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The model illustrates promising performance overall, with particular skill in predicting above normal cyanobacteria biomass conditions which are of primary importance to lake and beach managers.Ulva prolifera green tide in the Yellow Sea of China is a typical cross-regional marine ecological disaster. We hypothesized that the complex interactions between U. prolifera and its associated bacterial communities possibly impact the formation and outbreak of green tide. To test this hypothesis, the U. prolifera-associated bacterial community changes in the entire migration area were investigated through field sampling and high-throughput sequencing. The results showed that (1) with the green tide migration, the richness and diversity increased for U. prolifera epiphytic bacterial communities, while they decreased for seawater bacterial communities in the phycosphere. (2) The richness, diversity, and community composition of U. prolifera-associated bacteria changed more dramatically in the 35.00°N sea area. (3) Potential interactions between bacteria and U. prolifera existed during the entire long-distance migration of green tide, and six bacterial functional groups (BFGs) were defined. Growth-regulating BFG I and antibacterial and stress-resistance BFG II were the dominant communities in the early stage of the green tide migration, which have the role of regulating algal growth and synergistic protection. Heterotrophic BFG III and algicidal BFG IV were the dominant communities in the late stage of the green tide migration, and they were able to compete with algae for nutrients and inhibit algal growth. Nutritive BFG V and algae-derived nutritional type BFG VI symbiotically lived with algal host. Our study highlights the spatial and temporal complexity of U. prolifera-associated bacterial communities and provides valuable insights into the potential contribution of U. prolifera-associated bacterial communities to green tide outbreaks.The effects of harmful algae on bivalve physiology are complex and involve both physiological and behavioural responses. Studying those responses is essential to better describe and predict their impact on shellfish aquaculture and health risk for humans. In this study we recorded for two months the physiological response of the blue mussel Mytilus edulis from Eastern Canada to a one-week exposure to a paralytic shellfish poisoning producing dinoflagellate strain of Alexandrium catenella, isolated from the St Lawrence estuary, Canada. Mussels in a 'control' treatment were fed continuously with a non-toxic diet, while mussels in a 'starvation' treatment were fed the same non-toxic diet the first week and subsequently starved for seven weeks. Mussels in a 'toxic' treatment received A. catenella for one week before being starved until the end of the experiment. Over a two-month experiment we monitored shell and tissue growth, filtration capacity, respiration rate, byssal attachment strength, valve opening behavixposed to the toxic dinoflagellate was also lower than in the control group. These results represent advancements in our understanding of the impacts of harmful algae on bivalves and contribute to the development of mitigation measures necessary to both the safety of consumers and the sustainability of aquaculture operations.Marine microalgae produce a variety of specialised metabolites that have toxic effects on humans, farmed fish, and marine wildlife. Alarmingly, many of these compounds bioaccumulate in the tissues of shellfish and higher trophic organisms, including species consumed by humans. Molecular methods are emerging as a potential alternative and complement to the conventional microscopic diagnosis of toxic or otherwise harmful microalgal species. Quantitative PCR (qPCR) in particular, has gained popularity over the past decade as a sensitive, rapid, and cost-effective method for monitoring harmful microalgae. Assays targeting taxonomic marker genes provide the opportunity to identify and quantify (or semi-quantify) microalgal species and importantly to pre-empt bloom events. Moreover, the discovery of paralytic shellfish toxin biosynthesis genes in dinoflagellates has enabled researchers to directly monitor toxigenic species in coastal waters and fisheries. This review summarises the recent developments in qPCR detection methods for harmful microalgae, with emphasis on emerging toxin gene monitoring technologies.Harmful algal blooms, including those caused by the toxic diatom Pseudo-nitzschia, can have significant impacts on human health, ecosystem functioning and ultimately food security. In the current study we characterized a bloom of species of Pseudo-nitzschia that occurred in a south-eastern Australian oyster-growing estuary in 2019. Using light microscopy, combined with molecular (ITS/5.8S and LSU D1-D3 rDNA regions) and toxicological evidence, we observed the bloom to consist of multiple species of Pseudo-nitzschia including P. cf. cuspidata, P. hasleana, P. fraudulenta and P. multiseries, with P. cf. cuspidata being the only species that produced domoic acid (3.1 pg DA per cell). As several species of Pseudo-nitzschia co-occurred, only one of which produced DA, we developed a rapid, sensitive and efficient quantitative real-time polymerase chain reaction (qPCR) assay to detect only species belonging to the P. pseudodelicatissima complex Clade I, to which P. cf. cuspidata belongs, and this indicated that P. cy and management outcomes.Monitoring of cyanobacterial bloom biomass in large lakes at high resolution is made possible by remote sensing. However, monitoring cyanobacterial toxins is only feasible with grab samples, which, with only sporadic sampling, results in uncertainties in the spatial distribution of toxins. To address this issue, we conducted two intensive "HABs Grabs" of microcystin (MC)-producing Microcystis blooms in the western basin of Lake Erie. These were one-day sampling events during August of 2018 and 2019 in which 100 and 172 grab samples were collected, respectively, within a six-hour window covering up to 2,270 km2 and analyzed using consistent methods to estimate the total mass of MC. The samples were analyzed for 57 parameters, including toxins, nutrients, chlorophyll, and genomics. There were an estimated 11,513 kg and 30,691 kg of MCs in the western basin during the 2018 and 2019 HABs Grabs, respectively. learn more The bloom boundary poses substantial issues for spatial assessments because MC concentration varied by nearly two orders of magnitude over very short distances. The MC to chlorophyll ratio (MCchl) varied by a factor up to 5.3 throughout the basin, which creates challenges for using MCchl to predict MC concentrations. Many of the biomass metrics strongly correlated (r > 0.70) with each other except chlorophyll fluorescence and phycocyanin concentration. While MC and chlorophyll correlated well with total phosphorus and nitrogen concentrations, MCchl correlated with dissolved inorganic nitrogen. More frequent MC data collection can overcome these issues, and models need to account for the MCchl spatial heterogeneity when forecasting MCs.Cyanobacterial harmful algal blooms (HABs) are increasing in frequency and magnitude worldwide. A number of parameters are thought to underlie HABs, including the ratio at which two key elements, nitrogen (N) and phosphorus (P) are supplied, although a predictive understanding eludes us. While the physiological importance of iron (Fe) in electron transport and N-fixation is well known, relatively little is known about its impacts on the growth of freshwater cyanobacteria. Moreover, there is growing appreciation for correlated changes in the quotas of multiple elements encompassing an organism (i.e. the ionome) when the supply of one element changes, indicating that growth differences arise from complex biochemical adjustments rather than limitation of a key anabolic process by a single element. In this study, the effects of supply NP and Fe on the growth and ionome of Dolichospermum, a nitrogen-fixing cyanobacterium found in freshwater ecosystems, were examined. Changes in both supply NP and Fe had significant effects on yield. Consistent with prior observations, cyanobacterial growth was higher at NP = 20, compared to NP = 5, and quotas of all elements decreased with growth. Yield was negatively related with the degree of imbalance between dissolved supply and intracellular concentrations of not only N and Fe, but also multiple other elements. Changes in Fe supply had a significant effect on yield in N-limited conditions (NP = 5). Again, ionome-wide imbalances decreased yield. Together, these results indicate that attention to multiple elements encompassing the ionome of a HAB-forming taxon, and the supplies of such elements may help improve the ability to forecast blooms. Such elemental interactions may be critical as limnologists begin to appreciate the staggering variation in the supplies of such elements among lakes, and anthropogenic activities continue to alter global biogeochemical cycles.INTRODUCTION An intrauterine contraceptive device (IUCD) is a common contraception method used for family planning. IUCD erosion into adjacent organs is a rare but serious complication of IUCD use. CASE PRESENTATION A 41-year-old female presented to us with a leaking left ectopic pregnancy. Emergency laparotomy and left salpingectomy were performed. A copper ICUD was found intraperitoneally and part of it had completely eroded into the sigmoid colon. Sigmoid colotomy was performed and the IUCD was removed successfully. Further history revealed that the patient had her IUCD inserted 12 years previously but was forgotten. The patient was discharged well after 4 days of admission. DISCUSSION Erosion of an IUCD into the colon is uncommon and may be asymptomatic or present with bowel perforation and obstruction. There should be a high index of suspicion for pregnancy occurring among women post-IUCD insertion. A misplaced IUCD can cause chronic inflammation of the fallopian tube, which may alter tubal functionality and increase the risk of ectopic pregnancy. Family planning is commonly done in primary health care. Primary care education and counselling are essential to improve awareness of fertile women to prevent similar complications. Periodic examination of IUCD string either by users or primary health-care practitioners is crucial. Ultrasound can be advocated if there are difficulties with the insertion. An abdominal radiograph is useful and should be performed in the case of missing IUCDs.INTRODUCTION Establishing the nurse practitioner (NP) workforce in New Zealand is a viable solution to health and workforce challenges in primary health care. General practices have been slow to implement NP services. Managers of general practices are central to the employment and development of NP roles. AIM To explore the perspectives of managers on employing NPs in general practice. METHODS An electronic survey was used to collect demographic and numerical data, which were analysed descriptively and analytically using SPSS (version 26). Written answers to open-ended questions were analysed qualitatively. RESULTS In total, 143 managers participated in the survey (response rate 39.7%); 54 (37.8%) worked in practices employing at least one NP. Of respondents, 88.9% (n = 127) agreed or strongly agreed that NPs could enhance continuity of care (89/143, 62.2%), improved access to services and medications (89/143, 62.2%) and filled a gap that added value to health care (97/143, 67.8%). Practices employing NPs had statistically significant higher levels of agreement about the advantages of NPs than practices not employing NPs.
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