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Manufactured antimicrobial peptides handle Penicillium digitatum infection within orange fruit.
Time-dependent AFM creep deformation for both the wild type and ctpA mutant was indicative of a viscoelastic cell envelope, with best fit to the four-element Burgers model and generating values for viscoelastic parameters k1, k2, η1, and η2; (4) Conclusions The viscoelastic response of the ctpA mutant is consistent with both its compromised outer membrane (TEM) and fortified peptidoglycan layer (HPLC/MS).How photosynthetic-related leaf traits of non-nitrogen (N)-fixing pioneer species respond to extreme habitat conditions of primary succession is still not well-elucidated, especially in volcanically N-deplete habitats. The effect of N-deplete soil on photosynthetic-related leaf traits can provide a basis for predicting how plants adjust their strategies to adapt to such habitats. To examine the responses of leaf traits to extreme conditions, we investigated Miscanthus condensatus (a non-N-fixing C4 pioneer grass) which grows on a volcanically devastated area on Miyake-jima Island, Japan, in which the volcanic ash has been deposited for 17-18 years since the 2000-year eruption. Leaf N content (Narea), light-saturated photosynthetic rate (Amax), and photosynthetic N use efficiency (PNUE) in three contrasting study sites bare land (BL), shrub land gap (SLG), and shrub land under canopy (SLUC) were determined. Results indicated that compared to previous studies and internal comparison of Miyake Island, M. condensatus in BL was able to maintain a relatively high Amax, Narea and PNUE. The higher Amax was in part a result of the higher PNUE. This is a characteristic necessary for its successful growth in N-deplete soils. These results suggest that M. condensatus has photosynthetic-related advantages for adaptation to volcanically N-deplete habitats.Rickettsia typhi and Bartonella henselae are the causative agents of murine typhus and cat-scratch disease, respectively. A small-scale survey (N = 202) was conducted in the Attica region, Greece, for determining the prevalence rates of IgG antibodies against B. henselae and R. typhi by indirect fluorescence antibody test. IgG against B. henselae and R. typhi were present in 17.8% (36/202) and 4.5% (9/202) of the participants, respectively; co-occurring IgG against both B. henselae and R. typhi were detected in 3.5% (7/202), whereas only anti-B. henselae IgG in 14.3% (29/202), and only anti-R. typhi IgG in 1.0% (2/202). Titres 1/64, 1/128, 1/256, and 1/512, of anti-B. henselae IgG were identified in 6.4%, 4.5%, 4.5%, and 2.4%, whereas titres 1/40 and 1/80 of anti-R. typhi IgG were detected in 4.0%, and 0.5%, respectively. A positive association of anti-B. henselae IgG prevalence with a coastal area featuring a major seaport (p = 0.009) and with younger age (p = 0.046) was identified. The findings of this survey raise concern for exposure of the population of Attica to B. henselae and R. typhi, which should be considered in the differential diagnosis when compatible symptoms are present. Our results also suggest that seaports may represent high-risk areas for exposure to Bartonella spp.We studied the influence of aging temperature on microstructure and mechanical properties in an ultra-low carbon Cu bearing steel in the present study. During the aging process, a continuous recovery of matrix associated with formation and growth of Cu precipitates could be observed during aging processes, exerting significant effects on the mechanical properties of the steel. At aging temperature below 600 °C, the mechanical properties were dominated by the precipitation strengthening effect, leading to excessive matrix strengthening and poor low-temperature toughness. Conversely, steel aged at temperatures above 650 °C exhibited an extraordinary improvement in toughness at the expense of strength, which can be attributed to the synergistic effects of softening matrix, coarsened Cu precipitates and formation of reverted austenite. After aging at 650 °C, reverted austenite formed at the lath boundaries. Increasing the aging temperature to 700 °C lowered the thermal stability of reverted austenite, consequently, the reverted austenite was partially transformed to fresh martensite. After aging at 650 °C for 0.5 h, the mechanical properties were optimized as follows-yield strength = 854 MPa, tensile strength = 990 MPa, elongation = 19.8% and Charpy impact energy = 132 J at -80 °C.Steel bar corrosion caused by chloride was one of the main forms of concrete deterioration. The promotion of chloride binding capacity of cementitious materials would hinder the chloride transport to the surface of steel bar, thereby alleviating the corrosion and mitigating the deterioration. A comparative study on binding capacity of chloride in cement-fly ash system (C-FA) and cement-ground granulated blast furnace slag system (C-GGBS) with diethanol-isopropanolamine (DEIPA) was investigated in this study. Chloride ions was introduced by adding NaCl in paste, and the chloride binding capacity of the paste samples at 7 d and 60 d was examined. The hydration process was discussed via the testing of hydration heat and compressive strength. The hydrates in hardened paste was characterized by X-ray Diffractometry (XRD), Thermo Gravimetric Analysis (TGA), and Scanning Electron Microscope (SEM). The effect of DEIPA on dissolution of aluminate phase and compressive strength was discussed as well. These results showed that DEIPA could facilitate the hydration of C-FA and C-GGBS system, and the promotion effect was higher in C-FA than that in C-GGBS. DEIPA also increased the binding capacity of chloride in C-FA and C-GGBS systems. One reason was the increased chemical binding, because DEIPA facilitated the dissolution of aluminate to benefit the formation of Friedel's salt. Other reasons were the increased physical binding and migration resistance. By contrast, DEIPA presented greater ability to increase chloride binding capacity in C-FA system, because DEIPA showed stronger ability to expedite the dissolution of aluminate of FA than that of GGBS, which benefited the formation of FS, thereby promoting the chemical binding. Such results would give deep insight into using DEIPA as an additive in cement-based materials.Multiple co-localized paralogs of genes in Polypedilum vanderplanki's genome have strong transcriptional response to dehydration and considered to be a part of adaptation machinery at the larvae stage. One group of such genes represented by L-isoaspartate O-methyltransferases (PIMT). In order to highlight specific role of PIMT paralogization in desiccation tolerance of the larvae we annotated and compared S-adenosylmethionine (SAM) dependent methyltransferases of four insect species. From another side we applied co-expression analysis in desiccation/rehydration time course and showed that PIMT coding genes could be separated into five clusters by expression profile. We found that among Polypedilum vanderplanki's PIMTs only PIMT1 and PIMT2 have enzymatic activity in normal physiological conditions. From in silico analysis of the protein structures we found two highly variable regions outside of the active center, but also amino acid substitutions which may affect SAM stabilization. Overall, in this study we demonstrated features of Polypedilum vanderplanki's PIMT coding paralogs related to different roles in desiccation tolerance of the larvae. Our results also suggest a role of different SAM-methyltransferases in the adaptation, including GSMT, JHAMT, and candidates from other classes, which could be considered in future studies.With the blooming of oil and gas industries, oily saline wastewater treatment becomes a viable option to resolve the oily water disposal issue and to provide a source of water for beneficial use. Reverse osmosis (RO) has been touted as a promising technology for oily saline wastewater treatment. However, one great challenge of RO membrane is fouling phenomena, which is caused by the presence of hydrocarbon contents in the oily saline wastewater. This study focuses on the fabrication of antifouling RO membrane for accomplishing simultaneous separation of salt and oil. Selleckchem WST-8 Thin film nanocomposite (TFN) RO membrane was formed by the layer by layer (LbL) assembly of positively charged TNS (pTNS) and negatively charged TNS (nTNS) on the surface of thin film composite (TFC) membrane. The unique features, rendered by hydrophilic TNS bilayer assembled on TFC membrane in the formation of a hydration layer to enhance the fouling resistance by high concentration oily saline water while maintaining the salt rejection, were discussed in this study. The characterization findings revealed that the surface properties of membrane were improved in terms of surface hydrophilicity, surface roughness, and polyamide(PA) cross-linking. The TFC RO membrane coated with 2-bilayer of TNS achieved >99% and >98% for oil and salt rejection, respectively. During the long-term study, the 2TNS-PA TFN membrane outperformed the pristine TFC membrane by exhibiting high permeability and much lower fouling propensity for low to high concentration of oily saline water concentration (1000 ppm, 5000 ppm and 10,000 ppm) over a 960 min operation. Meanwhile, the average permeability of uncoated TFC membrane could only be recovered by 95.7%, 89.1% and 82.9% for 1000 ppm, 5000 ppm and 10,000 ppm of the oily saline feedwater, respectively. The 2TNS-PA TFN membrane achieved almost 100% flux recovery for three cycles by hydraulic washing.Gastrointestinal tract microbiota plays a key role in the regulation of the pathogenesis of several gastrointestinal diseases. In particular, the viral fraction, composed essentially of bacteriophages, influences homeostasis by exerting a selective pressure on the bacterial communities living in the tract. Gastrointestinal inflammatory diseases are mainly induced by bacteria, and have risen due to the emergence of antibiotic resistant strains. In the lack of effective treatments, phage therapy has been proposed as a clinical alternative to restore intestinal eubiosis, thanks to its immunomodulatory and bactericidal effect against bacterial pathogens, such as Clostridioides difficile in ulcerative colitis and invasive adherent Escherichia coli in Crohn's disease. In addition, genetically modified temperate phages could be used to suppress the transcription of bacterial virulence factors. In this review, we will highlight the latest advances in research in the field, as well as the clinical trials based on phage therapy in the area of gastroenterology.A CMOS (Complementary metal-oxide-semiconductor) Hall sensor with low power consumption and simple structure is introduced. The tiny magnetic signal from Hall device could be detected by a high-resolution delta-sigma ADC in presence of offset and flickering noise. Also, the offset as well as the flickering noise are effectively suppressed by the current spinning technique combined with double sampling switches of the ADC. The double sampling scheme of the ADC reduces the operating frequency and helps to reduce the power consumption. The prototype Hall sensor is fabricated in a 0.18-µm CMOS process, and the measurement shows detection range of ±150 mT and sensitivity of 110 µV/mT. The size of active area is 0.7 mm2, and the total power consumption is 4.9 mW. The proposed system is advantageous not only for low power consumption, but also for small sensor size due to its simplicity.
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