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Rhodopsin, a prototypical G-protein-coupled receptor, is responsible for scoptic vision at low-light levels. Although rhodopsin's photoactivation cascade is well understood, it remains unclear how lipid and zinc binding to the receptor are coupled. Using native mass spectrometry, we developed a novel data analysis strategy to deconvolve zinc and lipid bound to the proteoforms of rhodopsin and investigated the allosteric interaction between lipids and zinc binding. We discovered that phosphatidylcholine bound to rhodopsin with a greater affinity than phosphatidylserine or phosphatidylethanolamine, and that binding of all lipids was influenced by zinc but with different effects. In contrast, zinc binding was relatively unperturbed by lipids. Overall, these data reveal that lipid binding can be strongly and differentially influenced by metal ions.Wealth accumulation is a key dimension of ethno-racial stratification, and, among immigrants, an important indicator of incorporation. Dramatically low assets among immigrant Latinos is thus a pressing concern, necessitating a better understanding of the social forces that shape wealth assimilation. Drawing on a survey of Latino immigrants in Durham, NC, I argue for the importance of a transnational perspective on wealth for immigrant populations. Nationally representative surveys designed to assess inequality among the general population generally lack information on wealth held abroad, which accounts for the lion's share of assets held by immigrants in our sample. Likewise, these data sources rarely have information on factors salient to immigrants, particularly legal status and informal employment. Finally, I show that the socio-demographic characteristics central to life-cycle wealth models operate in different ways for U.S. and foreign assets, and for men and women. Fluorescein-5-isothiocyanate cost For instance, while household earnings and duration of Durham residence are associated with greater U.S. assets among Durham's Latino migrants, they fail to predict wealth held abroad. Likewise, low educational attainment and informal employment are associated with lower U.S., but not foreign, wealth. link2 Instead, the key predictors of wealth abroad relate to family structure. I further document structural barriers to immigrant Latino wealth accumulation, such as employment marginality and lack of access to mainstream financial institutions.Though employment outside of regular daytime business hours has remained high since the 1990s, trends in nonstandard employment schedules over the life course and across households remain under-examined. The consequences of nonstandard scheduling extend to workers, their spouse, and children, urging greater attention to the distribution of nonstandard schedules at the couple-level. link3 Using all three waves of the National Survey of Families and Households, this article examines the prevalence, persistence and sociodemographic patterns of rotating and night employment at the couple-level, following 913 married couples in the United States as they aged from the late 1980s to early 2000s. Though aging reduced the likelihood that couples had one or both spouses working nonstandard hours, roughly one-third of couples with nonstandard scheduling continued to experience nonstandard schedules during the subsequent observation period. Nonstandard schedules were stratified by education and race/ethnicity. This stratification persisted as couples aged, even after controlling for prior work schedules. Findings suggest that disadvantaged couples remain disproportionately exposed to schedules associated with negative outcomes for family well-being across the life course.When QGIS 3.0 was released in 2018, it added support for 3D visualisation. At the same time, CityJSON has been developing as an easy-to-use JavaScript Object Notation (JSON) encoding for 3D city models using the CityGML 2.0 data model. Together, this opened the possibility to support semantic 3D city models in the popular open-source GIS software for the first time. In order to add support for 3D city models in QGIS, we have developed a plugin that enables CityJSON datasets to be loaded. The plugin parses a CityJSON file and analyses its tree structure to identify all city objects. Then, the geometry and attributes of every city object are transformed into QGIS features and divided into layers according to user preferences. CityJSON parsing was proven to be straightforward and consistent when tested against several open datasets. One of the biggest challenges we faced, though, was mapping CityJSON's hierarchical data structure to the relational model of QGIS. We undertook this issue by providing various methods on how geometries from the model are loaded as QGIS features. We intend to use the plugin for educational purposes in our university and we believe it can be proven a worthy tool for researchers and practitioners.Interaction of vacancies with grain boundaries (GBs) is involved in many processes occurring in materials, including radiation damage healing, diffusional creep, and solid-state sintering. We analyze a model describing a set of processes occurring at a GB in the presence of a non-equilibrium, non-homogeneous vacancy concentration. Such processes include vacancy diffusion toward, away from, and across the GB, vacancy generation and absorption at the GB, and GB migration. Numerical calculations within this model reveal that the coupling among the different processes gives rise to interesting phenomena, such as vacancy-driven GB motion and accelerated vacancy generation/absorption due to GB motion. The key combinations of the model parameters that control the kinetic regimes of the vacancy-GB interactions are identified via a linear stability analysis. Possible applications and extensions of the model are discussed.Sexual harassment is a common experience among college students, and its perpetration may be associated with deficits in perspective taking -- a form of empathy -- and institutional factors such as climates. This investigation compared reported outcomes after sexual harassment and perceptions of institutional support between perpetrators and victims of sexual harassment. A total of 579 students responding to a campus climate survey indicated that they were victims or perpetrators of sexual harassment in the past seven months. Perpetrators of sexual harassment perceived that their victims experienced far fewer negative outcomes than victims reported actually experiencing. Additionally, victims of sexual harassment had significantly worse perceptions of institutional support than did perpetrators of sexual harassment. This information can be used to inform primary and secondary prevention methods utilized by universities.
Utilization of wheat germ and wheat germ oil is limited due to high enzymatic activity and the presence of unsaturated fatty acids, which require stabilization techniques to overcome this problem.

In this study, the effects of stabilization methods (dry convective oven heating at 90 and 160 °C, microwave radiation at 180 and 360 W, and autoclave steaming) on both wheat germ and its oil were evaluated.

Steaming caused the most dramatic changes in lipoxygenase activity, free fatty acid content, DPPH radical scavenging activity, and mass fractions of tocopherols and tocotrienols. Lower peroxide values were measured in the oil samples treated with convectional heating (160 °C) and steaming at temperatures above 100 °C. However,
-anisidine values of samples treated at higher temperatures were considerably greater than those of samples stabilized at lower temperatures. Oven heating at 160 °C was also one of the most effective treatments, after steaming, for the inactivation of lipoxygenase. Steaming signifiide an advantage in oxidative stability compared to steaming and microwave applications. Steaming delayed oxidation in the germ, while further inhibiting lipoxygenase activity. Moreover, tocotrienols were more conservable. In industrial application, low-power microwave (180 instead of 360 W) and oven heating at lower temperature (90 instead of 160 °C) would be preferable.
In our study, spontaneous alcoholic fermentations were carried out to isolate non-
and
yeasts from grape must from different vine-growing regions in Slovenia. Additionally, the diversity of native
strains was evaluated during the process.

During spontaneous alcoholic fermentations the yeast population of non-
and
yeasts was sampled. We used eleven microsatellite markers to determine the genetic diversity of
strains. In addition, different ratios of the indigenous strains of
,
and
were tested for their possible use in alcoholic fermentation with inoculated yeasts by monitoring its course and measuring the concentration of aroma compounds in wine.

Sequencing of the internal transcribed spacer (ITS) regions of ribosomal DNA showed that of 64 isolates, 46 strains represent
and 18 strains belong to non-
yeasts. The identified non-
yeast species were
,
,
and
. The dendrogram grouped
strains into 14 groups. The number of
strains isolated from the musts was 10 (Posats, which allows the positive properties of the yeast strains to be expressed and good quality wines to be produced. Thus, the results are encouraging for winemakers to create different wine styles associated with a particular terroir using indigenous yeasts.
Wholewheat flour is a very good source of nutritional compounds and functional ingredients for human diet. However, it causes negative effect on bread quality. Different milling techniques can be used to obtain wholewheat flour, minimizing the negative effect of both bran and germ on bread quality. The aim of this work is to study the effect of particle size and shape of wholegrain flour on the interaction among the different components, water distribution, dough rheology and bread volume.

Wholewheat flour of three varieties (Klein Rayo, Fuste and INTA 815) was obtained in cyclonic, hammer and roller mills. The characteristics of wholewheat flour were explored, and the water distribution and rheological properties of dough were determined by thermogravimetric analysis and Mixolab test, respectively. Finally, microscale bread was prepared.

The amount of water-soluble pentosans, damaged starch and wet gluten was affected by the milling procedure. Regarding dough rheological properties, wholewheat flour obbread with better acceptance by consumers.
The effect of milling type and particle shape of the wholewheat flour had a greater effect than the wheat variety. Thus, the wholegrain milling process should be carefully selected taking to account the shape of the produced particle. This may open new opportunities for developing wholewheat bread with better acceptance by consumers.
The aim of this work is to evaluate utilization of sweet potato peel as a source of bioactive compounds. The effect of solvents (acetone and acetone/ethanol mixture) on the extraction efficiency of total carotenoids and phenolics from sweet potato tuber, flesh and peel, and antioxidant activity were investigated. Sweet potato peel extract stood out in terms of antioxidant activity and was chosen for encapsulation by spray and freeze-drying.

Encapsulation is an effective method to improve phytochemical stability by entrapping the core material with a coating agent. In this study, spray and freeze-drying techniques were applied for improving the stability of bioactive compounds (carotenoids and phenolics) using whey protein as a coating material. The main advantages of the applied techniques over the other encapsulation techniques are simplicity, continuity, effectiveness, availability and applicability.

Physicochemical characteristics revealed that spray drying resulted in the formation of lower size particles, better flow properties and encapsulation efficiency of carotenoids.
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