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Introduction in the more uncommon cannabinoid Δ8 -Tetrahydrocannabinol within a doping trial.
748 - 0.925). When the cutoff was 4.746, the sensitivity and specificity were 86.9% and 91.5%, respectively. More importantly, peripheral blood miR-148 combined with ESR and hs-CRP demonstrated better diagnostic efficiency in active UC patients. Conclusions In summary, enhanced expression of miR-148 in peripheral blood of UC patients may be expected to be an index for evaluating the activity of UC disease.Background The objective of this study is to investigate the correlation of mean platelet volume (MPV), MPV/ platelet count, and monocyte to lymphocyte ratio (MLR) between cervical cancer patients and healthy people and to evaluate the value of those parameters in early diagnosis of cervical cancer. Methods The study population included 137 cervical cancer patients undergoing hysterectomy and 113 healthy controls. The clinical features (age, pathology type, tumor staging, and tumor size) were collected from the hospital information system. The hematology parameters (white blood cell, red blood cell, hemoglobin, platelet count, neutrophil, lymphocyte, monocyte, mean platelet volume, platelet distribution width) are obtained in the laboratory information system. Results We found that the monocyte count and MLR value are higher in the cervical cancer group. The MPV and MPV/platelet are lower in the cervical cancer group. The receiver operating characteristic (ROC) analysis shows that MPV+MLR can generate a moderate specificity with 71.68%, sensitivity with 65.69%, and AUC with 0.718 to distinguish cervical cancer and healthy people. Conclusions MPV/platelet and MLR may be helpful for the early diagnosis of cervical cancer. A larger clinical data analysis is necessary to evaluate the diagnostic value of hematologic parameters in cervical cancer.Background Anemia is one of the most common hematological problems in HIV infected patients in the world. The main aim of this study was to determine the magnitude of anemia and associated factors among HIV infected children on highly active antiretroviral therapy attending University of Gondar Comprehensive and Specialized Referral Hospital. Methods A retrospective study was conducted from 2013 to 2018 by reviewing medical records at University of Gondar Comprehensive and Specialized Referral Hospital ART clinic. Records of 238 HIV infected children on HAART were selected using a convenient sampling technique. Socio-demographic characteristics, clinical information, and hematological and immunological profiles of the study participants were collected from the patients record books. WHO cutoff value of hemoglobin was taken and adjusted to define anemia in higher altitude. Data was analyzed by using the SPSS version 20 statistical software, and odds ratios with 95% confidence intervals were used to quantify the strength of association between anemia and its potential predictors. Results The overall prevalence of anemia among HIV infected children in this study was 38.7%. CW069 Microtubule Associat inhibitor Of anemic children, 48.9% had mild, 39.1% moderate, and 11.9% severe anemia. This study showed that HIV infected children on Highly Active Antiretroviral Therapy who live in rural residence had a two-fold risk of being anemia than urban dwellers (AOR = 2.151, 95% CI, 1.123 - 4.122). There was no significant association with gender, WHO clinical stage, opportunistic infections, cotrimoxazole treatment, and CD4 count percentage. Conclusions Anemia is a common problem among the children taking highly active antiretroviral therapy. Therefore, health care workers need to routinely investigate and treat anemia, especially in rural dwellers. Furthermore, large scale and longitudinal studies are recommended to strengthen and explore the problem in depth.Background Applicability of thrombin generation tests to clinical routine has been sought for many years. The aim of this study was to compare thrombin generation measured in fresh platelet poor plasma (f-PPP) and frozen-thawed platelet poor plasma (ft-PPP) to prove the consistency of results. Methods In this prospective study, thrombin generation was measured in twenty-fold repetitions in 3.2% citrate PPP obtained from male healthy blood donors aged 19 - 39 years (n = 54 donations). The tests were performed with fresh PPP and repeated after storing the PPP at -60°C. In two subgroup analyses, the effect of higher and lower normal baseline platelet counts on the Calibrated Automated Thrombogram (CAT) assay and the influence of ABO blood groups on thrombin generation were analyzed. Results Referring to the parameters of thrombin generation most frequently used in studies, peak thrombin of f-PPP and ft-PPP agreed in about 50% of the samples. Endogenous thrombin potential (ETP) of f-PPP and ft-PPP agreed in nearly two-thirds of the samples. A slightly but significantly slower kinetic was found in the thrombin generation of ft-PPP compared with f-PPP. At least in f-PPP, ETP correlates with baseline platelet counts of the whole blood sample. Peak thrombin was significantly higher in non-O blood groups compared to O blood group. Conclusions A low level of agreement between the results of f-PPP and ft-PPP is shown. In terms of practicability of sample collection using semi-automated thrombin-generation assays ft-PPP should be preferred over f-PPP. We therefore recommend using ft-PPP in clinical studies.Background Pulmonary hypertension (PH) is a commonly observed complication of chronic obstructive pulmo-nary disease (COPD) which may lead to poor prognosis of the disease. The present study aims to explore the roles of circulating miR-210 for the early diagnosis of COPD included PH. Methods We included 80 patients with COPD including PH, 80 patients with COPD but without PH, and 80 healthy subjects as the study population. The plasma of each patient was collected, and the expressions of miR-210 in different groups were compared by RT-qPCR method. The diagnostic value of miR-210 was evaluated by ROC curve, and the correlation between the plasma level of miR-210 and systolic pulmonary artery pressure (SPAP) as well as pulmonary function index forced expiratory volume in the first second (FEV1) and forced vital capacity (FVC) were also analyzed. Results MiR-210 was significantly increased in plasma of COPD patients with PH compared with the COPD patients without PH. The results of ROC analysis showed that miR-210 is a sensitive biomarker to distinguish COPD + PH patients from the COPD patients. Moreover, the expression level of miR-210 in the plasma was positively correlated with the SPAP and negatively correlated with the FEV1 and FVC of the COPD + PH patients. Conclusions MiR-210 was up-regulated in plasma of patients with COPD included PH, and miR-210 may serve as an early diagnostic marker for the disease.Background Cryoglobulins and hyperviscosity syndrome (HS) sometimes occur in multiple myeloma (MM), which are considered clinical emergencies. In laboratory practice, aspiration failure in routine blood tests sometimes occurs when the sample is inadequate. Here, a case of cryoglobulinemia and HS associated with advanced multiple myeloma was reported, which unusually is initially confirmed by aspiration failure in a routine blood test with sufficient sample. Methods A case of a 48-year-old female whose diagnosis of cryoglobulinemia and hyperviscosity syndrome secondary to MM-IgA kappa was confirmed from routine blood test. Results The sufficient sample for routine blood test could not be analyzed in a hematology analyzer due to aspiration failure, which was found to be caused by high viscosity and poor liquidity. A peripheral blood smear showed numerous non-cellular clouds, erythrocyte rouleaux formation, and plasma cell infiltration. After a water bath, the non-cellular clouds evidently disappeared, and the routine blood test was successfully conducted. Centrifugation of the sample for biochemical test, which had previously failed, was also possible. The case was confirmed as complications of cryoglobulinemia and HS associated with advanced MM, and the non-cellular clouds were identified as cryoglobulins. Conclusions This case report provides an effective way for clinicians to deal with this kind of abnormal sample and limited but important laboratory evidence to establish early diagnosis of cryoglobulinemia and HS secondary to MM.Background The current study aims to investigate the expression and significance of lncRNA PANDAR in the serum of patients with type II diabetes mellitus (T2DM) and diabetic nephropathy (DN). Methods The expression of PANDAR in 77 T2DM patients, 60 DN patients, and 60 healthy controls was detected by RT-PCR. Pearson's correlation assay was carried out to analyze the correlation between serum lncRNA PANDAR and clinical indicators. Receiver operator characteristic (ROC) analysis was carried out to analyze the diagnostic value of PANDAR in T2DM and DN patients. Results The expression of PANDAR in T2DM and DN patients was significantly higher than that in the control group. Moreover, the expression of PANDAR in DN patients with massive proteinuria was significantly higher than that in DN patients with microalbuminuria. Further study showed that the expression of PANDAR was positively correlated with the level of proteinuria (r = 0.690, p less then 0.001), and negatively correlated with the glomerular filtration rate (r = -0.780, p less then 0.001). In T2DM and DN patients, the area under ROC curve (AUC) of PANDAR as serum marker was 0.861 (95% CI 0.786 - 0.935, p less then 0.001), between DN patients and T2DM patients, while the AUC of PANDAR as a marker in diabetic nephropathy was 0.914 (95% CI 0.828 - 0.980, p less then 0.001) between DN patients and healthy controls. Conclusions In summary, the high expression of PANDAR is related to the development of DN in T2DM patients, and it is expected to be a biomarker for predicting the prognosis of DN patients.Background Acute myocardial infarction (AMI) is myocardial necrosis caused by acute and persistent ischemia and hypoxia of coronary arteries. AMI is one of the most common diseases in European countries and over 1.5 million AMI patients die of it in the United States annually. A collection of studies proposed that certain micro-RNAs play crucial roles in the onset and development of AMI. Methods Ninety-four AMI patients and 83 non-AMI healthy controls were recruited from Zhongda Hospital, Southeast University between July 2015 and September 2017. Serum samples were collected at admission and the expression of miR-142 was detected using real-time quantitative polymerase chain reaction (RT-qPCR) assays. Results miR-142 expression was markedly elevated in serum samples of AMI patients compared with the 83 non-AMI healthy controls. miR-142 expression was positively correlated with creatine kinase-KB (CK-MB; r = 0.6731, p = 0.0021) and troponin (r = 0.7138, p = 0.0013). The area under the curve (AUC) of miR-142, CK-MB, and troponin for the diagnosis of AMI were 0.9185, 0.8172, and 0.8717, respectively. Overall survival analysis implied that high miR-142 expression may predict poor survival (log-rank test, p = 0.0146). Conclusions miR-142 may be a diagnostic and prognostic indicator for AMI, and therefore, it may contribute to AMI clinicopathologic prediction.
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