Notes

VprBP guides epigenetic gene silencing via histone H2A phosphorylation within colon cancer.
Hypertrophic scar (HS) is a frequently diagnosed skin disease that is difficult to treat. HS is usually associated with itching and pain and causes both physical and psychological issues. In this study, a safe, convenient, and efficient therapy for HS is developed. Carboxymethyl chitosan (CMCH) and Bletilla striata polysaccharide (BSP) are used to prepare microneedles (MN) via a micro-molding method. Hydroxypropyl β-cyclodextrin (HP-β-CD) is used to encapsulate triamcinolone acetonide (TA) and the obtained inclusion is co-loaded with verapamil (VRP) to MN. The MN is then attached to an Ethyl cellulose (EC) base layer to obtain a MN patch. The MN patch has uniform needles, sufficient mechanical strength, good penetration and dissolution in skin, and low cytotoxicity. It also significantly decreases the thickness of HS, and hydroxyproline (HYP) and transforming growth factor-beta 1 (TGF-β1) expression in HS, improves collagen fiber arrangement, and reduces dermis congestion and hyperplasia.Quercetin-chitosan (QCS) polysaccharide was synthesized via non-radical reaction using L-valine-quercetin as the precursor. QCS was systematically characterized and demonstrated amphiphilic properties with self-assembling ability. this website In-vitro activity studies confirmed that quercetin grafting does not diminish but rather increases antimicrobial activity of the original chitosan (CS) and provided the modified polysaccharide with antioxidative properties. QCS applied as a coating on fresh-cut fruit reduced microbial spoilage and oxidative browning of coated melon and apple, respectively. Notably, QCS-based coatings prevented moisture loss, a major problem with fresh produce (2%, 12% and 18% moisture loss for the QCS-coated, CS-coated and uncoated fruit, respectively). The prepared QCS polysaccharide provides advanced bioactivity and does not involve radical reactions during its synthesis, therefore, it has good potential for use as a nature-sourced biocompatible active material for foods and other safety-sensitive applications.Studies on the effect of sulfated polysaccharides from seaweed on bone regeneration have increased in recent years. However, there is no consensus on how to use them and their real effectiveness in that process. Thereby, we carried out a systematic review to answer the question "Do the sulfated polysaccharides from seaweeds promote osteogenesis?". Searches were performed in Pubmed, Scopus, and Web of Knowledge databases. A total of 599 articles were selected, resulting in 14 eligible studies. Results showed that the sulfated polysaccharides from seaweeds increase the osteogenic markers evaluated. Nevertheless, due to the lack of standardization on protocols used, the results should be cautiously interpreted. In addition, studies using animal models are still scarce, and the results with cellular models cannot always be extrapolated to systems that are more complex. Despite the study limitations, the use of sulfated polysaccharides appears to promote in vitro osteogenesis and enhance bone regeneration.Cellulose nanomaterials have received significant interest due to their superior physicochemical properties and biocompatibility. The nanomaterials-based hydrogel patches are widely explored for skin regeneration. However, the injectability and adhesiveness of the hydrogels are crucial challenges for tissue engineering applications. To overcome these, we synthesized an injectable and adhesive hydrogel of spherical nanocellulose (s-NC) reinforced carboxymethyl chitosan for rapid skin regeneration. The s-NC exhibited improved cellular activity than cellulose nanocrystals. The hydrogels exhibited adhesive and injectability potentials and were molded in the desired configurations. An enhanced conductivity was observed in s-NC added hydrogels than the pure polymer hydrogel. The skin regeneration potential of the hydrogel scaffolds was also examined in the rats using the wound healing model. The composite scaffolds also showed improved antibacterial potential. Taken together, the developed hydrogels have the potential and can be explored as a promising biomaterial for enhanced skin regeneration applications.The application of flexible multifunctional sensors based on conductive hydrogels in human health detection has been widely studied. Herein, a facile one-pot method is proposed to prepare ionic conductive hydrogels by dissolving polyvinyl alcohol (PVA), cellulose nanofiber (CNF), and aluminum chloride hexahydrate (AlCl3·6H2O) in a dimethyl sulfoxide (DMSO)/water binary solvent. The resulting ionically-conductive organohydrogels have high stretchability (up to 696%), fast response (130 ms), wide operating temperature (-50 °C to 50 °C), and long-term stability (30 days). The hydrogel sensor exhibits excellent signal sensing capability (human motion and sound detection signals) and cycling stability (1000 cycles) under extreme temperature and long-term storage conditions. Notably, the organohydrogel displays high sensitivity to both compressive deformation and temperature, resulting in multifunctional sensing performance. This work provides a viable approach for the long-term use of hydrogels as wearable devices in extreme environments and daily life.Owing to its purity and exceptional mechanical performance, bacterial nanocellulose (BNC) is well suited for tissue engineering applications. BNC assembles as a network that features similarities with the extracellular matrix (ECM) while exhibiting excellent integrity in the wet state, suitable for suturing and sterilization. The development of complex 3D forms is shown by taking advantage of the aerobic process involved in the biogenesis of BNC at the air/culture medium interphase. Hence, solid supports are used to guide the formation of BNC biofilms that easily form auxetic structures. Such biomaterials are demonstrated as implantable meshes with prescribed opening size and infill density. The measured mechanical strength is easily adjustable (48-456 MPa tensile strength) while ensuring shape stability (>87% shape retention after 100 burst loading/unloading cycles). We further study the cytotoxicity, monocyte/macrophage pro-inflammatory activation, and phenotype to demonstrate the prospective use of BNC as supportive implants with long-term comfort and minimal biomaterial fatigue.In this work, effects of ascorbic acid (AH2) treatment on the viscosity and structural properties of okra pectic polysaccharide (OPP) and its mechanism were investigated. Results showed that AH2 could decrease the apparent viscosity of OPP, and the reducing ability was promoted by high temperature and the addition of Fe2+, Cu2+ or H2O2. The molecular weight was significantly decreased with increasing AH2 concentration, but it had little effects on the monosaccharide composition, glycosidic linkages, infrared characteristics, and morphology of OPP after AH2 incubation. Hydroxyl radicals were generated during the incubation, which can be activated by introducing Fe2+, Cu2+ or H2O2. In summary, the viscosity reduction of OPP induced by AH2 was related to the formation of hydroxyl radicals. The present study provides some recommendations for the application of OPP in food and other products containing AH2.We prepared chitosan (CS) based multifunctional hydrogels using oxidized succinoglycan (OSG) with a semi-dissolving acidified sol-gel transition method. OSG cross-linked CS hydrogels (OSG/CS) was prepared by aldehyde-amine Schiff-base reaction. OSG/CS increased not only thermal stability but also improved mechanical strength by 5.75 times. Through the tensile and strain sweep test, OSG/CS showed excellent self-healing properties by 98.82% and 99.89%, respectively. It showed the high compressive stress of 173 kPa at 60% strain, the adhesive strength of 2763 kPa, and the antibacterial effect of 90%. Furthermore, OSG/CS showed a pH-controlled drug release pattern, where a change of pH from 7.4 to 2.0 accelerated for 5-fluorouracil release from 60% to 90%. WST-8 assay demonstrated that OSG/CS maintained 97.30% cell viability and 98.84% cell proliferation after 7 days, indicating the potential as biocompatible hydrogel materials such as wound healing, tissue engineering and drug release systems.An environmental-friendly lipase induced highly hydrophobic NFC film was fabricated through lipase induced dimethyl adipate (DA) esterification followed by silver nanowires (AgNWs) coating for strain sensor application. Due to the lipase activation, the substitution degree (DSNMR) of 0.18 was achieved, which was three times higher than that of the control sample (without lipase treatment of NFC-DA). As a result, the water contact angle (WCA) of lipase induced adipated-NFC film was reached to 105 ± 3° from 50 ± 2.3° of NFC-DA. In addition, the cellulose structure and performance were well maintained after lipase induced esterification, confirmed by AFM, SEM, TG/DTG, and XRD analysis. After AgNWs coating and annealing, the hydrophobic NFC film-based strain sensor exhibited excellent sensitivity towards human motion, such as finger/wrist movement in real-time, even under wet conditions. Overall, a highly hydrophobic NFC film-based strain sensor was fabricated, which has promising application in wearable devices for human motion monitoring.Bone Morphogenetic Protein (BMP-2) is an osteoinductive growth factor clinically used for bone regeneration. Tuneable sustained strategies for BMP-2 delivery are intensely developed to avoid severe complications related to supraphysiological doses applied. To address this issue, we investigated the ability of the bacterial exopolysaccharide (EPS) called Infernan produced by the deep-sea hydrothermal vent bacterium Alteromonas infernus, exhibiting both glycosaminoglycan-mimetic and physical gelling properties, to efficiently bind and release the bioactive BMP-2. Two delivery systems were designed based on BMP-2 retention in either single or complex EPS-based microgels, both manufactured using a microfluidic approach. BMP-2 release kinetics were highly influenced by the ionic strength, affecting both microgel stability and growth factor/EPS binding, appearing essential for BMP-2 bioactivity. The osteogenic activity of human bone-marrow derived mesenchymal stem cells studied in vitro emphasized that Infernan microgels constitute a promising platform for BMP-2 delivery for further in vivo bone repair.This paper proposes a method to modify high-amylose maize starch (HAMS) with lauric acid in an ethanol solvothermal system to prepare a cold-water swelling esterified starch (M-HAMS). Using M-HAMS as a granule stabilizer, oil-in-water Pickering emulsions were prepared, and factors affecting the oxidation stability of emulsion, such as granule content, oil-water ratio, pH value, and NaCl concentration, were studied. Atomic force microscopy (AFM) analysis showed that M-HAMS granules swell into dense molecular chains liking a three-dimensional network barrier in the emulsion, which increases the viscosity of continuous phase and prevents the oil droplets from contacting the pro-oxidant in the water phase, thus reducing the peroxide value. At the same time, the influence of changes in pH value and NaCl concentration on the viscosity and stability of emulsion is weakened. Therefore, the preparation of M-HAMS granule by ethanol solvothermal is a simple and effective method.
Read More: https://www.selleckchem.com/products/wy-14643-pirinixic-acid.html