Notes

Stone outcrops decrease temperature-induced anxiety regarding exotic conifer through decoupling localized weather within the semiarid setting.
To further clarify the relationship between environmental regulation and green technology innovation and discuss how environmental regulation affects green technology innovation through import trade, this paper analyzes the impacts of environmental regulation and import trade on green technology innovation and the transmission effect of import trade based on panel data for 30 provinces in China for 2008 to 2017. The results show that (1) environmental regulation first plays a role in promoting green technology innovation and then restrains it, and import trade can significantly promote green technology innovation; (2) under the constraints of stronger environmental regulations, import trade has a significantly positive effect on green technology innovation; and (3) environmental regulation can further enhance the technology spillover effects of import trade in regions with high absorptive capacity and regions with high levels of R&D investment. This paper analyzes the impact of environmental regulation on green technology innovation from the perspective of import trade and makes up for the deficiencies of existing research. It also lays a foundation for scholars to study the relationship between environmental regulation and green technology innovation in the midst of heterogeneous government regulation capabilities and industries in the future.In recent years, the occurrence of floods is one of the most important challenges facing in Hamadan city. In the absence/inefficiency of urban drainage systems, rainwater harvesting (RWH) systems as low-impact development (LID) methods can be considered as a measure to reduce the floods. In this study, three scenarios concerning the RWH from the roof surfaces are studied to evaluate the type of the harvested water on reducing flooding. In the first scenario, which indicates the current situation in the studied area, it is indicated that there is no harvest of the roof surfaces in the studied area. The second scenario is about the use of water harvested from the roof surfaces for household purposes. The third scenario also refers to the use of harvested water for irrigation of gardens. The simulation results of these three scenarios using the Soil Conservation Service (SCS) method in the Hydrologic Modeling System (HEC-HMS) model reveal that if the second scenario is implemented, the runoff volume decreases from 28 to 12% for the return period from 2 to 100 years. However, in the third scenario, this reduction in runoff volume will be 48 and 27% for return periods of 2 to 100 years, respectively. Therefore, the results of this study indicate that the use of harvested water can also affect the reduction on runoff volume.Recently, anthropogenic alterations have had severe and negative impacts on the terrestrial and aquatic species and environments. To conserve species that have a small and limited habitat, it is necessary to focus on fine-scale population structure and its effects on persistence. The deepbodied bitterling Acheilognathus longipinnis is an endangered freshwater fish that occupies ponds scattered in lateral bars in the Kiso River. In this study, we conducted multi-locus microsatellite DNA analysis to evaluate both fine-scale population structure and genetic diversity, in order to conserve A. longipinnis. The smaller number of loci deviating from the Hardy-Weinberg equilibrium in ponds scattered in individual lateral bars compared to the whole river system suggests that A. longipinnis forms a local breeding population in units of ponds. The population was roughly split between the river banks and the local population located in ponds in the mid-channel bar showed intermediate relationships with the river bank populations. Gene flow between local populations was not always homogeneous and was not influenced by geographical distances between local populations or the direction of river flow. The dispersal of A. longipinnis across both river bank sides may be constrained and is probably affected by the ecological characteristics of A. longipinnis and the hydrological regimes. Consequently, A. longipinnis in the Kiso River is maintained as a complex of multiple local populations with appropriate gene flow among them. To conserve A. read more longipinnis, both the persistence of the unstable ponds and moderate genetic exchanges by individual migration are required.
Develop a Cell Surface Display system in Saccharomyces cerevisiae, based on the construction of an expression cassette for pYES2 plasmid.

The construction of an expression cassette containing the α-factor signal peptide and the C-terminal portion of the α-agglutinin protein was made and its sequence inserted into a plasmid named pYES2/gDαAgglutinin. The construction allows surface display of bovine herpesvirus type 5 (BoHV-5) glycoprotein D (gD) on S. cerevisiae BY4741 strain. Recombinant protein expression was confirmed by dot blot, and indirect immunofluorescence using monoclonal anti-histidine antibodies and polyclonal antibodies from mice experimentally vaccinated with a recombinant gD.

These results demonstrate that the approach and plasmid used represent not only an effective system for immobilizing proteins on the yeast cell surface, as well as a platform for immunobiologicals development.
These results demonstrate that the approach and plasmid used represent not only an effective system for immobilizing proteins on the yeast cell surface, as well as a platform for immunobiologicals development.
An attractive cell source for stem cell-based therapy are WJ-MSCs. Hence, tracking WJ-MSCs using non-invasive imaging procedures (such as MRI) and contrast agents (Zn0.5Ni0.5Fe2O4, NFNPs) are required to evaluate cell distribution, migration, and differentiation.

Results showed that the bare and dextrin-coated NFNPs were internalized inside the WJ-MSCs and had no effect on the cell viability, proliferation, apoptosis, karyotyping, and morphology of WJ-MSCs up to 125µg/mL. Besides, treated WJ-MSCs were differentiated into osteo/adipocyte-like cells. The expression of RUNX 2, SPP 1 (P < 0.05), and OCN (P > 0.05) genes in the WJ-MSCs treated with dextrin-coated NFNPs was higher than the untreated WJ-MSCs; and the expression of CFD, LPL, and PPAR-γ genes was reduced in WJ-MSCs treated with both NFNPs in comparison with the untreated WJ-MSCs (P > 0.05).

Overall, results showed that dextrin-coated NFNPs had no adverse effect on the cellular characteristics, proliferation, and differentiation of WJ-MSCs, and suggesting their potential clinical efficacy.
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