Notes

Phosphatidylserine receptors increase SARS-CoV-2 disease.
Conclusions 'You can't be what you can't see' women and people from many parts of the world cannot see themselves as chief editors, other editors or editorial board members of dental journals.Studying biomolecules in their native environment represents the ideal sample condition for structural biology investigations. Here we present a novel protocol which allows to delivery proteins into eukaryotic cells through a mild thermal stimulation. The data presented herein show the efficacy of this approach for delivering proteins in the intracellular environment of mammalian cells reaching a concentration range suitable for successfully applying biophysical methods, such as double electron electron resonance (DEER) measurements for characterising protein conformations.Epilepsy is a common neurological disorder characterized by recurrent unprovoked seizures. SCN1A encodes NaV1.1, a neuronal voltage-gated Na+ channel that is highly expressed throughout the central nervous system. NaV1.1 is localized within the axon initial segment where it plays a critical role in the initiation and propagation of action potentials and neuronal firing, predominantly in γ-amino-butyric-acid (GABA)ergic neurons of the hippocampus. The objective of this study was to characterize a de novo missense variant of uncertain significance in the SCN1A gene of a proband presented with febrile status epilepticus characterized by generalized tonic clonic movements associated with ictal emesis and an abnormal breathing pattern. Screening a gene panel revealed a heterozygous missense variant of uncertain significance in the SCN1A gene, designated c.4379A>G, p.(Tyr1460Cys). The NaV1.1 wild-type (WT) and mutant channel reproduced in vivo and were transfected in HEK 293 cells. Na+ currents were recorded using the whole-cell configuration of the patch-clamp technique. This NaV1.1 variant (Tyr1460Cys) failed to express functional Na+ currents when expressed in HEK293 cells, most probably due to a pore defect of the channel given that the cell surface expression of the channel was normal. Currents generated after co-transfection with functional WT channels exhibited biophysical properties comparable to those of WT channels, which was mainly due to the functional WT channels at the cell surface. The NaV1.1 variant failed to express functional Na+ currents, most probably due to pore impairment and exhibited a well-established loss of function mechanism. The present study highlights the added-value of functional testing for understanding the pathophysiology and potential treatment decisions for patients with undiagnosed developmental epileptic encephalopathy.A superconducting circuit with a qubit and a resonator coupled via a two-photon interaction is considered. When the resonator is initially in a superposition of coherent states, optical tomography and quantum coherence dynamics are examined in the context of intrinsic decoherence. find more The results reveal that optical tomography is a good quantifier of the quantum coherence produced by the qubit-resonator interaction. The effects of qubit-resonator detuning and intrinsic decoherence on the dynamics of optical tomography distributions for coherent and even coherent states are investigated. The dynamics of optical tomography distributions are highly dependent on detuning and intrinsic decoherence. Our numerical simulations reveal that there is a relation between the optical tomography and the generated quantum coherence. When the qubit-resonator detuning and intrinsic decoherence are augmented, the amplitude and intensity, as well as the structure of the optical tomography, change substantially.The family of elliptical gears with closed pitch curves includes elliptical gears and deformed elliptical gears. Elliptical gears are not only the most widely used non-circular gears but also the research hotspot in the field of non-uniform transmission. However, adjusting the shape of its pitch curve is difficult, which seriously restricts its popularization. This article proposes a piecewise deformed elliptical gear with a closed pitch curve, which realizes the unity of all kinds of elliptical gears in the mathematical model, makes the shape of the pitch curve and gear ratio easier to adjust, and expands the connotation and application scope of the family of elliptical gears. Moreover, the design approach of transmission with piecewise deformed elliptical gear was studied, and the inspection method of transmission performance was provided. The internal relationship between piecewise deformed elliptical gears and the family of elliptical gears was then analyzed. Finally, the method of computer-aided design (CAD) system developed was also provided. Additionally, a case was provided to verify the above theories. The designed conjugate pair can realize a correct meshing and adjust the gear ratio more flexibly, laying a theoretical foundation to expand the application field of non-uniform transmission.SARS-CoV-2 has spread throughout the world, including areas located at high or very high altitudes. There is a debate about the role of high altitude hypoxia on viral transmission, incidence, and COVID-19 related mortality. This is the first comparison of SARS-CoV-2 viral load across elevations ranging from 0 to 4300 m. To describe the SARS-CoV-2 viral load across samples coming from 62 cities located at low, moderate, high, and very high altitudes in Ecuador. An observational analysis of viral loads among nasopharyngeal swap samples coming from a cohort of 4929 patients with a RT-qPCR test positive for SARS-CoV-2. The relationship between high and low altitude only considering our sample of 4929 persons is equal in both cases and not significative (p-value 0.19). In the case of low altitude, adding the sex variable to the analysis, it was possible to find a significative difference between men and women (p-value  less then  0.05). Considering initially sex and then altitude, it was possible to find a significative difference between high and low altitude for men (p-value 0.05). There is not enough evidence to state that viral load is affected directly by altitude range but adding a new variable as sex in the analysis shows that the presence of new variables influences the relationship of altitude range and viral load. There is no evidence that viral loads (Ct and copies/ml) differ at low or high altitude. Using sex as a co-factor, we found that men have higher viral loads than women at low and moderate altitude locations, while living at high altitude, no differences were found. When Ct values were aggregated by low, moderate, and high viral load, we found no significant differences when sex was excluded from the analysis. We conclude that viral load is not directly affected by altitude, but COVID-19 incidence and mortality are rather affected by socio-demographic and idiosyncratic dynamics.SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.The development of successful treatment regimens for breast cancer requires strong pre-clinical data generated in physiologically relevant pre-clinical models. Here we report the development of the chick embryo chorioallantoic membrane (CAM) model to study tumor growth and angiogenesis using breast cancer cell lines. MDA-MB-231 and MCF7 tumor cell lines were engrafted onto the chick embryo CAM to study tumor growth and treatment response. Tumor growth was evaluated through bioluminescence imaging and a significant increase in tumor size and vascularization was found over a 9-day period. We then evaluated the impact of anti-angiogenic drugs, axitinib and bevacizumab, on tumor growth and angiogenesis. Drug treatment significantly reduced tumor vascularization and size. Overall, our findings demonstrate that the chick embryo CAM is a clinically relevant model to monitor therapeutic response in breast cancer and can be used as a platform for drug screening to evaluate not only gross changes in tumor burden but physiological processes such as angiogenesis.A data driven method-based robot joint fault diagnosis method using deep residual neural network (DRNN) is proposed, where Resnet-based fault diagnosis method is introduced. The proposed method mainly deals with kinds of fault types, such as gain error, offset error and malfunction for both sensors and actuators, respectively. First, a deep residual network fault diagnosis model is derived by stacking small convolution cores and increasing the core size. meanwhile, the gaussian white noise is injected into the fault data set to verify the noise immunity for the proposed deep residual network. Furthermore, a simulation is conducted, where different fault diagnosis methods including support vector machine (SVM), artificial neural network (ANN), convolutional neural network (CNN), long-term memory network (LTMN) and deep residual neural network (DRNN) are compared, and the simulation results show the accuracy of fault diagnosis for robot system using DRNN is higher, meanwhile, DRNN needs less model training time. Visualization analysis proved the feasibility and effectiveness of the proposed method for robot joint sensor and actuator fault diagnosis using DRNN method.The present study aimed to systematically examine whether laurinal, orange odor, and a specifically designed "perfume" influence sleep quality. During sleep, healthy participants (n = 139) were presented with odor or no odor through nose clips for fourteen consecutive nights (phase one). We collected physiological parameters together with subjective reports. Later on, longer lasting effects of this manipulation were examined for the following fourteen nights (phase two) without exposition to odors. Additionally, olfactory, cognitive and non-cognitive measures were conducted before phase one, between both phases and after phase two. One-way analyses of variance for repeated measures with nights and condition (1 vs 2) as the within-subject factor and odor condition (0, 1, 2 or 3) together with odor pleasantness rating as between-subject factor, was employed to analyse data. Overall, the present results demonstrated that the odor condition in comparison to control had no consistent effect on sleep in healthy participants which can be possibly explained by exposure to odors via nose clips. However, the analyses indicated that the individual pleasantness of odors enhanced the positive assessment of sleep quality. Altogether, the present results indicate that the subjective perception of an odor's hedonic value appears to be crucial for sleep quality, not the odors themselves.
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