Notes

Unloading the Psychological Health of Nursing staff during COVID-19: Proof via Pakistan.
of intersite radiomics harmonization is crucial before interpreting these features for pathological inference. Level of Evidence 3. Technical Efficacy Stage 1. MGCD0103 J. MAGN. RESON. IMAGING 2021;53394-407.Mannose-6-phosphate (M6P) is recognized by the mannose-6-phosphate receptor and plays an important role in the transport of cargo to the endosomes, making it an attractive tool to improve endosomal trafficking of vaccines. We describe herein the assembly of peptide antigen conjugates carrying clusters of mannose-6-C-phosphonates (M6Po). The M6Po's are stable M6P mimics that are resistant to cleavage of the phosphate group by endogenous phosphatases. Two different strategies for the incorporation of the M6Po clusters in the conjugate have been developed the first relies on a "post-assembly" click approach employing an M6Po bearing an alkyne functionality; the second hinges on an M6Po C-glycoside amino acid building block that can be used in solid-phase peptide synthesis. The generated conjugates were further equipped with a TLR7 ligand to stimulate dendritic cell (DC) maturation. While antigen presentation is hindered by the presence of the M6Po clusters, the incorporation of the M6Po clusters leads to increased activation of DCs, thus demonstrating their potential in improving vaccine adjuvanticity by intraendosomally active TLR ligands.We evaluated the safety and feasibility of adipose-derived mesenchymal stem cells to treat endoscopically urinary incontinence after radical prostatectomy in men or female stress urinary. We designed two prospective, nonrandomized phase I-IIa clinical trials of urinary incontinence involving 9 men (8 treated) and 10 women to test the feasibility and safety of autologous mesenchymal stem cells for this use. Cells were obtained from liposuction containing 150 to 200 g of fat performed on every patient. After 4 to 6 weeks and under sedation, endoscopic intraurethral injection of the cells was performed. On each visit (baseline, 1, 3, 6, and 12 months), clinical parameters were measured, and blood samples, urine culture, and uroflowmetry were performed. Every patient underwent an urethrocystoscopy and urodynamic studies on the first and last visit. Data from pad test, quality-of-life and incontinence questionnaires, and pads used per day were collected at every visit. Statistical analysis was done by Wilcoxon signed-rank test. No adverse effects were observed. Three men (37.5%) and five women (50%) showed an objective improvement of >50% (P  less then  .05) and a subjective improvement of 70% to 80% from baseline. In conclusion, intraurethral application of stem cells derived from adipose tissue is a safe and feasible procedure to treat urinary incontinence after radical prostatectomy or in female stress urinary incontinence. A statistically significant difference was obtained for pad-test improvement in 3/8 men and 5/10 women. Our results encourage studies to confirm safety and to analyze efficacy.Bi-allelic HOXA1 pathogenic variants clinically manifest as two distinct syndromes, Bosley-Salih-Alorainy syndrome (BSAS) and Athabascan brainstem dysgenesis syndrome, mainly reported in two different populations from Saudi Arabia and southwest North America, respectively. Here we report two siblings of Indian origin with BSAS phenotype caused by a novel homozygous exon 2 HOXA1 pathogenic variants.
Noninvasive assessment of intracranial stenosis is important to manage ischemic stroke patients. However, few previous studies have compared 3D black-blood MRI with 3D time-of-flight (TOF), magnetic resonance angiography (MRA), and digital subtraction angiography (DSA) for intracranial artery plaque assessment.

To compare 3D black-blood MRI and 3D TOF-MRA, using DSA as the reference standard for intracranial stenosis and atherosclerotic plaque assessment in patients with posterior circulation stroke or transient ischemic attacks (TIAs).

Prospective, cohort study.

One hundred and one patients with posterior circulation stroke and/or TIA (age 63 ± 10 years, 84 male) who underwent DSA and MRI within 4 weeks of each other.

3D fast-spin-echo MRI for intracranial vessel wall imaging (IVWI) and 3D TOF at 3T.

Two radiologists independently measured the degree of stenosis on 3D IVWI and TOF, using DSA as a reference. Plaque enhancement was recorded when the plaque was stenosis-free on DSA.

Shapiro-Wilk'sEvidence 1 Technical Efficacy Stage 2 J. MAGN. RESON. IMAGING 2021;53469-478.
3D black-blood MRI is accurate and reproducible for quantifying intracranial artery stenosis compared with DSA, and performs better than 3D TOF. As compared to DSA, it detects more nonstenotic plaques. Level of Evidence 1 Technical Efficacy Stage 2 J. MAGN. RESON. IMAGING 2021;53469-478.The situation of coronavirus disease 2019 (COVID-19) pandemic is rapidly evolving, and medical researchers around the globe are dedicated to finding cures for the disease. Drug repurposing, as an efficient way for drug development, has received a lot of attention. However, the huge amount of studies makes it challenging to keep up to date with the literature on COVID-19 therapeutic development. This review addresses this challenge by grouping the COVID-19 drug repurposing research into three large groups, including clinical trials, computational research, and in vitro protein-binding experiments. Particularly, to facilitate future drug discovery and the creation of effective drug combinations, drugs are organized by their mechanisms of action and reviewed by their efficacy measured by clinical trials. Providing this subtyping information, we hope this review would serve the scientists, clinicians, and the pharmaceutical industry who are looking at the new therapeutics for COVID-19 treatment.Peroxidase-generated proximity labeling is in widespread use to study subcellular proteomes and the protein interaction networks in living cells, but the development of subcellular RNA labeling is limited. APEX-seq has emerged as a new method to study subcellular RNA in living cells, but the labeling of RNA still has room to improve. In this work, we describe 4-thiouridine (s4 U)-enhanced peroxidase-generated biotinylation of RNA with high efficiency. The incorporation of s4 U could introduce additional sites for RNA labeling, enhanced biotinylation was observed on monomer, model oligo RNA and total RNA. Through the s4 U metabolic approach, the in vivo RNA biotinylation efficiency by peroxidase catalysis was also dramatically increased, which will benefit RNA isolation and study for the spatial transcriptome.Lithium-sulfur (Li-S) batteries, despite having high theoretical specific energy, possess many practical challenges, including lithium polysulfide (LiPS) shuttling. To address the issues, here, hydrophilic molybdenum boride (MoB) nanoparticles are presented as an efficient catalytic additive for sulfur cathodes. The high conductivity and rich catalytically active sites of MoB nanoparticles allow for a fast kinetics of LiPS redox in high-sulfur-loading electrodes (6.1 mg cm-2 ). Besides, the hydrophilic properties and good wettability toward electrolyte of MoB can facilitate electrolyte penetration and LiPS redox, guaranteeing a high utilization of sulfur under a lean-electrolyte condition. Therefore, the cells with MoB achieve impressive electrochemical performance, including a high capacity (1253 mA h g-1 ) and ultralong lifespan (1000 cycles) with a low capacity fade rate of 0.03% per cycle. Also, pouch cells fabricated with the MoB additive deliver an ultrahigh discharge capacity of 947 mA h g-1 , corresponding to a low electrolyte-to-capacity ratio of about 4.8 µL (mA h)-1 , and remain stable over 55 cycles under practically necessary conditions with a low electrolyte-to-sulfur ratio of 4.5 µL mg-1 .
To evaluate the color stability of CAD/CAM complete denture resins.

A total of 176 resin specimens were manufactured from conventional heat-polymerizing (pink CON
n = 16; tooth-shade CON
n = 16), CAD/CAM subtractively manufactured (pink WI
n = 16, AV
n = 16, ME
n = 16, PO
n = 16; tooth-shade AV
n = 16, ME
n = 16, PO
n = 16), and additively manufactured (pink ND
n = 16; tooth-shade ND
n = 16) denture resins; four different aging processes (thermal cycling, distilled water, red-wine, and coffee) were used. A spectrophotometer evaluated the color change (ΔE) using two modes of measurements (specular component included (ΔE
) and specular component excluded (ΔE
)) recorded at baseline (T
) and at day#30 (T
). ANOVA and post hoc tests were used for statistical analysis (alpha = 0.05).

Additively manufactured resins (ND
and ND
) demonstrated significant ΔE in comparison to the other groups in all aging media (p < 0.001). WI
demonstrated higher ΔE
in comparison to the other subtractively manufactured groups in distilled water (p < 0.001). In red-wine, AV
revealed significantly more ΔE
than PO
(p = 0.039). In coffee, the ΔE
was higher for CON
than ME
(p = 0.026) and PO
(p = 0.011). Similarly, in coffee the ΔE
for AV
was higher than PO
(p = 0.030).

Additively manufactured denture resins demonstrated the maximum color change compared to conventional heat-polymerized and CAD/CAM subtractively manufactured denture resins. Furthermore, CAD/CAM subtractively manufactured denture resins were not inferior to conventional resins in terms of color stability.
Additively manufactured denture resins demonstrated the maximum color change compared to conventional heat-polymerized and CAD/CAM subtractively manufactured denture resins. Furthermore, CAD/CAM subtractively manufactured denture resins were not inferior to conventional resins in terms of color stability.The structure and packing of organic mixed ionic-electronic conductors have an especially significant effect on transport properties. In operating devices, this structure is not fixed but is responsive to changes in electrochemical potential, ion intercalation, and solvent swelling. Toward this end, the steady-state and transient structure of the model organic mixed conductor, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOTPSS), is characterized using multimodal time-resolved operando techniques. Steady-state operando X-ray scattering reveals a doping-induced lamellar expansion of 1.6 Å followed by 0.4 Å relaxation at high doping levels. Time-resolved operando X-ray scattering reveals asymmetric rates of lamellar structural change during doping and dedoping that do not directly depend on potential or charging transients. Time-resolved spectroscopy establishes a link between structural transients and the complex kinetics of electronic charge carrier subpopulations, in particular the polaron-bipolaron equilibrium. These findings provide insight into the factors limiting the response time of organic mixed-conductor-based devices, and present the first real-time observation of the structural changes during doping and dedoping of a conjugated polymer system via X-ray scattering.Diabetes mellitus is a serious worldwide metabolic disease, which is accompanied by hyperglycaemia and affects all organs and body system. Zinc (Zn) is a basic cofactor for many enzymes, which also plays an important role in stabilising the structure of insulin. Liver is the most important target organ after pancreas in diabetic complications. In this study, we aimed to investigate the protective role of Zn in liver damage in streptozotocin (STZ)-induced diabetes mellitus. There are four experimental groups of female Swiss albino rats group I control; group II control + ZnSO4 ; group III STZ-induced diabetic animals and group IV STZ-diabetic + ZnSO4 . To induce diabetes, STZ was injected intraperitoneally (65 mg/kg). ZnSO4 (100 mg/kg) was given daily to groups II and IV by gavage for 60 days. At the end of the experiment, rats were killed under anaesthesia and liver tissues were collected. In the diabetic group, hexose, hexosamine, fucose, sialic acid levels, arginase, adenosine deaminase, tissue factor activities and protein carbonyl levels increased, whereas catalase, superoxide dismutase, glutathione-S-transferase, glutathione peroxidase, glutathione reductase and Na+ /K+ -ATPase activities decreased.
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