Notes

Manufacturing of a live cell-containing multilayered plastic hydrogel membrane with micrometer-scale thickness to gauge pharmaceutical exercise.
In addition to being necessary for a complete account of behavior, such data and theories might contribute to improved interventions for problems of human concern.Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19), which may result in acute respiratory distress syndrome (ARDS), multiorgan failure, and death. The alveolar epithelium is a major target of the virus, but representative models to study virus host interactions in more detail are currently lacking. Here, we describe a human 2D air-liquid interface culture system which was characterized by confocal and electron microscopy and single-cell mRNA expression analysis. In this model, alveolar cells, but also basal cells and rare neuroendocrine cells, are grown from 3D self-renewing fetal lung bud tip organoids. These cultures were readily infected by SARS-CoV-2 with mainly surfactant protein C-positive alveolar type II-like cells being targeted. Consequently, significant viral titers were detected and mRNA expression analysis revealed induction of type I/III interferon response program. Treatment of these cultures with a low dose of interferon lambda 1 reduced viral replication. Hence, these cultures represent an experimental model for SARS-CoV-2 infection and can be applied for drug screens.
Dermatomyositis (DM) and polymyositis (PM) are two rare autoimmune disorders occasionally described with dysthyroidism; however, no solid evidence still proves such an association.

To evaluate the prevalence of dysthyroidism among DM/PM patients.

A nation-wide case-control study was conducted.

From the Clalit Health Services health records database, we extracted 2085 (DM=1475 (70.7%), PM=610 (29.3%)) PM/DM cases and 10193 sex-age matched controls in the period 2000-2018. Both univariate and multivariate analyses were performed to evaluate the link dysthyroidism and PM/DM. Survival analysis was also performed.

The rate of hyperthyroidism was significantly (P=.0097) higher in cases (n=40, 1.9%) with respect to controls (n=123, 1.2%). Similarly, the rate of hypothyroidism was significantly (P<.0001) associated with cases (n=234, 11.2%) when compared to controls (n=853, 8.4%). At the multivariate logistic regression analysis, both DM (OR 1.31 [95%CI 1.07-1.60], P=.0087) and PM (OR 1.54 [95%CI 1.21-1.95], P=.004) were significantly associated with hypothyroidism, whereas DM (OR 1.70 [95%CI 1.10-2.61], P=.0165) but not PM (OR 1.45 [0.83-2.55], P=.1947) was found to be associated with hyperthyroidism. Subjects with PM and positive for anti-Sjögren's syndrome-related antigen A (SSA) auto-antibody displayed a significant risk of developing hyperthyroidism (OR 5.85 [95%CI 1.02-33.74], P=.0480), whereas individuals with DM and positive for antinuclear antibody (ANA) had a higher risk of developing hyperthyroidism (OR 2.65 [95%CI 1.00-7.03], P=.0498).

Physicians treating PM/DM patients should consider screening for thyroid dysfunction on a regular basis.
Physicians treating PM/DM patients should consider screening for thyroid dysfunction on a regular basis.Although public debates on cannabis are ongoing in many countries, there are currently no EU-wide rules for either medical or recreational use of cannabis. Numerous studies have illustrated that creating such rules is a complex challenge. The battle over the legalisation of medical use of cannabis in Poland is a good example for analysing the mutual impact of the social practices that various actors perform through and by the discourses in the field of health care how they discursively create their own roles and take positions and how they shape the coalitions and opposition in trying to achieve their strategic goals. This article aims to reconstruct and explain how different types of knowledge are discursively used to sustain or question power relations in the healthcare field. The dispersed knowledge-power influences social actors, who try to define or redefine social practices and aspire to guide them in the healthcare field. The presented approach allows authors to go beyond the social actors' perception vs institutional regulations and to analyse discursive actions as elements of the complex networks of meanings activating various type of resources. Those dynamic networks, involving the different (and sometimes the same) actors in the different actions, open the new fields of rationality. Starting by identifying the crucial actors and their discursive actions visible in the mainstream press discourse, we will reconstruct three types of groups related to medical cannabis. We will then investigate three types of dispositives - law, discipline and security - to investigate the relations between discursive and non-discursive elements. The proposed analysis belongs to the Critical Discourse Analysis (CDA) paradigm and includes investigation of the media discourses and in-depth interviews with the identified actors.
Assessment of image quality directly in clinical image data is an important quality control objective as phantom-based testing does not fully represent image quality across patient variation. Computer algorithms for automatically measuring noise in clinical computed tomography (CT) images have been introduced, but the accuracy of these algorithms is unclear. This work benchmarks the accuracy of the global noise (GN) algorithm for automatic noise measurement in contrast-enhanced abdomen CT exams in comparison to precise reference noise measurements. The GN algorithm was further optimized compared to the previous report in the literature.

Reference values of noise were established in a public image dataset of 82 contrast-enhanced abdomen CT exams. Nazartinib EGFR inhibitor The reference noise values were obtained by manual regions-of-interest measurements of pixel standard deviation in the liver parenchyma according to an instruction protocol. Noise measurements taken by six observers were averaged together to improve reference noisn of automatic clinical image quality measurement methods. The GN algorithm was optimized and validated for automatic measurement of soft-tissue noise in abdomen CT exams.
The performance of automatic noise measurement was benchmarked in a large clinical CT dataset. The study provides a framework for thorough validation of automatic clinical image quality measurement methods. The GN algorithm was optimized and validated for automatic measurement of soft-tissue noise in abdomen CT exams.A 44-year-old lady, a follow-up case of idiopathic dilated cardiomyopathy and cardiac resynchronization therapy defibrillator device implantation with epicardial left ventricular (LV) lead, underwent a transvenous LV lead revision in view of epicardial lead malfunction. A chest X-ray after this, done for worsening dyspnea, revealed pneumopericardium along with left pneumothorax. The computed tomography (CT) revealed a communication between the left pleural and pericardial cavities, around the old epicardial lead. Drainage of the left pleural cavity resolved both the pneumothorax and pneumopericardium and the patient remained well on follow up.This case report describes a procedure of retrieval of a leadless transcatheter pacemaker from the right pulmonary artery 3 days after device implantation. An 80-year-old man affected by ischemic cardiomyopathy and sinus node dysfunction was implanted with a leadless transcatheter pacemaker in the low septum of the right ventricle. Three days after the procedure, the leadless pacemaker migrated into a side branch of the right pulmonary artery. The device was successfully removed using two snares hooked to a tine via the proximal retrieval feature.
Toxoplasmosis is a zoonotic disease that affects humans and warm-blooded animals. This study describes an outbreak of toxoplasmosis in howler monkeys (Alouatta sp.) and survival of capuchins (Sapajus apella), under the same environmental conditions.

Howler monkeys were submitted to post-mortem examination. Tissue samples were processed to histopathology and immunohistochemistry to detect lesions and tachyzoites of Toxoplasma gondii. Tissue samples were also frozen and submitted to PCR and genotyping of T. gondii.

Typical lesions were observed in several organs including the liver, lymph node, and brain, with intralesional cysts and tachyzoites of T. gondii demonstrated by immunohistochemistry. T. gondii genomic sequences were amplified by PCR, and genotyping characterized the same T. gondii clone in all howler monkeys.

Our results support the notion that some species of neotropical primates are highly susceptible to toxoplasmosis and the hypothesis that capuchins (S. apella) may be resistant.
Our results support the notion that some species of neotropical primates are highly susceptible to toxoplasmosis and the hypothesis that capuchins (S. apella) may be resistant.Killer cell immunoglobulin-like receptors (KIRs) interact with polymorphic human leucocyte antigen (HLA) class I molecules, modulating natural killer (NK) cell functions and affecting both the susceptibility and outcome of immune-mediated diseases. The KIR locus is highly diverse in gene content, copy number and allelic polymorphism within individuals and across geographical populations. To analyse currently under-represented Asian and Pacific populations, we investigated the combinatorial diversity of KIR and HLA class I in 92 unrelated Malay and 75 Malaysian Chinese individuals from the Malay Peninsula. We identified substantial allelic and structural diversity of the KIR locus in both populations and characterized novel variations at each analysis level. The Malay population is more diverse than Malay Chinese, likely representing a unique history including admixture with immigrating populations spanning several thousand years. Characterizing the Malay population are KIR haplotypes with large structural variants present in 10% individuals, and KIR and HLA alleles previously identified in Austronesian populations. Despite the differences in ancestries, the proportion of HLA allotypes that serve as KIR ligands is similar in each population. The exception is a significantly reduced frequency of interactions of KIR2DL1 with C2+ HLA-C in the Malaysian Chinese group, caused by the low frequency of C2+ HLA. One likely implication is a greater protection from preeclampsia, a pregnancy disorder associated with KIR2DL1, which shows higher incidence in the Malay than in the Malaysian Chinese. This first complete, high-resolution, characterization of combinatorial diversity of KIR and HLA in Malaysians will form a valuable reference for future clinical and population studies.
To investigate the impact of strong magnetic fields on the stem-effect in plastic scintillation detectors (PSDs) using Monte Carlo methods.

Prior to building the light guide model, the properties of the Čerenkov process in GEANT4 were investigated by simulating depth-dose and depth-Čerenkov emission profiles in water as functions of Čerenkov process input parameters. In addition, profile simulations were performed for magnetic field strengths ranging from 0T to 1.5T. A PMMA light guide was constructed in GEANT4 using data from the manufacturer and literature. Simulations were performed with the model as functions of depth and fiber-beam angle where the simulated stem-effect spectrum and the Čerenkov light ratio (CLR) were scored and compared to measured data in the literature. The light guide optical properties were iteratively adjusted until agreement between the simulated and measured data was achieved. Simulations were performed with the validated model as functions of depth and magnetic field strength and the simulated data were compared to measured data in the literature.
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