Notes

LncRNA OIP5-AS1 knockdown or perhaps miR-223 overexpression may relieve LPS-induced ALI/ARDS by disturbing miR-223/NLRP3-mediated pyroptosis.
In total, 11 DEMs were successfully identified. An 11-miRNA risk signature and a prognostic nomogram were constructed based on the expression levels of these 11 DEMs and clinical information. The signature and nomogram were further validated by calculating the C-index, area under the curve (AUC) in receiver-operating characteristic curve analysis, and calibration curves, which revealed their promising performance. The results of the internal validation cohort shown the reliable predictive accuracy both of the miRNA-based signature and the prognostic nomogram. GO and KEGG analyses revealed that a mass of signal pathways participated in HNSCC proliferation and metastasis.

Overall, we constructed an 11-miRNA-based signature and a prognostic nomogram with excellent accuracy for predicting the OS of patients with HNSCC.
Overall, we constructed an 11-miRNA-based signature and a prognostic nomogram with excellent accuracy for predicting the OS of patients with HNSCC.
Copepods are fundamental components of pelagic food webs, but reports on how molecular responses link to reproductive success in natural populations are still scarce. We present a de novo transcriptome assembly and differential expression (DE) analysis in Temora stylifera females collected in the Gulf of Naples, Mediterranean Sea, where this copepod dominates the zooplankton community. High-Throughput RNA-Sequencing and DE analysis were performed from adult females collected on consecutive weeks (May 23rd and 30th 2017), because opposite naupliar survival rates were observed. We aimed at detecting key genes that may have influenced copepod reproductive potential in natural populations and whose expression was potentially affected by phytoplankton-derived oxylipins, lipoxygenase-derived products strongly impacting copepod naupliar survival.

On the two sampling dates, temperature, salinity, pH and oxygen remained stable, while variations in phytoplankton cell concentration, oxylipin concentration and oxylipf naupliar survival in natural populations of T. stylifera. Our results may help identify biomarker genes explaining variations in copepod reproductive responses at a molecular level.
Tall fescue (Festuca arundinacea Schreb.) is a major cool-season forage and turfgrass species. The low tiller density and size dramatically limits its turf performance and forage yield. MicroRNAs (miRNA)-genes modules play critical roles in tiller development in plants. In this study, a genome-wide small RNA profiling was carried out in two tall fescue genotypes contrasting for tillering production ('Ch-3', high tiller production rate and 'Ch-5', low tiller production rate) and two types of tissue samples at different tillering development stage (Pre-tillering, grass before tillering; Tillering, grass after tillering). 'Ch-3', 'Ch-5', Pre-tillering, and Tillering samples were analyzed using high-throughput RNA sequencing.

A total of 222 million high-quality clean reads were generated and 208 miRNAs were discovered, including 148 known miRNAs belonging to 70 families and 60 novel ones. Furthermore, 18 miRNAs were involved in tall fescue tiller development process. Among them, 14 miRNAs displayed increased lved in tiller development in cool-season turfgrass. Tillering related 18 miRNAs and their 212 target genes provide novel information for the understanding of the molecular mechanisms of miRNA-genes mediated tiller development in cool-season turfgrass.
This is the first genome-wide miRNA profiles analysis to identify regulators involved in tiller development in cool-season turfgrass. Tillering related 18 miRNAs and their 212 target genes provide novel information for the understanding of the molecular mechanisms of miRNA-genes mediated tiller development in cool-season turfgrass.
Texas Cristian University (TCU) psychosocial functioning and motivation scales were translated and validated for the persons who use drugs (PWUDs).

This study was carried out as a methodological research. The scales were translated forward and backward and then, the PWUDs in Kermanshah-based (west of Iran) substance abuse treatment centers in 2019 filled out the scales. Participants included 250 individuals who were selected by convenience sampling method. A demographics form and psychosocial functioning and motivation scales were used to collect data. For data analysis, content validity, face validity, test-retest, internal consistency, and construct validity were applied by using exploratory analysis methods in SPSS (v.20) and confirmatory factor analysis in LISREL software.

Exploratory Factor analysis (EFA) results were obtained by using main component analysis and Varimax rotation of three factors (psychological function, social function, treatment motivation). Extracted factors along with the eigen showed a total variance of the questionnaire. Moreover, the fitness indexes of the confirmatory factor analysis (CFA) of psychological function, social function, and treatment motivation were 0.059, 0.075 and 0.072, respectively. Therefore, the questionnaire fitness was at the acceptable level. The test-retest reliability coefficient was 0.875, and the Cronbach's alpha coefficient for all the components was higher than 0.71. + The results showed that psychosocial functioning, motivation scales have good psychometric properties, and they can be used in research and treatment fields.
Self-Defining Memories (SDMs) are a specific type of autobiographical memory, and that plays a key role in the construction of personal identity.

We investigated the characteristics of SDMs in elderly subjects. TAK-901 Aurora Kinase inhibitor The originality of the present study is to compare our elderly group to middle-aged subjects instead of young adults, as previously reported in the literature to understand the age-related modifications in SDMs.

We recruited 41 elderly subjects with normal cognitive functioning and 37 middle-aged adults. They were matched for education level and verbal knowledge.

Older participants recalled the same number of specific memories than middle-aged participants. SDMs were predominantly constituted of episodic characteristics, with specific details, in both groups. However, middle-aged subjects gave more integrative meaning of SDMs and more redemptive events than older participants. The two samples differed for three content dimensions (exploration/recreation, relationship contents, and not classifiable). As predicted, older participants reported memories that were more positive, on average, than the middle-aged participants' memories.

Our study added some contributions to the understanding of the consequences of aging on the self. Future research should explore the continuity of SDMs characteristics across the lifespan.
Our study added some contributions to the understanding of the consequences of aging on the self. Future research should explore the continuity of SDMs characteristics across the lifespan.Conventionally cardiac biomarkers are recognized as an essential tool to investigate the presence or progression of various cardiovascular diseases. However, in recent years data from several clinical trials have successfully sorted out the utility of cardiac biomarkers in diseases that are not primarily regarded as "cardiac diseases," especially neurological diseases. Results of freshly published trials have endorsed the use of cardiac biomarkers in various forms of stroke and dementia, including Alzheimer's disease. Alzheimer's disease is also one of the other CNS conditions where measuring cardiac biomarkers have found to be useful. Cardiac biomarkers can be helpful in two ways. Firstly, to assess the secondary involvement of the heart during the progression of the primary disease. Secondly, they can be useful in the diagnosis and prognosis of the primary condition itself. In this short review, we have collected encouraging results from recent studies that show the importance of the most widely recognized cardiac biomarkers in two of the most prominent neurological diseases of the current world, i.e., stroke and dementia.
Traumatic spinal cord injury (SCI) is a severe condition usually accompanied by an inflammatory process that gives rise to uncontrolled local apoptosis and a subsequent unfavorable prognosis. One reason for this unfavorable outcome could be the activation of the NLRP3 inflammasome.

MCC950 is a specific inhibitor of NLRP3 that further inhibits the formation of the NLRP3 inflammasome. The purpose of this study was to determine whether the NLRP3 inflammasome was associated with the severity of local apoptosis and whether MCC950 could prevent neuronal apoptosis following SCI.

In this study, primary cortical neurons were cultured in vitro. With or without pretreatment/posttreatment with MCC950, neurons were subjected to oxygen-glucose deprivation (OGD) for 2 h and then reperfusion for 20 h. Immunofluorescence was used to determine the expression of NLRP3, ASC and cleaved caspase-1 in neurons. In vivo, SCI model mice were established with a 5 g weight-drop method. MCC950 was intraperitoneally injected at 0, 2, preserving the survival of the remaining neurons, attenuating the severity of the damage, and promoting the recovery of motor function after SCI.
These results reveal that MCC950 exerts neuroprotective effects by reducing neuronal apoptosis, preserving the survival of the remaining neurons, attenuating the severity of the damage, and promoting the recovery of motor function after SCI.The emergence of severe acute respiratory syndrome (SARS) in Wuhan (china) named as corona virus disease 19(covid-19) caused by the novel corona virus SARS-CoV-2 has caused hundreds of thousands of mortalities across the world ,while the mortality rate is in millions, leading it to be declared as a global pandemic. Numerous research activities are undergoing to reveal the disease and etiological features of covid-19. In this review, some of the interesting aspects of covid-19 are discussed, that includes, the origin of the SARS-CoV-2, clinical manifestation, treatment and future aspects of the disease.
There is an urgent need to devise improved alternatives for the efficient delivery of drugs to develop improved therapeutic interventions against cancers. Nanotechnology based drug delivery vehicles are in-use with obvious issues of toxicity and bio-distribution. Therefore, green synthetic routes are being deployed to replace the conventional nanoparticle formulations for effective drug delivery aiming at developing interventional strategies against cancer.

A simple, viable and fast approach was used for the green synthesis of silver nanoparticles (AgNPs) using aqueous leaf-extract of Viburnum nervosum (VN) and to explore the anti-cancer potential of the crude extract of VN.

Silver NPs were synthesized by reacting silver nitrate (AgNO3) with leaf extract of VN. Various analytical techniques were used to characterize the AgNPs. Finally, the anti-cancer potential of VN was observed when delivered through AgNPs.

The surface plasmon spectra for AgNPs exhibited absorbance peak at 445 nm, and Fourier-Transform Infrared Spectroscopy investigation revealed presence of biomolecules acting as an effective reducing and capping agent for converting silver nitrate to AgNPs. Further, our results suggest the spherical size of synthesized AgNPs ranging from 12-17 nm. Moreover, in vitro studies conducted for VN extract with breast (MCF-7) and epidermal carcinoma (A431) cells showed biocompatibility.

Doxorubicin loaded AgNPs documented an increased bioavailability of drug compared to the free drug, suggesting the use of AgNPs as "novel drug delivery vectors".
Doxorubicin loaded AgNPs documented an increased bioavailability of drug compared to the free drug, suggesting the use of AgNPs as "novel drug delivery vectors".
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