Notes

[Unexpected recurrence of malaria].
A meta-regression was performed to evaluate heterogeneity (MES variance) among studies. The statistical analysis was performed using a robust variance estimation strategy with a SAS macro. Probiotic-supplemented birds had a significantly higher BWG (MES = 1.04, P = 0.009) and a significantly lower FCR (MES = -1.39, P = 0.020), NE mortality (MES = -1.15, P = 0.012), and LS (MES = -1.29, P = 0.045). Response variables of BWG (Q = 2.81, P = 0.560) and NE mortality (Q = 5.60, P = 0.354) did not present heterogeneity. Heterogeneity was found for FCR (Q = 10.34, P = 0.035) and LS (Q = 16.13, P = 0.001). Overall, dietary supplementation of B. subtilis DSM 32315 significantly improved BWG and reduced FCR, mortality, and LS in a repeatable large-scale manner.The present study was designed to analyze the histologic and cytologic changes of lymphocyte homing in noninfected and duck Tembusu virus (DTMUV)-infected duck spleens. At first, we investigated the noninfected structure that facilitates lymphocyte homing. Under light and electron microscopy, results showed that sheath capillaries were located in the white pulp of the spleen, and the endothelial cells of sheath capillaries were cuboidal in shape, which is a typical characteristic of high endothelial venules. To monitor the lymphocyte homing, 5,6-carboxy fluoresceindiacetate succinimidyl ester (CFSE)-labeled lymphocytes that were intravenously injected into noninfected ducks appeared in the periellipsoidal sheaths (PELS), which proved that lymphocytes can return to the spleen through sheath capillaries. Furthermore, proteoglycans (PGs) associated with homing factors were positively observed in sheath capillaries and PELS by colloidal iron staining. This suggests that PGs are associated with lymphocyte homing. The results of the DTMUV infection experiment showed that PELS appeared vacuolized at 3 dpi. The spleen tissue gradually recovered at 5 and 7 dpi. In addition, the lymphocytes increased around sheath capillaries, and the expression of PGs in sheath capillaries increased after virus infection. Meanwhile, the gaps between endothelial cells were enlarged, and the lymphocytes were mainly in the lumen and basement membrane. In conclusion, lymphocytes could recruit into the spleen through sheath capillaries, and PGs participated and promoted the lymphocyte homing, suggesting that the unique high endothelial capillaries favor lymphocyte homing, which promotes tissue repair and antigen clearance in the duck.This study investigated the effects of a proprietary commercial feed additive (FA) comprised of a blend of fatty acids, organic acids, and phytochemicals; a hydroxychloride copper (MA); as well as a water acidification product (WA), alone and in combination, on growth performance in nonvaccinated broiler chickens raised in an antibiotic-free production system. The test treatments were FA; WA; FA and WA combined (FA + WA); and FA, WA, and MA combined (FA + WA + MA). The efficacy of these treatments was compared with a negative control (CON) and a medicated feeding program (bacitracin, antibiotic growth promoter [AGP]). Ross 708 cockerels (n = 2376) were subject to a 3-phase commercial feeding program, namely, starter (0-20 days), grower (21-28 days), and finisher (28-35 days), with no coccidiostats or additional medications added to the feed. On day 14, birds were subjected to an in-feed Clostridium perfringens challenge and a subset of animals were euthanized and the ileal digesta was collected for C. perfringens enumeration. Prior to pathogen challenge (day 14), birds fed the FA + WA and F + WA + MA treatments had significantly higher body weights (+2.6%-3.5%) than those fed CON and similar body weights to birds fed the AGP. SW033291 manufacturer These early growth advantages were not sustained postchallenge. Clostridia counts in ileal digesta were dramatically reduced in birds fed the AGP compared with all treatments. The FA (-2.5 log), FA + WA (-2.0 log), and FA + WA + MA (-2.3 log) treatments had significantly lower clostridia counts than the CON treatment. Together, these findings support the use of combined in-feed and in-water strategies for reducing clostridia, while maintaining growth, in antibiotic-free production systems.Clostridium perfringens, a commensal of the intestinal tract of many animal species, has been associated with necrotic enteritis (NE), an economically significant poultry disease. Clostridium perfringens is known to survive in the environment for extended periods of time through the formation of spores. These spores have the potential to be transmitted to subsequent flocks. Persistence of a single C. perfringens strain in a broiler chicken farm environment has, however, been poorly documented. The aim of this study was to compare multiple isolates of C. perfringens collected over time in a single farm with recurrent episodes of NE. Isolates were recovered from the intestines of chickens affected with NE (2014 and 2016 outbreaks) and from healthy chickens (2017), as well as from environmental samples (2016). PCR characterization of those isolates showed that all sampling groups contained netB-positive isolates except for the environmental samples. Moreover, results showed that all environmental isolates were positive for the cna adhesin whereas other groups had lower numbers of cna-positive isolates. Biofilm formation assays showed that most of the isolates were able to form biofilm. Pulsed-field gel electrophoresis analysis showed that one clone was present in every sampling group, with the exception of the 2014 outbreak. However, one clone found in the latter group was highly similar, having 94% similarity with the persistent C. perfringens clone. This study describes for the first time the persistence of a C. perfringens strain on a broiler chicken house over a 3-yr period.Although poultry microbiome discoveries are increasing due to the potential impact on poultry performance, studies examining the poultry respiratory microbiome are challenging because of the low microbial biomass and uniqueness of the avian respiratory tract, making it difficult to sample enough material for microbial analysis. Invasive sampling techniques requiring euthanasia are currently used to increase microbial mass for the analysis, thus making it impossible to sample individual birds longitudinally. In this study, we compared invasive (nasal wash, upper tracheal wash, lower tracheal wash, and lower respiratory lavage) and noninvasive (tracheal and choanal swabs) respiratory sampling techniques in two independent experiments by using 4-wk-old chickens. We first established the experimental baseline of respiratory microbiota by using invasive techniques to enable reasonable comparisons between sampling methods and between experiments. Although noninvasive sampling (live-bird swabs) resulted in lower 16S ribosomal RNA gene copy numbers compared with invasive sampling, live swabs were able to detect the dominant microbes captured by invasive techniques.
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