, the maxima of signals measured by are slightly shifted in time. However, signals are seriously overlapped. Analytical signals of various concentrations of Ag+ and differently sized AgNPs in their mixtures were registered under optimized temperature conditions using GFAAS. The research aim was to develop a procedure for quantitative and qualitative analyses of Ag species in the binary mixtures without the need for the deconvolution of their signals into single components. PF-562271 research buy For evaluation of the qualitative composition of the silver forms present in the studied samples, soft independent modeling of class analogy (SIMCA) was applied.
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