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Network module analysis showed that one module dominated the network at each nutrient level (comprising 41%-65% of the nodes), indicating that AD community formed a core microbial guild. The most abundant phylotypes, Macellibacteroides and Butyricicoccaceae, were consistently negative with acetoclastic methanogens in the dominant modules. click here Their predominance at ≥15 g/L peptone can explain the hump-shaped responses of methanogenesis and methanogens. Collectively, methanogenesis and microbial network exhibited hump-shaped responses, although microbial community exhibited monotonic responses. Therefore, nutrient availability can determine the methanogenesis through regulating the relative fitness of methanogens within the community.Climate change has led to frequent drought events on the Mongolian Plateau; however, the pattern of preseason drought impacts on the start of the growing season (SOS) in grasslands is unclear. To determine how preseason drought controls the SOS in Mongolian Plateau grasslands, we quantitatively studied the sensitivity characteristics of the standardized precipitation evapotranspiration index-3 (SPEI-3) to the SOS and spatiotemporal differences based on meteorological and remote sensing datasets. The results show that 1) in the past 34 years, the regions with a trend for earlier SOS in Mongolian Plateau grasslands were mainly distributed in the typical steppe region, accounting for 30% of the total area, while 13% of the pixels showed a delayed trend, mainly occurring in desert steppes, and the remaining 57% of the pixels did not show a significant change (P 0.63; and 4) the sensitivity of the SOS to preseason drought significantly decreased from 1982 to 2015 in Mongolian Plateau grasslands. Our results can be used to improve the interpretation of the SOS response to drought in the land surface model.Tea plantation can cause strong soil degradation, e.g. acidification, basic nutrient decrease and microbial diversity loss, naturally by its root activity and secondary by practically tremendous synthetic N input. Organic amendments application is considered a practical way to mitigate the above adverse consequence. However, the trade-off between agronomic and environmental effects on the application of the organic amendments is still under debate. Herein, we conducted a long-term field experiment with four treatments, including control (without and fertiliser) (CK), chemical fertiliser treatment (CF), chicken manure treatment (CM) and chicken manure combined with biochar treatment (CMB) to investigate the effects of organic amendments application on soil quality, heavy metal contamination and tea production in a tea plantation. Totally 16 plots were arranged randomly with a completely randomised design. The results showed that CM and CMB treatments improved soil nutrient, mitigated soil acidification and amea quality.The typical tire manufacturing additive 6PPD, its metabolites 6PPDQ and 4-Hydroxy should be monitored because of their ubiquitous presence in the environment and the high toxicity of 6PPDQ to coho salmon. The toxic effect of 6PPD and its metabolites have been revealed superficially, especially on behavioral characteristics. However, the behavioral indicators explored so far are relatively simple and the toxic causes are poorly understood. With this in mind, our work investigated the toxic effects of 6PPD, 6PPDQ and 4-Hydroxy on adult zebrafish penetratingly through machine vision, and the meandering, body angle, top time and 3D trajectory are used for the first time to show the abnormal behaviors induced by 6PPD and its metabolites. Moreover, neurotransmitter changes in the zebrafish brain were measured to explore the causes of abnormal behavior. The results showed that high-dose treatment of 6PPD reduced the velocity by 42.4% and decreased the time at the top of the tank by 91.0%, suggesting significant activity inhibition and anxiety. In addition, γ-aminobutyric acid and acetylcholine were significantly impacted by 6PPD, while dopamine exhibited a slight variation, which can explain the bradykinesia, unbalance and anxiety of zebrafish and presented similar symptoms as Huntingdon's disease. Our study explored new abnormal behaviors of zebrafish induced by 6PPD and its metabolites in detail, and the toxic causes were revealed for the first time by studying the changes of neurotransmitters, thus providing an important reference for further studies of the biological toxicity of 6PPD and its metabolites.Forest succession is an important process regulating the carbon and nitrogen budgets in forest ecosystems. However, little is known about how and extent by which vegetation succession predictably affects soil CO2, CH4, and N2O fluxes, especially in boreal forest. Here, a field study was conducted along a secondary forest succession trajectory from Betula platyphylla forest (early stage), then Betula platyphylla-Larix gmelinii forest (intermediate stage), to Larix gmelinii forest (late stage) to explore the effects of forest succession on soil greenhouse gas fluxes and related soil environmental factors in Northeast China. The results showed significant differences in soil greenhouse gas fluxes during the forest succession. During the study period, the average soil CO2 flux was greatest at mid-successional stage (444.72 mg m-2 h-1), followed by the late (341.81 mg m-2 h-1) and the early-successional (347.12 mg m-2 h-1) stages. The average soil CH4 flux increased significantly during succession, ranging from -0onment/chemical properties affecting soil CO2 and N2O fluxes and soil CH4 fluxes, respectively, in the secondary forest succession process.Implementing an inter-regional synergistic control policy for fine particulate matter (PM2.5) and ground-level ozone (O3) could improve regional air quality. However, little is known about the effectiveness and accuracy of synergistic control region delineation. This study aimed to construct a network model and apply it to a case study of regional delineation in China at different scales to quantify the interactions between regions. Firstly, the Cumulative Risk Index (CRI) was proposed and quantified from a health risk perspective based on the daily mean PM2.5 and daily maximum 8-h average O3 concentrations from 2015 to 2020 in China. Then, the complex network topology parameters were introduced to determine the optimal threshold for different network constructions, and the Girvan-Newman (GN) algorithm was used to divide the network into independent regions. Results showed that the correlation between cities is more robust than that between provinces. There are four-seven major provincial-scale regions with strong synchronicity in CRI, suggesting that PM2.5 and O3 synergistic control policies shall be implemented jointly within these demarcated regions. Moreover, urban-scale CRI network analysis indicated that the existing key control areas (2 + 26 cities) need to be expanded to 40-50 cities and refined into seven independent urban regions. Meanwhile, the Fen-Wei Plain can be focused on six cities Xi'an, Baoji, Xianyang, Weinan, Yuncheng, and Tongchuan. This study could improve our understanding of the synergistic control regions for PM2.5 and O3 pollution, and the results could be used to develop joint control policies for both pollutants.Coronavirus pandemic started in March 2020 and since then has caused millions of deaths worldwide. Wastewater-based epidemiology (WBE) can be used as an epidemiological surveillance tool to track SARS-CoV-2 dissemination and provide warning of COVID-19 outbreaks. Considering that there are public places that could be potential hotspots of infected people that may reflect the local epidemiological situation, the presence of SARS-CoV-2 RNA was analyzed by RT-qPCR for approximately 16 months in sewage samples from five public places located in the metropolitan area of Belo Horizonte, MG, Brazil the sewage treatment plant of Confins International Airport (AIR), the main interstate bus terminal (BUS), an upscale shopping centre (SHC1), a popular shopping centre (SHC2) and a university institute (UNI). The results were compared to those of the influent sewage of the two main sewage treatment plants of Belo Horizonte (STP1 and STP2). Viral monitoring in the STPs proved to be an useful regional surveillance tool, reflecting the trends of COVID-19 cases. However, the viral concentrations in the samples from the selected public places were generally much lower than those of the municipal STPs, which may be due to the behaviour of the non-infected or asymptomatic people, who are likely to visit these places relatively more than the symptomatic infected ones. Among these places, the AIR samples presented the highest viral concentrations and concentration peaks were observed previously to local outbreaks. Therefore, airport sewage monitoring can provide an indication of the regional epidemiological situation. For the other places, particularly the UNI, the results suggested a greater potential to detect the infection and trace cases especially among employees and regular attendees. Taken together, the results indicate that for a regular and permanent sentinel sewage surveillance the sewage from STPs, AIR and UNI could be monitored.Climate change is imposing drier atmospheric and edaphic conditions on temperate forests. Here, we investigated how deep soil (down to 300 cm) water extraction contributed to the provision of water in the Fontainebleau-Barbeau temperate oak forest over two years, including the 2018 record drought. Deep water provision was key to sustain canopy transpiration during drought, with layers below 150 cm contributing up to 60% of the transpired water in August 2018, despite their very low density of fine roots. We further showed that soil databases used to parameterize ecosystem models largely underestimated the amount of water extractable from the soil by trees, due to a considerable underestimation of the tree rooting depth. The consensus database established for France gave an estimate of 207 mm for the soil water holding capacity (SWHC) at Fontainebleau-Barbeau, when our estimate based on the analysis of soil water content measurements was 1.9 times as high, reaching 390 ± 17 mm. Running the CASTANEA forest model with the database-derived SWHC yielded a 185 gC m-2 y-1 average underestimation of annual gross primary productivity under current climate, reaching up to 687 ± 117 gC m-2 y-1 under climate change scenario RCP8.5. It is likely that the strong underestimation of SWHC that we show at our site is not a special case, and concerns a large number of forest sites. Thus, we argue for a generalisation of deep soil water content measurements in forests, in order to improve the estimation of SWHC and the simulation of the forest carbon cycle in the current context of climate change.Surface water is threatened by trace metal pollution due to increasing anthropogenic activities. Therefore, an appropriate source identification was essential to reduce the ecological risk posed by the given pollutants. In this study, shallow and deep learning approaches trained by a registered environmental dataset of discharge sources were employed to classify the potential emission sources of trace metals in the Elbe River, Germany. The results showed that the overall concentration rank of the given metals was Zn (226.5 ± 526.5 μg·L-1) > Ni (5.6 ± 4.7 μg·L-1) > Cu (5.3 ± 5.8 μg·L-1) > As (3.3 ± 3.7 μg·L-1) > Pb (2.9 ± 5.2 μg·L-1) > Cr (1.8 ± 2.5 μg·L-1) > Cd (1.3 ± 3.1 μg·L-1) in seven tributaries and the mainstream of the Elbe River, among which the tributary Triebisch had the highest risk quotient over 86. Random Forest outperformed other algorithms with the highest Kappa median values of 0.59 and the lowest Hamming-loss values of 0.22 in extraction of the majority voted class. Then, the source apportionment conducted by random forest suggested that wastewater disposal and metal industrial emissions were the source contributors in the tributary Triebisch (probabilities 0.
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