Notes

Conformational Claims and Fluctuations in Endothelial Nitric Oxide Synthase beneath Calmodulin Legislation.
48pg/ml. In the group with postpartum blues, the range of postpartum mothers appeared to be at levels of 58.54. CONCLUSION Levels of hormone estradiol are lower in mothers who have postpartum blues. It is needed individual attention to postpartum mothers so that those detected with very low estradiol levels can get treatment and prevention as soon as possible. check details OBJECTIVE This study aimed to compare CRP between mother with PROM and mother with prolonged labor. METHODS It was a Cross-Sectional Study and sampling technique used was consecutive sampling, sample size was 50 mothers, consist of 25 PROM mothers and 25 prolonged labor mothers. Inclusion criteria were pregnant women over 37 weeks with PROM or prolonged labor, not suffering from HIV/AIDS, Hepatitis B, preeclampsia, eclampsia, kidney and diabetes mellitus. Blood was collected in both groups through vein. The blood was centrifuged to obtain serum and CRP was examined by the ELISA method in the laboratory of the Hasanuddin University Makassar. Data analyzed using Chi-square and independent T-test. RESULTS The mean CRP level of maternal with PROM was 11.00mg/l while the mean CRP protein level in prolonged labor was 16.00mg/l. Maternal CRP levels with prolonged labor are higher at 5.00mg/l compared to CRP PROM levels and it is statistically significant (p=0.002, p less then 0.05). CONCLUSION CRP levels of prolonged labor is higher compared to CRP PROM levels. L.U.HYPOTHESIS Osseointegration can be enhanced by introducing bioactive polyelectrolyte-multilayer films on implant surfaces. To guarantee films to function successfully in use, keeping structural integrity during implanting is necessary, which requires films with strong adhesion and cohesion to resist the mechanical damage. Catechol is considered as the origin of amazing adhesion of mussels. We hypothesize that catechol functionalization of polyelectrolytes enables film construction on implants in a non-aggressive way, and helps films resist mechanical damages during implanting. EXPERIMENTS With lipopolysaccharide-amine nanopolymersomes (NPs), catechol-functionalized hyaluronic acid and NPs (cHA, cNPs) as a polycation, polyanion and primer, respectively, catechol-functionalized polyelectrolyte-multilayer films (cPEMs) were constructed on substrates via Layer-by-layer self-assembly. Effects of catechol functionalization on construction, surface properties, assembly mechanisms, structural integrity, mechanical properties and cytotoxicity of cPEMs were studied. FINDINGS Self-adhesive cPEMs can be constructed on substrates, which grow exponentially and are driven by coordination, covalent bonding, electrostatic interactions, hydrogen bonding, etc. cPEMs with suitable catechol concentrations can resist mechanical damage to keep structural integrity in simulated clinical implantation, show stronger adhesion and cohesion than non-catechol-functionalized films in nanoscratch and nanoindentation tests, and are non-cytotoxic to MSCs. With excellent drug-loading and cytosolic-delivery capacity of NPs, cPEM is promising in improving osseointegration of implants. Calcium phosphates (CaPs) in the form of blocks are typically not satisfied for administration to osteoporotic patients because of their rapid resorption rate in vivo. However, injectable CaP powders have not been investigated for their potential in osteoporotic hosts. Herein, CaPs in the form of nanoparticles was reported can inhibit RANKL-stimulated osteoclastic differentiation (OC) and bone resorption, as evidenced by suppressed TRAP-positive cells, disintegrated F-actin rings and downregulated expression of markers for OC. CaP powders also significantly inhibited nuclear factor-κB (NF-κB) and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) activation. Furthermore, injectable CaPs reversed bone loss in a mouse model induced by lipopolysaccharide (LPS) and promoted osteoblastic formation in the absent of pro-osteogenic agents. Therefore, injectable CaPs, especially biphasic calcium phosphate (BCP), could be developed as novel agents for the therapy of osteolysis-related diseases caused by inflammation. V.Electronic structure and bioactivity of calcium phosphate (CaP) coatings on Ti-based anodic nanotubes are investigated. Nanotubes on pure Ti and Ti-6Al-4V alloy, respectively, are used as substrates for CaP deposition. The CaP coatings are formed by first growing a seeding CaP layer using alternative immersion (AIM) treatment followed by crystallization in Dulbecco's phosphate-buffered saline (DPBS). CaP coatings formed on both Ti and Ti-6Al-4V substrates are found containing a variety of bioactive CaP species, such as hydroxyapatite (HA), amorphous CaP (ACP), octacalcium phosphate (OCP), and dicalcium phosphate dihydrate (DCPD). The compositions of the coatings during the nucleation and crystallization processes are tracked and analyzed using X-ray absorption near-edge structure (XANES). The variation of CaP species in the resulted coatings are found strongly dependent on the choice of metal substrates, which leads to different bioactivities. By comparing the proliferation and differentiation of osteoblast cells (MC3T3-E1) on the CaP coatings, correlations between CaP species and their bioactivities are established. Difficult healing of skin wounds is one of the serious complications of diabetes mellitus. Green tea polyphenols (TP) have been found to have good therapeutic effects on wounds healing. However, TP that is soluble in water and easily been oxidized requires a gel material that provides moisture retention, oxidation prevention, and sustained release of TP to achieve better wound healing effect. Therefore, in this work, novel tea polyphenol nanospheres (TPN) were synthesized and encapsulated in a PVA /alginate hydrogel (TPN@H). The prepared TPN@H was characterized and applicated in model diabetic rats for promoting wound healing and regulating immune response. Fourier-transform infrared spectroscopy (FT-IR), UV spectroscopy, scanning electron microscopy (SEM), atomic force microscope (AFM), confocal laser scanning microscopy (CLSM), dynamic light scattering (DLS) and differential scanning calorimetry (DSC) were used for characterization. Animal experiments and molecular mechanism research proved that TPN@H could promote wound healing of diabetic rats by regulating PI3K/AKT signaling pathway. Bacterial infections of burn wounds are a significant problem that usually slows or stops the process of burn wounds healing. The use of topical antibiotics based on a novel drug delivery system could overcome the limitations of burn wound healing. In this work, the development of new wound dressings based on nanocomposite film of polyvinyl alcohol (PVA) and halloysite nanotubes (HNT) for the delivery of minocycline was investigated. link2 These elastomeric nanocomposites were prepared based on HNT surface modification by APTES and then PVA coating by LbL strategy. The resulting nanocomposites were characterized by FT-IR, XRD, zeta potential, Tg analysis, FESEM, and antibacterial studies. link3 The biodegradability and water uptake of the film were evaluated, the results of which revealed the absorption of scarring and non-degradation of the nanocomposite during treatment. Because minocycline decomposes by light, increasing photostability was another goal that was achieved. The release profile of the drug from the nanocomposite was studied, and it was found to be consistent with the Korsmeyer-Peppas model. In-vitro studies showed the antibacterial effect of nanocomposite on exposure to Gram-positive and Gram-negative bacteria. Due to the properties of the resulting nanocomposite film, it can be considered as a promising candidate for wound healing. In-vivo studies, cell culture, neuroprotective and anti-inflammatory effects may be investigated to develop this wound dressing in the future. Fentanyl is a pain reliever stronger and deadlier than heroin. This lethal drug has killed many people in different countries recently. Due to the importance of the diagnosis of this drug, a fentanyl electrochemical sensor is developed based on a glassy carbon electrode (GCE) modified with the carbon nanoonions (CNOs) in this study. Accordingly, the electrochemical studies indicated the sensor is capable of the voltammetric determination of traces of fentanyl at a working potential of 0.85 (vs. Ag/AgCl). To obtain the great efficiency of the sensor some experimental factors such as time, the potential of accumulation and pH value of the electrolyte were optimized. The results illustrated a reduction and two oxidation peaks for fentanyl in phosphate buffer (PB) with pH = 7.0 under a probable mechanism of electrochemical-chemical-electrochemical (ECE). The differential pulse voltammetry (DPV) currents related to the fentanyl detection were linear with an increase of fentanyl concentrations in a linear range between 1 μM to 60 μM with a detection limit (LOD) of 300 nM. Furthermore, the values of the diffusion coefficient (D), transfer coefficient (α) and catalytic constant rate (kcat) were calculated to be 2.76 × 10-6 cm2 s-1, 0.54 and 1.76 × 104 M-1 s-1, respectively. These satisfactory results may be attributed to utilizing the CNOs in the electrode modification process due to some of its admirable characterizations of this nanostructure including high surface area, excellent electrical conductivity and good electrocatalytic activity. Consequently, these finding points the achieving a simple sensing system to measure of the fentanyl as an important drug from the judicial perspective might be a dream coming true soon. Titanium and its alloys are the most widely used implants in clinical practice. However, their bioactivity is unsatisfactory, and the effect of osteogenesis on the bonding interface between the implant and bone needs to be further improved. In this study, a coating consisting of microporous titanium doped with silicon (Si-TiO2) was successfully created by microarc oxidation (MAO), and Si was evenly distributed on the surface of the coating. The surface morphology, roughness, and phase composition of the Si-TiO2 microporous coating were similar to those of the Si-free doped MAO coatings. The Si-TiO2 microporous coating can promote osteoblast adhesion, spreading, proliferation and differentiation. More importantly, the integrin β1-FAK signaling pathway may be involved in the regulatory effect of the coating on osteoblasts. Further studies in vivo indicated that the Si-TiO2 microporous coating could improve early stage osseointegration. In conclusion, the Si-TiO2 microporous coating is a feasible way to improve the osteogenic abilities of Ti implants to potentially promote clinical performance. Multifunctional nanoparticulate systems, especially those used in medicine, are currently of great interest. In this work, the in-vitro anticancer activity of As4S4/Fe3O4 composites dispersed in a water solution of Poloxamer 407 on breast MCF-7 and tongue SCC-25 cancer cells was verified. An increase in apoptotic cells as a consequence of higher caspase activities, a decrease in mitochondrial membrane potential and an accumulation of cells in the G2/M and subG0/G1 phases were detected after treatment with the As4S4/Fe3O4 nanosuspensions. The sterically stabilized nanosuspensions were characterized in relation to their particle size distribution, zeta potential and long-term stability properties. The interaction between the solid and liquid phases of the nanosuspensions was also studied using Fourier transform infrared spectroscopy.
Homepage: https://www.selleckchem.com/products/Gemcitabine-Hydrochloride(Gemzar).html