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A protocol for constructing a standard drag cloth, establishing linear transects, and drag sampling is presented, along with a downloadable datasheet that can be modified to suit the needs of different projects. Efforts to align tick surveillance according to these standard best practices will help generate robust data on tick population biology.The two most economically important poultry ectoparasites are the northern fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), and the poultry red mite, Dermanyssus gallinae (De Geer). Both mites are obligate blood feeders but differ in where they reside. Sampling methods thus focus on-host, especially the vent feathers, for northern fowl mite and off-host, especially cracks and crevices near the nighttime roosting areas, for poultry red mite. Much remains unknown, however, about the basic biology and ecology of both mites. Here we discuss mite detection, quantification, and decision making and provide thoughts on future directions for research.Monitoring house fly (Diptera Muscidae) activity on animal facilities is a necessary component of an integrated pest management (IPM) program to reduce the negative impacts of these flies. This article describes monitoring methods appropriate for use on animal facilities with discussion of monitoring device use and placement. Action thresholds are presented where these have been suggested by researchers. Sampling precision is an important aspect of a monitoring program, and the number of monitoring devices needed to detect a doubling of fly activity is presented for monitoring methods where this information is available. It should be noted that both action thresholds and numbers of monitoring devices will be different for every animal facility. Suggested action thresholds and numbers of monitoring devices are presented only to provide guidance when initiating a fly monitoring program. Facility managers can adjust these values based upon the fly activity data recorded at their facility. Spot cards are generally recommended as an easy-to-use method for monitoring fly activity for most animal facilities. Fly ribbons or similar sticky devices are recommended where several pest fly species may be abundant and identifying the activity of each species is important, but a sampling period of less then 7 d may be needed in dusty conditions or when fly density is high. Fly ribbons are not recommended for outdoor use. Insecticide-baited traps may be used in outdoor locations where environmental conditions limit the use of spot cards, fly ribbons, and sticky traps.Deer keds (Diptera Hippoboscidae Lipoptena Nitzsch, 1818 and Neolipoptena Bequaert, 1942) are hematophagous ectoparasites of cervids that occasionally bite other mammals, including humans. In recent years, a number of arthropod-borne pathogens have been sequenced from deer keds. However, it is unclear if the pathogens are just present in host blood in the gut or if the pathogens are present in other organs (e.g., salivary glands) that would suggest that keds are competent vectors. Like other hippoboscoid flies, deer keds have extensive salivary glands that extend through the thorax and into the abdomen, so simply disarticulating and sequencing the thorax and abdomen separately does not circumvent the issues surrounding whole-body sequencing. SNX2112 Herein, we describe a technique for dissecting the terminal portion of the salivary glands from the abdomen in order to screen the thorax and salivary glands separately from the abdomen for arthropod-borne pathogens.Developing sampling programs for Culicoides can be challenging due to variation in ecology and behavior of the numerous species as well as their broad distributions and habitats. In this paper, we emphasize the need to clearly define research goals to select appropriate sampling methods. This includes not just the choice of sampling device, but also choice of attractant, site, number of traps per site, the duration and frequency of sampling, and the number of traps per unit area. Animal-baited trapping using enclosure traps and direct animal aspiration is more labor-intensive but yields information on species attracted to specific hosts as well as their biting rates. Sampling immatures is discussed with respect to choosing collection sites in semiaquatic mud, soil, and rich organic habitats. Sorting and extracting larvae using emergence traps, flotation, and Berlese funnels is also discussed.Arthropods pests are most frequently associated with both plants and vertebrate animals. Ticks, in particular the blacklegged ticks Ixodes scapularis Say and Ixodes pacificus Cooley & Kohls (Acari Ixodidae), are associated with wildlife hosts and are the primary vectors of Lyme disease, the most frequently reported vector-borne disease in the United States. Immature blacklegged ticks in the eastern United States frequently use small mammals from the genus Peromyscus as hosts. These mice are competent reservoirs for Borrelia burgdorferi, the causative agent of Lyme disease, as well as other tick-borne pathogens. To conduct surveillance on immature ticks and pathogen circulation in hosts, capture and handling of these small mammals is required. While protocols for rearing and pest surveillance on plants are common, there are very few protocols aimed at entomologists to conduct research on vertebrate-arthropod relationships. The goal of this manuscript is to provide a practical template for trapping Peromyscus spp. for vector and vector-borne pathogen surveillance and ecology for professionals that may not have a background in wildlife research. Important considerations are highlighted when targeting P. leucopus Rafinesque and P. maniculatus Wagner. Specifically, for tick and tick-borne disease-related projects, materials that may be required are suggested and references and other resources for researchers beginning a trapping study are provided.Live capture of white-tailed deer (Odocoileus virginianus) (Zimmermann, 1780) is often necessary for research, population control, disease monitoring, and parasite surveillance. We provide our deer trapping protocol used in a tick-host vector ecology research project and recommendations to improve efficiency of deer trapping programs using drop nets in suburban areas. We captured 125 deer across two trapping seasons. Generally, lower daily minimum temperatures were related to increased capture probability, along with the presence of snow. Our most successful trapping sites were less forested, contained more fragmentation, and greater proportion of human development (buildings, roads, recreational fields). To improve future suburban deer trapping success, trapping efforts should include areas dominated by recreational fields and should not emphasize remote, heavily forested, less fragmented parks. Concurrently, our study illustrated the heterogeneous nature of tick distributions, and we collected most ticks from one trapping site with moderate parameter values between the extremes of the most developed and least developed trapping sites.
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