Notes

Fine-Tuning Cas9 Action with a Cognate Inhibitor AcrIIA4 to Improve Genome Editing within Streptomyces.
Classification accuracy was determined using analysis of variance. Input features were evaluated through a Jaccard index. From 68 vesico-urethral anastomoses, we analyzed 1,570 stitches broken down into 4,708 sub-stitches. For both classification tasks, ColumnSet best distinguished experts (n = 8) versus novices (n = 9) and ordinary experts (n = 5) versus super experts (n = 3) at an accuracy of 0.774 and 0.844, respectively. Feature ranking highlighted Endowrist articulation and needle handling/targeting as most important in classification. Surgeon performance measured by automated performance metrics on a granular sub-stitch level more accurately distinguishes expertise when compared with summary automated performance metrics over whole stitches.
The purpose of the study was to evaluate the association between microfibrillar collagen hemostat and anastomotic leakage after anterior resection.

Between March 2015 and December 2019, a total of 203 consecutive rectal cancer patients who underwent elective anterior resection were included. Patient parameters were analyzed. The relevant risk factors were identified by univariate and multivariate analysis. Propensity score matching was performed to reduce the selection bias.

In total, 26 (12.8%) of the 203 study patients developed clinical anastomotic leakage. The length of hospital stay was significantly prolonged by anastomotic leakage. In univariate analysis and multivariate analysis, male sex, low tumor location, and intraoperative application of microfibrillar collagen hemostat significantly increased the risk of anastomotic leakage. Furthermore, analysis after propensity score matching confirmed the independent role of microfibrillar collagen hemostat in anastomotic leakage. In addition, the mediatrolled trials are needed to confirm this association in the future.
Up to 50% of patients diagnosed with colorectal cancer develop metastases during the course of their disease. Surgical resection remains the only curative treatment option for colorectal liver metastases (CRLM), frequently in conjunction with neoadjuvant chemotherapy. This study sought to determine if the pathologic size of the largest CRLM impacted disease-free survival (DFS) and disease-specific survival (DSS) in the setting of neoadjuvant chemotherapy.

All patients diagnosed with CRLM who underwent neoadjuvant chemotherapy for liver resection at the Massachusetts General Hospital between 2004 and 2016 were reviewed. The median size of the largest liver lesion was used as the cutoff for grouped evaluation.

A total of 214 patients were included. Median follow-up was 100.0mo (interquartile range 68.9-133.8mo). The median size of the largest lesion was 21mm. Patients with lesions ≥21mm exhibited significantly worse median DFS (12.5mo versus 16.6mo; P=0.033) and median DSS (71.3mo versus 103.5mo; P=0.038). CRLM lesions ≥21mm were associated with poorer DFS on univariate analysis (hazard ratio (HR)=1.42, 95% confidence interval (CI) 1.03-1.95 P=0.033) and multivariable analysis (HR=1.58, 95% CI 1.07-2.35, P=0.023). CRLM lesions ≥21mm were also independently associated with poorer DSS after liver resection on univariate analysis (HR=1.51, 95% CI 1.02-2.24; P=0.037) and multivariable analysis (HR=1.98, 95% CI 1.27-3.07; P=0.002).

The size of the largest CRLM is an important prognostic factor for both DFS and DSS after neoadjuvant therapy and serves as a useful indicator of tumor biology.
The size of the largest CRLM is an important prognostic factor for both DFS and DSS after neoadjuvant therapy and serves as a useful indicator of tumor biology.
Pancreaticoduodenectomy with porto-mesenterico-splenic confluence resection can cause sinistral portal hypertension (SPH), which may lead to gastrointestinal bleeding. Nevertheless, it remains difficult to predict SPH development during surgery. The aim of this study is to assess the feasibility of measuring splenic vein (SV) pressure to predict SPH.

The patients who underwent pancreaticoduodenectomy with porto-mesenterico-splenic confluence resection between January 2016 and December 2017 were included in this study. SV pressure was measured before SV clamping (SVP1) and after SV clamping (SVP2). BL918 SPH was defined as varicose vein formation detected by follow-up computed tomography. Incidence of SPH was assessed in patients who had no SV drainage after surgery.

SV pressure was measured in 41 patients. Among them, 24 had no SV drainage (13 patients had occluded SV reconstruction, and 11 had SV ligation without an attempt at reconstruction) and were included for the analysis. SPH was observed in 16 of 24 patients (67%). The median ΔSVP (SPV2-SVP1) in patients with SPH was higher than that in patients without SPH (13.5mmHg versus 7.5mmHg, P=0.0237). Most patients with SVP2 >20mmHg (12/14 [86%]) or ΔSVP >10mmHg (10/11 [91%]) developed SPH.

For the patients with SV resection, high SV pressure after clamping (≥20mmHg) and a large SV pressure difference (≥10mmHg) before and after clamping are feasible indication criteria for SV reconstruction to prevent SPH.
For the patients with SV resection, high SV pressure after clamping (≥20 mmHg) and a large SV pressure difference (≥10 mmHg) before and after clamping are feasible indication criteria for SV reconstruction to prevent SPH.
Despite an increase in the number of practicing female physicians, gender disparities in academic medicine persist. For investigating gender gap in the transplantation field, this study examined the relationship between gender and authorship among medical and surgical transplant physicians.

In this observational study, all original clinical science articles published in the journals of Transplantation, American Journal of Transplantation, and Clinical Transplantation were reviewed from January 2008 to December 2017. Chi-square analysis was used to compare the proportions of female and male authors, and the Cochrane-Armitage test was used for comparisons over time.

A total of 2530 publications and 2988 individual authors met the inclusion criteria for the study. Male physicians published significantly more articles compared to female physicians as first (67.4% versus 30.4%) and senior authors (82.9% versus 16.2%), respectively. There were increases in the proportion of female first and senior authors between 2008 and 2017. The majority of authors with multiple publications were male (73.6%), specifically male medical physicians (44.3%). Male medical physicians were the most productive in publication amount and authorship positions.

While research activity among female physicians increased over time, gender disparity continues to exist among female and male physicians in the transplantation field. Academic activity is lower among females in publication amount and authorship positions. These trends emphasize the need to identify barriers to female physician academic productivity within the transplantation field.
While research activity among female physicians increased over time, gender disparity continues to exist among female and male physicians in the transplantation field. Academic activity is lower among females in publication amount and authorship positions. These trends emphasize the need to identify barriers to female physician academic productivity within the transplantation field.Herein, an induced self-enhanced electrochemiluminescence (ECL) sensor with superior ECL performances was simply fabricated by just dropping the ECL reagent (tris(4,4'-dicarboxylicacid-2,2'-bipyridyl) ruthenium (II) dichloride, Ru(dcbpy)3Cl2) and coreactant (nitrogen-doped carbon quantum dots, NCQDs) pair onto the surface of glassy carbon electrode. In this strategy, based on the carboxyl (-COOH) groups in Ru(dcbpy)32+ and oxygen, nitrogen-containing groups on NCQDs surface, an intermolecular hydrogen bonds-induced self-enhanced ECL composite was generated in the solid contact layer for the first time. Since Ru(dcbpy)32+ and NCQDs were co-existing in the same composite, the electron-transfer distance between them was shortened and the energy loss was decreased, thereby higher ECL efficiency was acquired. This working process greatly avoided the introduction of signal amplifier and simplified the experimental operation. On this basis, 17β-estradiol (E2) was selected as a target model to fabricate a self-enhanced ECL aptamer sensor for the investigation of its analytical performances. Resultantly, excellent detection properties of E2, including wider linear range of 1.0 × 10-14 - 1.0 × 10-6 mol L-1 and lower detection limit of 1.0 × 10-15 mol L-1 with superior selectivity, were successfully achieved. Finally, E2 spiked into milk powder was quantified to assess the practicability of this sensor. Prospectively, this strategy could be extensively applied for other analytes determination by adjusting the corresponding target aptamers.The growing application domain of energy storage devices (ESDs) is leading research to temperature tolerant supercapacitors. To realize reliable and safe devices, high modulus solid electrolytes are favored by most researchers. However, the inferior infiltrating ability of such electrolytes usually results in poor electrochemical performances of the ESDs. Herein, we adopted a hierarchical optimization strategy to address the aforementioned interfacial issues. Continuous ionic percolation throughout the hierarchical pores of the 3D electrode was formed by in-situ introducing an ionogel buffer layer. Benefiting from this, the rate of ions diffusing within electrodes was increased by 5 times. Furthermore, the kinetics of ions entering into nanopores was improved via introducing small size ions into ionic liquids (ILs) and adjusting the solvated structures. Both the capacity and rate performance of the electrochemical double layer capacitors (EDLCs) were improved. Additionally, the buffer layer exhibited sufficient thermostability to cooperate with poly(ether ether ketone) (PEEK)-based solid electrolyte. Consequently, the EDLCs exhibited excellent cycling stability (79% capacitance retention after 5000 cycles) at 120 °C and delivered a maximum energy density of 46.9 Wh kg-1 with a power density of 926.9 W kg-1. Our strategy is believed to be effective to cooperate with various solid electrolyte systems and offer a general design principle for durable and high performance EDLCs.Semi-transparent TiO2/graphene photoanodes are prepared at room temperature via an electrophoretic deposition method followed by compression and applied in dye-sensitized solar cells (DSSCs). Compression enhances the power conversion efficiency (PCE) of a DSSC, which constitutes up 18.4 times improvement compared to the uncompressed device. Incorporating graphene into the compressed film further improves the PCE by 28.8%. Simultaneously, compressing and graphene incorporating can greatly increase the film's transmittance at long wavelengths, benefiting to the use of DSSCs as front unit in tandem solar cells. Scanning electron microscopy, porosity measurements, electrochemical impedance spectroscopy and open circuit voltage decay are performed to investigate the mechanisms. It is demonstrated that compressing a film can reduce the porosity and improve the inter-particle connections, which accounts for the increased light transmittance and enhanced PCE. The incorporated graphene can provide extra charge carrier pathway due to its excellent charge transport properties, as well as protect TiO2 nanostructure by preventing film cracking upon pressing due to its good flexibility, thus increases PCE to 6.
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