Notes

The reformulation involving time-dependent Kohn-Sham principle due to the subsequent period offshoot with the denseness.
Polycystic ovary syndrome (PCOS) is a complicated endocrine and metabolic abnormality diseases common in women of child-bearing age. This study aims to screen out critical miRNAs and mRNAs associated with PCOS, which may be conducive to offer novel insights and treatment for the diseases.

Three mRNA datasets and one miRNA dataset derived from granulosa cells of patients with PCOS and normal controls were downloaded to obtain the differentially expressed mRNAs (DEmRNAs) and miRNAs (DEmiRNAs). Then, DEmiRNA-target DEmRNAs analysis and functional annotation of DEmiRNA-target DEmRNAs were performed. Quantitative real time polymerase chain reaction (qRT-PCR) validation of the expression of the selected DEmRNAs and DEmiRNAs were performed.

A total of 1643 DEmRNAs, 88 DEmiRNAs, 2406 DEmiRNA (down)-DEmRNA (up), and 2179 DEmiRNA (up)-DEmRNA (down) pairs were obtained. The functional annotation of DEmiRNA-target DEmRNAs revealed that C-type lectin receptor signaling pathway, Steroid biosynthesis and Galactose metabolism were significantly enriched KEGG pathways.

These findings may provide make contribution to understanding PCOS pathogenesis, diagnosis, or treatment.
These findings may provide make contribution to understanding PCOS pathogenesis, diagnosis, or treatment.
To minimize the adverse events of uterine compression suture in controlling postpartum hemorrhage (PPH) and to search for a prophylactic approach to potential PPH.

A retrospective analysis was performed in 39 women with removable retropubic uterine compression suture (RRUCS) to stop PPH due to uterine atony during cesarean section (CS). The procedure was to suspend and compress the uterus to the retropubic abdominal wall using an absorbable suture.

The technique was sufficient to stanch bleeding immediately in 36 patients (92.31%, 36/39). No morbidity or abnormalities occurred in women who underwent RRUCS. Subsequent pregnancies occurred in 10 cases, but the others lacked the desire for future pregnancy.

RRUCS is a simple, safe, and effective technique in controlling atonic PPH; it is also used as a prophylactic application in patients with potential PPH after CS.
RRUCS is a simple, safe, and effective technique in controlling atonic PPH; it is also used as a prophylactic application in patients with potential PPH after CS.
The aim of this study was to assess the effect of vibration stimulation application in ventrogluteal region on intramuscular injection pain and patient satisfaction.

Intramuscular injection pain caused distress for the patients and affected their compliance with the treatment.

This was a prospective, single-blind, cross-over study. The study complied with the guidelines of Consolidated Standards of Reporting Trials (CONSORT).

Eighty-four patients who applied to the adult emergency department received an intramuscular injection of amoxicillin cefuroxime sodium to the ventrogluteal site with and without vibration in a random order following a standard procedure by the same investigator. Pain and satisfaction were assessed immediately after the injection with Visual Analogue Scale (VAS), by a researcher blinded to the study. The data were evaluated using mean, standard deviation, percentage, Student's t-test, paired sample t-test, linear regression analysis and generalised linear mixed model.

A total of 174 injections were analysed. The result of the study revealed that vibration decreased the severity of pain and increased the patient satisfaction.

Vibration is a non-pharmacologic approach, which can be effective in decreasing the injection pain and increasing patient satisfaction.

Intramuscular injection is the most frequently used nursing practice in the clinic. The use of vibration in decreasing pain due to intramuscular applications can help increase the self-confidence of nurses and the quality of the care they provide.
Intramuscular injection is the most frequently used nursing practice in the clinic. The use of vibration in decreasing pain due to intramuscular applications can help increase the self-confidence of nurses and the quality of the care they provide.The objective of this experiment was to determine the effects of feeding varying levels of crude glycerine (CG) on dry matter intake (DMI), animal performance, carcass traits and rumen volatile fatty acids (VFA) concentration in meat goats. Twenty-four intact male Boer goats (23.9 ± 1.0 kg initial BW and 4-5 months of age) were randomly assigned to one of four experimental diets (n = 6) containing 30% bermudagrass hay plus 70% concentrate mix with 0, 5, 10 or 15% CG in the diet on an as-fed basis, substituted for the corn portion of the concentrate. Feed offered and refusals were monitored daily for 84 days. Goats were weighed at 28-days interval. Blood and rumen samples were collected on day 84. At the end of the 84-days feeding period, goats were harvested, and carcass characteristics measured. Feed intake, average daily gain (ADG), gain-to-feed ratio (GF), carcass traits and VFA concentrations were analysed as a completely randomized design. The CG did not influence animal body weight (BW) changes, ADG and GF ratio but tended to (p = 0.06) decrease DMI. Molar per cent propionic acid increased linearly (p less then 0.05) with increasing CG. The acetate propionate (AP) ratio decreased (p less then 0.05) with increasing amounts of CG in the diet. The CG addition tended to increase (p = 0.09) the longissimus muscle (LM area) in meat goats. In conclusion, CG can replace corn in diet for growing meat goats when fed up to 15% of diet, improving ruminal propionate concentration, but decreasing AP ratio without affecting animal performance and carcass traits.Rapid quantification of viruses is vital for basic research on viral diseases as well as biomedical application of virus-based products. Here, we report the development of a high-throughput single-particle method to enumerate intact viral particles by ultrasensitive flow virometry, which detects single viruses as small as 27 nm in diameter. The nucleic acid dye SYTO 82 was used to stain the viral (or vector) genome, and a laboratory-built nano-flow cytometer (nFCM) was employed to simultaneously detect the side-scatter and fluorescence signals of individual viral particles. Using the bacteriophage T7 as a model system, intact virions were completely discriminated from empty capsids and naked viral genomes. Successful measurement of the physical virus titer and purity was demonstrated for recombinant adenoviruses, which could be used for gene delivery, therapeutic products derived from phage cocktails, and infected cell supernatants for veterinary vaccine production.
To develop a free-breathing cardiac self-gated technique that provides cine images and



B


1


+


slice profile-corrected T
maps from a single acquisition.

Without breath-holding or electrocardiogram gating, data were acquired continuously on a 3T scanner using a golden-angle gradient-echo spiral pulse sequence, with an inversion RF pulse applied every 4 seconds. MMAF Flip angles of 3° and 15° were used for readouts after the first four and second four inversions. Self-gating cardiac triggers were extracted from heart image navigators, and respiratory motion was corrected by rigid registration on each heartbeat. Cine images were reconstructed from the steady-state portion of 15° readouts using a low-rank plus sparse reconstruction. The T
maps were fit using a projection onto convex sets approach from images reconstructed using slice profile-corrected dictionary learning. This strategy was evaluated in a phantom and 14 human subjects.

The self-gated signal demoingle acquisition without the need for electrocardiogram gating or breath-holding. This dual-excitation flip-angle approach provides a novel approach for measuring T1 while accounting for B 1 + and slice profile effect on the apparent T 1 ∗ .While the effects of climate (long-term, prevailing weather) on species abundance, range and genetic diversity have been widely studied, short-term, localized variations in atmospheric conditions (i.e., weather) can also rapidly alter species' geographical ranges and population sizes, but little is known about how they affect genetic diversity. We investigated the relationship between weather and range-wide genetic diversity in a marsupial, Bettongia gaimardi, using dynamic species distribution models (SDMs). Genetic diversity was lower in parts of the range where the weather-based SDM predicted high variability in probability of B. gaimardi occurrence during 1950-2009. This is probably an effect of lower population sizes and extinction-recolonization cycles in places with highly variable weather. Spatial variation in genetic diversity was also better predicted by mean probabilities of B. gaimardi occurrence from weather- than climate-based SDMs. Our results illustrate the importance of weather in driving population dynamics and species distributions on decadal timescales and thereby in affecting genetic diversity. Modelling the links between changing weather patterns, species distributions and genetic diversity will allow researchers to better forecast biological impacts of climate change.Direct oxidative C(sp)-H/C(sp3 )-H cross-coupling offers an ideal and environmentally benign protocol for C(sp)-C(sp3 ) bond formations. As such, reactivity and site-selectivity with respect to C(sp3 )-H bond cleavage have remained a persistent challenge. Herein is reported a simple method for iron-catalyzed/silver-mediated tertiary alkylation of terminal alkynes with readily available and versatile 1,3-dicarbonyl compounds. The reaction is suitable for an array of substrates and proceeds in a highly selective manner even employing alkanes containing other tertiary, benzylic, and C(sp3 )-H bonds alpha to heteroatoms. Elaboration of the products enables the synthesis of a series of versatile building blocks. Control experiments implicate the in situ generation of a tertiary carbon-centered radical species.
The objective of this study was to isolate a bacteriocin-producing strain and to characterize the expressed bacteriocin for the control of Listeria monocytogenes with aim of biopreservation application.

Soil samples from a Korean organic farm were subjected to microbiological analysis for isolation of potential bacteriocinogenic LAB, based on a three-level approach, using L. monocytogenes ATCC 15313 as an indicator test micro-organism. From a total of 17 isolates with inhibitory potential, seven were confirmed to be bacteriocin producers. The selected isolates were differentiated based on their morphology, catalase reaction, sugar fermentation profile obtained by API50CHL and by RAPD-PCR generating two unique profiles. One of the isolates, ST110LD, a specific strong producer of anti-Listeria bacteriocins (12800AUml
) was identified as Leuconostoc citreum. The proteinaceous nature of the inhibitory compound produced by Leuc. citreum ST110LD was confirmed through treatment with pepsin and α-chymotrypsin. and Enterococcus sp.
To the best of our knowledge, this is the first report of bacteriocin produced by Leuc. citreum strain with highly specific antimicrobial activity against Listeria sp. and Enterococcus sp.
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