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A great Evidence-Based Framework for Analyzing Pharmacogenomics Knowledge with regard to Individualized Medication.
Activation of the Wnt/β-catenin pathway is one of the hallmarks of colorectal cancer (CRC). Sirtuin 2 (SIRT2) protein has been shown to inhibit CRC proliferation. Previously, we reported that SIRT2 plays an important role in the maintenance of normal intestinal cell homeostasis. Here, we show that SIRT2 is a direct target gene of Wnt/β-catenin signaling in CRC cells. Inhibition or knockdown of Wnt/β-catenin increased SIRT2 promoter activity and mRNA and protein expression, whereas activation of Wnt/β-catenin decreased SIRT2 promoter activity and expression. β-Catenin was recruited to the promoter of SIRT2 and transcriptionally regulated SIRT2 expression. Wnt/β-catenin inhibition increased mitochondrial oxidative phosphorylation (OXPHOS) and CRC cell differentiation. Moreover, inhibition of OXPHOS attenuated the differentiation of CRC cells induced by Wnt/β-catenin inhibition. In contrast, inhibition or knockdown of SIRT2 decreased, while overexpression of SIRT2 increased, OXPHOS activity and differentiation in CRC cells. Consistently, inhibition or knockdown or SIRT2 attenuated the differentiation induced by Wnt/β-catenin inhibition. These results demonstrate that SIRT2 is a novel target gene of the Wnt/β-catenin signaling and contributes to the differentiation of CRC cells.
This study aims to analyze the incidence of Post-acute COVID-19 syndrome (PCS) and its components, and to evaluate the acute infection phase associated risk factors.

A prospective cohort study of adult patients who had recovered from COVID-19 (27th February to 29th April 2020) confirmed by PCR or subsequent seroconversion, with a systematic assessment 10-14 weeks after disease onset. PCS was defined as the persistence of at least one clinically relevant symptom, or abnormalities in spirometry or chest radiology. Outcome predictors were analyzed by multiple logistic regression (OR; 95%CI).

Two hundred seventy seven patients recovered from mild (34.3%) or severe (65.7%) forms of SARS-CoV-2 infection were evaluated 77 days (IQR 72-85) after disease onset. PCS was detected in 141 patients (50.9%; 95%CI 45.0-56.7%). Symptoms were mostly mild. Alterations in spirometry were noted in 25/269 (9.3%), while in radiographs in 51/277 (18.9%). No baseline clinical features behaved as independent predictors of PCS development.

A Post-acute COVID-19 syndrome was detected in a half of COVID19 survivors. Radiological and spirometric changes were mild and observed in less than 25% of patients. No baseline clinical features behaved as independent predictors of Post-acute COVID-19 syndrome development.
A Post-acute COVID-19 syndrome was detected in a half of COVID19 survivors. Radiological and spirometric changes were mild and observed in less than 25% of patients. No baseline clinical features behaved as independent predictors of Post-acute COVID-19 syndrome development.Consumption of energy drinks has been associated with adverse cardiovascular effects; however, little is known about the ingredients that may contribute to these effects. We therefore characterized the chemical profiles and in vitro effects of energy drinks and their ingredients on human induced pluripotent stem cell (iPSC)-derived cardiomyocytes, and identified the putative active ingredients using a multivariate prediction model. Energy drinks from 17 widely-available over-the-counter brands were evaluated in this study. The concentrations of six common ingredients (caffeine, taurine, riboflavin, pantothenic acid, adenine, and L-methionine) were quantified by coupling liquid chromatography with a triple quadrupole mass spectrometer for the acquisition of LC-MS/MS spectra. In addition, untargeted analyses for each beverage were performed with a platform combining LC, ion mobility spectrometry and mass spectrometry (LC-IMS-MS) measurements. Approximately 300 features were observed across samples in the untargeted studies, and of these ~100 were identified. In vitro effects of energy drinks and some of their ingredients were then tested in iPSC-derived cardiomyocytes. Data on the beat rate (positive and negative chronotropy), ion channel function (QT prolongation), and cytotoxicity were collected in a dilution series. We found that some of the energy drinks elicited adverse effects on the cardiomyocytes with the most common being an increase in the beat rate, while QT prolongation was also observed at the lowest concentrations. Finally, concentration addition modeling using quantitative data from the 6 common ingredients and multivariate prediction modeling was used to determine potential ingredients responsible for the adverse effects on the cardiomyocytes. These analyses suggested theophylline, adenine, and azelate as possibly contributing to the in vitro effects of energy drinks on QT prolongation in cardiomyocytes.Emotional eating, generally defined as (over)-eating in response to negative emotions, has been associated with poor physical and psychological outcomes. During a time of heightened negative affect, it is important to understand the impact of the COVID-19 pandemic and associated lockdown measures on eating behaviours, and further elucidate the ways in which emotional eating is related to emotion dysregulation and impaired abilities to identify emotions (i.e. alexithymia). The aims of this study were to explore perceived changes in eating behaviours in relation to self-reported negative affect during the pandemic and to examine direct and indirect effects of alexithymia on emotional eating. An online questionnaire measured these constructs in the general population of the United Kingdom (n = 136). Findings demonstrated that those who reported changes to their eating behaviours during the pandemic also reported greater levels of depression during the same time frame. Mediation analyses revealed that difficulties identifying and describing feelings both predicted emotional eating indirectly via emotion dysregulation. Findings contribute to the understanding of the mechanisms underpinning the relationship between alexithymia and emotional eating and describe changes to eating behaviours during COVID-19. We discuss how these findings should be applied, and recommendations for future research.At the onset of the coronavirus pandemic, sales of nonperishable food drastically increased in Germany. Reports of hoarding and panic buying flooded the media. To identify the drivers of the increased sales of these products, we carried out an online survey with n = 495 people living in Germany. We followed a concurrent triangulation design. For the quantitative analysis, we extended the theory of planned behavior to identify possible reasons for stockpiling nonperishable food. The results suggest that "attitude," "subjective norm," and the "fear of future unavailability" were main drivers of stockpiling behavior in our sample. Additionally, we analyzed answers to open questions capturing participants' own explanations of why they did or did not stockpile nonperishable food. By contrasting the results, we found that our qualitative results validate some of our quantitative findings but also deliver new insights. For instance, a key stated reason for stockpiling nonperishable food was to reduce shopping frequency.
The coronavirus disease 2019 (COVID-19) pandemic has caused unprecedented disruptions to the lives of families. This study aimed to investigate the impact of pandemic-associated stress on food parenting practices including interactions surrounding snacks, and child diet.

Parents (N=318) of 2-12-year old children completed a cross-sectional online survey assessing current COVID-19-specific stress, pre-COVID-19 stress, financial stress (e.g. food insecurity), food parenting practices, and child snack intake frequency. Structural Equation Modeling was used to model simultaneous paths of relationships and test direct and indirect effects.

Stress, including financial hardship, was higher compared with before the crisis. The majority of children had regular mealtimes and irregular snack times. Higher COVID-19-specific stress was associated with more non-nutritive use of food and snacks (e.g. this website emotional and instrumental feeding), but also more structure and positive interactions (e.g. eating with or engaging with child around mealtimes). Higher COVID-19-specific stress was also associated with greater child intake frequency of sweet and savory snacks, with some evidence for mediation by snack parenting practices.

Our findings indicate that stress associated with the COVID-19 pandemic may be linked to child snack intake with potential impacts on child obesity risk, and suggest several modifiable points of intervention within the family context.
Our findings indicate that stress associated with the COVID-19 pandemic may be linked to child snack intake with potential impacts on child obesity risk, and suggest several modifiable points of intervention within the family context.Previous research has shown that nudging can effectively support people's healthy food choices. Yet, to date knowledge about the psychological premises of nudging is limited, highlighting the need for closer scrutiny to determine how and when nudging is most effective. In the current study, we assessed whether the presumed effect of nudging on healthy food choice is enhanced under time pressure, a condition probing alleged system 1 reasoning. Food choice was studied in a realistic virtual reality supermarket where healthier alternatives were nudged by making them more salient. We additionally explored possible differences in decision-making experiences related to nudging or time pressure. The study took place at a science festival where visitors could decide to participate in a study. Participants (n = 99) had to purchase four products, each from a different product category that was provided on a shopping list. In the nudging condition, one healthier option within each product category was nudged by making it more salient. While a main effect of nudging was found, showing in increased healthy food choices, this effect was not further qualified by time pressure, suggesting that the effectiveness of nudging is not enhanced under system 1 conditions. Relatedly, people who were and who were not aware of the nudges showed similar effects of nudging on healthy food choice. Furthermore, no differences in decision-making experiences showed, suggesting that people have similar experiences regarding impulsive and reflective decision-making irrespective of whether they are being nudged or put under time pressure. All in all, our findings are in line with recent viewpoints on the premises of nudges, suggesting that alleged system 1 conditions are not a prerequisite for nudging to be effective.Water-content based electrical properties tomography (wEPT) can retrieve electrical properties (EPs) from water-content maps. B1+ field information is not involved in the traditional magnetic resonance electrical properties tomography approach. wEPT can be performed through conventional MR scanning, such as T1-weighted spin-echo imaging, which provides convenient access to multiple clinical applications. However, the inhomogeneous radiofrequency (RF) field induced by RF coils would cause inaccuracy in wEPT reconstructions during MR scanning. We conducted a detailed investigation to evaluate the effect of inhomogeneous RF field on wEPT reconstructions to guarantee that EP mapping is desired for clinical practice. Two important considerations are involved, namely, multiple typical coil configurations and various flip angles (FAs). We proposed a correction scheme with actual FA mapping to calibrate the RF inhomogeneity and finally validated it by using human imaging at 3 T. This study illustrates a detailed evaluation for wEPT under imperfect RF homogeneity and further provides a feasible correction procedure to mitigate it.
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