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Autologous retinal hair treatment with regard to refractory serous retinal detachment secondary to optic disc abyss: a singular operative method Retinal hair treatment and also optic compact disk opening.
Herein, we report the synthesis of a monocationic μ-nitrido-bridged iron porphycene dimer, a structural analogue of a monocationic μ-nitrido-bridged iron phthalocyanine dimer, which is known to be one of the most potent molecule-based catalysts for methane oxidation. 1H-NMR and single-crystal X-ray structural analyses showed that the porphycene complex includes two Fe(IV) ions, and the structure around the Fe-NFe core is quite similar to that of the monocationic μ-nitrido-bridged iron phthalocyanine dimer. Although methane was oxidized into MeOH, HCHO, and HCOOH in the presence of a silica-supported catalyst of this monocationic μ-nitrido-bridged iron porphycene dimer in an acidic aqueous solution containing excess H2O2, its reactive intermediate was not a high-valence iron-oxo species, as in the case of a monocationic μ-nitrido-bridged iron phthalocyanine dimer, but ˙OH. It is suggested that the high-valent iron-oxo species of the μ-nitrido-bridged iron porphycene dimer was gradually decomposed under these reaction conditions, and the decomposed compound catalyzed a Fenton-type reaction. This result indicates that the stability of the oxo-species is indispensable for achieving high catalytic methane oxidation activity using a μ-nitrido-bridged iron porphyrinoid dimer with an Fe-NFe core as a catalyst.Enhancement of the emission quantum yield and expansion of the emission tunability spectrum are the key aspects of an emitter, which direct the evolution of future generation light harvesting materials. In this regard, small molecular ligand-protected Cu nanoclusters (SLCuNCs) have emerged as prospective candidates. Herein, we report the broadband emission tunability in a SLCuNC system, mediated by in situ ligand replacement. 1,6-Hexanedithiol-protected blue emissive discrete Cu nanoclusters (CuNCs) and red emissive CuNC assemblies have been synthesized in one pot. The red emissive CuNC assemblies were characterized and found to be covalently-linked nanocluster superstructures. The blue emissive CuNC was further converted to a green-yellow emissive CuNC over time by a ligand replacement process, which was mediated by the oxidized form of the reducing agent used for synthesizing the blue emissive nanocluster. Steady-state emission results and fluorescence dynamics studies were used to elucidate that the ligand replacement process not only modulates the emission color but also alters the nature of emission from metal-centered intrinsic to ligand-centered extrinsic emission. Moreover, time-dependent blue to green-yellow emission tunability was demonstrated under optimized reaction conditions.As an acute inflammatory response, sepsis may cause septic shock and multiple organ failure. Rapid and reliable detection of pathogens from blood samples can promote early diagnosis and treatment of sepsis. However, traditional pathogen detection methods rely on bacterial blood culture, which is complex and time-consuming. Although pre-separation of bacteria from blood can help with the identification of pathogens for diagnosis, the required low-velocity fluid environment of most separation techniques greatly limits the processing capacity for blood samples. Here, we present an acoustofluidic device for high-throughput bacterial separation from human blood cells. Our device utilizes a serpentine microfluidic design and standing surface acoustic waves (SSAWs), and separates bacteria from blood cells effectively based on their size difference. The serpentine microstructure allows the operating distance of the acoustic field to be multiplied in a limited chip size via the "spatial multiplexing" and "pressure node matching" of SSAW field. Microscopic observation and flow cytometry analysis shows that the device is helpful in improving the flow rate (2.6 μL min-1 for blood samples; the corresponding velocity is ∼3 cm s-1) without losing separation purity or cell recovery. The serpentine microfluidic design provides a compatible solution for high-throughput separation, which can synergize with other functional designs to improve device performance. Further, its advantages such as low cost, high biocompatibility, label-free separation and ability to integrate with on-chip biosensors are promising for clinical utility in point-of-care diagnostic platforms.A palladium catalysed C-C bond activation of cyclobutanones for the construction of alkenyl and carbonylated indanones has been developed. The in situ generated σ-alkylpalladium intermediate VIA C-C bond cleavage of cyclobutanone could be trapped with N-tosylhydrazones and carbon monoxide, respectively. The reactions were carried out under mild conditions with excellent functional group tolerance.Alba2 is a hyperthermophilic DNA-binding protein, and DNA plays a crucial role in the Alba2 oligomerization process. It is a pity that there is limited research in terms of how DNA affects the conformational change of Alba2 in oligomerization. Herein, we complement the crystal structure of the Ape10b2 (belongs to Alba2)-dsDNA complex (PDB ID 3U6Y) and employ multiple short molecular dynamics (MSMD) simulations to illuminate the influence of DNA on Ape10b2 at four temperatures (300, 343, 363, and 373 K). Our results indicate that DNA could cause the conformational changes of two important regions (loop1 and loop5), which may be beneficial for protein oligomerization. The results of hydrogen bond analysis show that the increasing number of hydrogen bonds between two monomers of Ape10b2 may also be a favorable factor for oligomerization. In addition, Ape10b2 can stabilize DNA by electrostatic interactions with an increase in temperature, and five residues (Arg40, Arg42, Asn43, Asn45, and Arg46) play a stabilizing role during protein binding to DNA. Our findings could help in understanding the favorable factors leading to protein oligomerization, which contributes to enzyme engineering research from an industrial perspective.Long-lasting yet visible-light-driven bacterial inhibition is highly desired for environmental protection and public health maintenance. However, conventional semiconductors such as titanium dioxide (TiO2) are impotent for such antibacterial application due to their low utilization rate for visible light. Herein we report the design of a long-lasting yet visible-light-driven antibacterial agent based on marrying luminescent Au nanoclusters (Au NCs for short) to TiO2 (TiO2-NH2@Au NCs). The as-obtained TiO2-NH2@Au NC antibacterial agent not only possesses superior utilization for visible light due to the participation of Au NCs as a good photosensitizer, but also has excellent separation efficacy of photogenerated carriers, thereby efficiently enhancing the generation of reactive oxygen species (ROS) for killing bacteria. Consequently, the TiO2-NH2@Au NCs display excellent antibacterial activity with good durability against both Gram-positive and Gram-negative bacteria such as Staphylococcus aureus (99.37%) and Escherichia coli (99.92%) under visible-light irradiation (λ ≥ 400 nm). This study is interesting because it provides a paradigm change in the design of long-lasting yet visible-light-driven NC-based antibacterial agents for diversified bactericidal applications.Semiconducting MoS2 layers offer the electrons, reducing conjugated Au(I) to Au atoms, and sebsequently serve as desirable substrates for supporting the interfacial growths of gold nanostructures. Au-covering MoS2 heterostructures perform morphology-varied optical characteristics, and the surface engineering of MoS2 involved by Hg2+ ions results in the differential growths of nanostructures and morphological diversities. Naked-eye colorimetric responses to mercury ions, with a low limit of detection of 1.27 nM, are achieved based on the in situ grown heterostructures.DNA conservation is central to many applications. This leads to an ever-increasing number of samples which are more and more difficult and costly to store or transport. A way to alleviate this problem is to develop procedures for storing samples at room temperature while maintaining their stability. A variety of commercial systems have been proposed but they fail to completely protect DNA from deleterious factors, mainly water. On the other side, Imagene company has developed a procedure for long-term conservation of biospecimen at room temperature based on the confinement of the samples under an anhydrous and anoxic atmosphere maintained inside hermetic capsules. The procedure has been validated by us and others for purified RNA, and for DNA in buffy coat or white blood cells lysates, but a precise determination of purified DNA stability is still lacking. We used the Arrhenius law to determine the DNA degradation rate at room temperature. We found that extrapolation to 25°C gave a degradation rate constant equivalent to about 1 cut/century/100 000 nucleotides, a stability several orders of magnitude larger than the current commercialized processes. Such a stability is fundamental for many applications such as the preservation of very large DNA molecules (particularly interesting in the context of genome sequencing) or oligonucleotides for DNA data storage. Capsules are also well suited for this latter application because of their high capacity. One can calculate that the 64 zettabytes of data produced in 2020 could be stored, standalone, for centuries, in about 20 kg of capsules.
Biological sex has a paramount influence on the pathophysiology of diseases, and thus on clinical presentation. In this study, we provide a comprehensive analysis of sex-specific differences in patients with myocarditis.

Patients with myocarditis who were admitted to our study center in the time-period of 2009 to 2019 were retrospectively enrolled in this study. Clinical data, laboratory parameters and measurements from transthoracic echocardiography were extracted from hospital records. Follow-up was acquired for 2 years after admission.

224 patients with myocarditis were enrolled in this study. Of these, 78% were males and 22% females. Female patients were older (median 50 years vs. 35 years, p<0.0001), had a higher prevalence of respiratory tract infections and less frequently ST-segment elevations on ECG (28% vs. 59%, p= 0.003). Furthermore, C-reactive protein was lower in females (median 0.60 mg/dl vs. 3.90 mg/dl, p<0.0001), but showed a less pronounced decrease within three days when compareding of its pathophysiology.
Hypothalamic injury causes several complicated neuroendocrine-associated disorders, such as water-electrolyte imbalance, obesity, and hypopituitarism. Among these, central diabetes insipidus (CDI), characterized by polyuria, polydipsia, low urine specific gravity, and deficiency of arginine vasopressin contents, is a typical complication after hypothalamic injury.

CDI was induced by hypothalamic pituitary stalk injury in male animals. Behavioral parameters and blood sample were collected to evaluate the characteristics of body fluid metabolism imbalance. 2-DG in vivo The brains were harvested for high-throughput RNA sequencing and immunostaining to identify pathophysiological changes in corresponding hypothalamic nuclei.

Based on transcriptomic analysis, we demonstrated the upregulation of the Atf3/c-Jun axis and identified Lgals3, a microglial activation related gene, as the most significant target gene in response to the body fluid imbalance in CDI. Furthermore, we found that the microglia possessed elevated phagocytic ability, which could promote the elimination of arginine vasopressin neurons after hypothalamic injury.
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