Notes

Affect associated with heat in biomass production of clones of Atriplex halimus.
Chitinaceous organisms have been found to ingest microplastic; however, a standardised, validated, and time- and cost-efficient method for dissolving these organisms without affecting microplastic particles is still required. This study tested four protocols for dissolving organisms with a chitin exoskeleton 1) potassium hydroxide (KOH) + chitinase, 2) Creon® + chitinase, 3) hydrogen peroxide (H2O2) + chitinase, and, 4) Nitric Acid (HNO3) + hydrogen peroxide (H2O2). The effects on microplastics composed of eight different polymers were also tested. The use of H2O2 followed by chitinase was found to be a highly efficient method. The three other protocols either did not digest the chitin sufficiently or negatively affected the tested polymers. A recovery test using microplastic fibres, beads and tyre particles revealed high recovery rates of 0.85, 0.89 and 1 respectively. This further supported the applicability of the H2O2 and chitinase (protocol 3) for dissolving chitinaceous organisms. Thus, we recommend that future investigations of microplastic (0.05 μm-5000 μm) in chitinaceous organisms (0.3 cm-5 cm) utilise the here presented methodology. This represents an important component of the ongoing validation and harmonization of methodological approaches that are urgently needed for the advancement of microplastic assessments globally.Sertraline hydrochloride (Ser-HCl) is an effective and commonly used antidepressant drug, which is also frequently detected in aquatic environments. Our previous research showed that Ser-HCl changes the community composition of aquatic microbiome, but the understanding of the expression of functional pathways in microbial communities is still incomplete; to address this knowledge gap, we used meta-transcriptomics analysis to evaluate the toxicity of Ser-HCl to natural aquatic microbial communities cultured in laboratory microcosms. Meta-transcriptomic results show that a 15-day exposure to 50 μg/L Ser-HCl significantly changed the functional expression activity of aquatic microbial communities. Pathways related to lipid metabolism, energy metabolism, membrane transport function, and genetic information processing in the aquatic microbial community were severely inhibited under Ser-HCl treatment, but metabolism of cofactors and vitamins to alleviate biological toxicity after Ser-HCl exposure was enhanced. Our study thus reveals details of the effects of sertraline on the functioning of aquatic microbiome. Due to the extensive use of Ser-HCl and its strong biological activity, it should not continue to be an overlooked pollutant. Therefore, more attention should be paid to the negative effects of such biologically active drugs on the expression of aquatic microbiome.The study objective was to derive a susceptibility model for shallow landslides that could include process-related non-stationary variables, to be adaptable to climate changes. We selected the territory of the Mont-Emilius and Mont-Cervin Mountain Communities (northern Italy) as the study area. To define summary variables related to landslide predisposing and triggering processes, we investigated the relationships between landslide occurrences and intense rainfall and snowmelt events (period 1991-2020). For landslide susceptibility mapping, we set up a Generalized Additive Model. We defined a reference model through variable penalization (relief, NDVI, land cover and geology predictors). Similarly, we optimized a model including the climate variables, checking their smooth functions to ensure physical plausibility. learn more Finally, we validated the optimized model through a k-fold cross-validation and performed an evaluation based on contingency tables, area under the receiver operating characteristic curve (AUROC) a analysis. Not only do the variables improve the model performance, but they make it adaptable to map the future evolution of landslide susceptibility including climate changes.Herbivory of insular plant communities by introduced animals has been widely studied for decades. Though their diet mainly includes palatable and highly nutritive species, goats will also eat plants that are toxic to other animals. Thus, severe affection of toxic species may indicate high herbivore pressure or a low quality of vegetative food. To evaluate whether herbivory damage to low-palatability shrubs could give us information about feral goat pressure on vegetation, we assessed the predation impact of feral goats on the shrub Euphorbia dendroides (Euphorbiaceae) on Mallorca Island (Spain). We aimed to investigate whether goats consume juvenile E. dendroides and affect their population structure and determine if the plants increase the concentrations of toxic compounds as an adaptation to herbivory. Overall, two experimental plots and analysis of eleven natural populations indicated E. dendroides is affected by ungulates and that the population structure change with the presence of feral goats. Euphorbia dendroides could be used as an ecological indicator to determine the extent of ungulate damage to vegetation or indicate poor food availability, and thus inform the maintenance of optimal animal populations. Depending on the management objective for the territory, E. dendroides could be used as an ecological indicator to determine the extent of ungulate damage to vegetation or indicate poor food availability for animals, and thus inform the maintenance of optimal animal populations.Fipronil (FP) is widely used as a highly effective insecticide worldwide, thereby raising concern about environmental contamination and risk for human health. However, data on the occurrence of FP and its transformation products (FPs) in human blood and urine are limited. In this study, 39 pairs of serum, plasma, blood cells (BCs), and urine samples were collected from adults in Wuhan, central China (2020), in order to characterize the concentration profiles of FPs in different matrices. FPs were also determined in serum samples (n = 226, including 57, 56, 56, and 57 samples for Wuhan, Huangshi, Nanjing, and Zhenjiang, respectively) collected from four cities of China (2015) to characterize the exposure levels of FPs among the general population and potential spatial variations. Fipronil sulfone (FP-SFO) was 100% detectable in blood samples, and it was the predominant metabolite (accounting for 86-95% of the cumulative concentrations of FPs [ΣFPs]), with the median concentrations (ng/mL) of 0.17, 0.16, and 0.
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