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Transition-Metal-Free C2-Functionalization of Pyridines through Aryne Three-Component Direction.
Evolutionary characteristics of sex-biased genes indicated throughout cricket minds and gonads.
Transmission electron microscopy showed the formation of autophagosomes and vesicles. Pectolinarigenin also caused arrest of the SK-HEP-1 cells at the G2/M-phase of the cell cycle. Wound healing and transwell assays showed pectolinarigenin suppressed the migration and invasive potential of the SK-HEP-1 cells. CONCLUSIONS The present study revealed that pectolinarigenin exhibits antitumor activity in SK-HEP-1 liver cancer cells via multiple mechanisms and may prove promising in the development of systemic therapy for liver cancer.PURPOSE To evaluate the effect of transcatheter arterial chemoembolization (TACE) under the guidance of contrast-enhanced ultrasound (CEUS) in patients with advanced hepatocellular carcinoma (HCC). METHODS One hundred and sixty patients with HCC admitted to Cangzhou Central Hospital from April 2015 to April 2017 were enrolled. The clinical data were retrospectively analyzed. Seventy-five patients who underwent TACE according to CEUS results were selected as the observation group. The remaining 85 cases that underwent digital subtraction augiography (DSA) angiography-guided TACE were selected as the control group and were intravenously infused with 15 mg of Endostar+500 mL of normal saline once a day for 3 consecutive days (Endostar 30 mg was reperfused during the operation). Both groups were re-contrasted at 1 month (T2) and 3 months (T3) to determine whether TACE was performed again. The numbers of TACEs re-performed were recorded. Color Doppler energy imaging was used to observe the neovascularization of thvely inhibit tumor angiogenesis, control tumor progression, and prolong the survival of patients, which is conducive to the prognosis of patients.PURPOSE Leukemia causes annually a significant number of deaths. The main objective of this study was to investigate the anticancer properties of piperine and curcumin against HL60 leukemia cells and to elucidate the underlying mechanism. METHODS Antiproliferative effects were assessed by WST-1 cell viability assay. Cell apoptotic effects were studied by DAPI staining assay. Annexin V/propidium iodide (PI) assay was used to assess apoptosis. Electron microscopy was used for detection of autophagy and flow cytometry for cell cycle analysis, while transwell migration assay was used to study the effects on cell migration and invasion. Protein expression was estimated by western blot. RESULTS The results showed that both piperine and curcumin inhibited significantly the growth of the HL60 cells and exhibited an IC50 of 25 and 30 µM respectively. Further, it was observed that the anticancer effects of piperine and curcumin were due to the induction of mitochondrial-mediated apoptosis which was also associated with enhancement of the Bax expression. Transmission electron microscopy also showed that both curcumin and piperine induced autophagy in the HL-60 leukemia cells. Flow cytometry showed that piperine and curcumin also caused arrest of the HL-60 cells at the S-phase of the cell cycle. Finally wound healing and transwell assays showed that piperine and curcumin suppressed the migration and invasive potential of the HL60 cells. CONCLUSIONS The present study reveals that piperine and curcumin exhibit significant antitumor activity in human leukemia HL60 cells via multiple mechanisms and may prove promising in the development of systemic therapy for leukemia.PURPOSE This study aimed to investigate the expression and the prognostic value of CIP2A in multiple myeloma (MM). METHODS The expression levels of CIP2A was measured in 33 newly diagnosed MM patients (at presentation and after 4 cycles of Bortezomib-Dexamethazone (BD) regimen) and 15 healthy controls by real time quantitative polymerase chain reaction (QRT-PCR). RESULTS CIP2A expression was upregu¬lated in MM patients compared to controls. There was a significant reduction in CIP2A expression after treatment with BD regimen. Patients with expression levels ≤ 16.45 EU (expression unit) were more likely to respond to BD regimen (23 patients out of 23) than those with expression level >16.45 (6 patients out of 10) (p=0.005). learn more learn more Lower progression-free survival (PFS) (16.7%) was observed among patients with high CIP2A expression levels compared to 50% PFS in patients with lower CIP2A expression levels (p=0.006). CONCLUSION CIP2A is upregulated in MM and bortezomib downregulated its expression. High CIP2A level is associated with shorter PFS and poor response to BD in MM. Therefore, beside its value as a poor prognostic indicator in MM, CIP2A suppression might be a fruitful future targeted therapeutic agent aiming to improve the outcome in MM.PURPOSE The current study aimed at investigating the anticancer effects of plant-based flavone Baicalein against the thyroid cancer. METHODS Cell viability was assessed using MTT assay. Flow cytometric-based estimation of cell cycle analysis was done for determining the cancer cell phase distribution. DAPI staining method followed by fluorescent microscope examination was used for inferring the cancer cell apoptosis. Transmission electron microscopy (TEM) was used for detection of autophagosomes. Western blotting was done for protein concentration estimation. RESULTS Baicalein induced dose-dependent decline in proliferation of MDA-T68 thyroid cancer cells, while the reduction of cell proliferation was surprisingly lower for normal thyrocytes. IC50 of 10μM was estimated against cancer cells. Baicalein induced cell apoptosis in a concentration-dependent manner. Induction of apoptosis was attributed to increase in apoptotic protein concentration and signal was mediated through change Bax/Bcl-2 ratio. The autophagic cell death occurred in cancer cells when treated with Baicalein. The mechanism of cell death was inferred as modulation of NF-kB signaling pathway. Baicalein was also seen to induce mitotic cell cycle arrest in thyroid cancer cells by reducing the concentration of Cyclin B1 mitotic protein. CONCLUSION The results of current study suggest Baicalein as an important anticancer agent against thyroid cancer. Future research to further investigate and enhance the effects of Baicalein against thyroid cancer is needed.
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