Notes

Management of key hands dystonia: current status.
Numerous long non-coding RNAs (lncRNAs) are reported to affect the progression of multiple myeloma (MM). This study is aimed to explore the role and downstream mechanism of lncRNA LINC01003 in MM.

Xenograft tumor assay was used to assess the function of LINC01003 in MM in vivo. The mRNA expression levels of LINC01003, miR-33a-5p, and PIM1 were determined by quantitative real-time polymerase chain reaction. Cell viability was examined by MTT assay. Relative protein levels of apoptosis-related factors (Bcl-2 and Bax) and proviral integration site of the Moloney leukemia virus kinase 1 (PIM1) were detected via western blot. Adhesion-related proteins were measured by Enzyme-linked immunosorbent assay was used to determine the levels of adhesion-related proteins. Besides, the target relation among LINC01003, miR-33a-5p and PIM1 was tested via dual-luciferase reporter assay.

Low expression of LINC01003 was observed in MM cell lines and peripheral blood samples of MM patients. Both LINC01003 up-regulation and miR-33a-5p down-regulation repressed cell viability and adhesion, and promoted apoptosis of MM cells. Moreover, LINC01003 suppressed the growth of xenograft tumor in mice. We then identified miR-33a-5p as a downstream target of LINC01003, and confirmed that PIM1 was a direct target gene of miR-33a-5p. Both high expression of miR-33a-5p and low expression of PIM1 reversed the suppressive effects of LINC01003 overexpression on cell adhesion and viability, and the promoting effect on apoptosis in MM cells.

LINC01003 functioned as a sponge of miR-33a-5p to inhibit the development MM by regulating PIM1 expression.
LINC01003 functioned as a sponge of miR-33a-5p to inhibit the development MM by regulating PIM1 expression.
Neutralization tests (NT) are the gold standard for detecting and quantifying anti-SARS-CoV-2 neutralizing antibodies (NAb), but their complexity restricts them to research settings or reference laboratories. Antibodies against S protein receptor binding domain (RBD) have been shown to confer a neutralizing activity against SARS-CoV-2. Assays quantitatively measuring anti-S1-RBD-SARS-CoV-2 antibodies could be of great value for NAb screening of potential donors for convalescent-phase plasma therapy, assessing natural or vaccine-induced immunity, stratifying individuals for vaccine receipt, and documenting vaccine response.

Elecsys Anti-SARS-CoV-2 S (Elecsys-S), a high-throughput automated electrochemiluminescence double-antigen sandwich immunoassay for quantitative measurement of pan-anti-S1-RBD-SARS-CoV-2 antibodies, was evaluated against NT on 357 patients with PCR-confirmed SARS-CoV-2 infection. NT was performed in a BSL-3 laboratory using a Slovenian SARS-CoV-2 isolate; the NT titer ≥120 was considere candidates for convalescent-phase plasma donors, selected commercial anti-SARS-CoV-2 assays with optimized cutoff, like Elecsys-S, could be used for rapid, automated, and large-scale screening of individuals with clinically relevant NAb levels as suitable donors.Metastasis is the major reason for most brain tumors with up to a 50% chance of occurrence in patients with other types of malignancies. Brain metastasis occurs if cancer cells succeed to cross the 'blood-brain barrier' (BBB). Moreover, changes in the structure and function of BBB can lead to the onset and progression of diseases including neurological disorders and brain-metastases. Generating BBB models with structural and functional features of intact BBB is highly important to better understand the molecular mechanism of such ailments and finding novel therapeutic agents targeting them. Hence, researchers are developing novel in vitro BBB platforms that can recapitulate the structural and functional characteristics of BBB. Brain endothelial cells-based in vitro BBB models have thus been developed to investigate the mechanism of brain metastasis through BBB and facilitate the testing of brain targeted anticancer drugs. Bioengineered constructs integrated with microfluidic platforms are vital tools for recapitulating the features of BBB in vitro closely as possible. In this review, we outline the fundamentals of BBB biology, recent developments in the microfluidic BBB platforms, and provide a concise discussion of diverse types of bioengineered BBB models with an emphasis on the application of them in brain metastasis and cancer research in general. We also provide insights into the challenges and prospects of the current bioengineered microfluidic platforms in cancer research.White matter (WM) alterations have been identified as a relevant pathological feature of Huntington's disease (HD). Increasing evidence suggests that WM changes in this disorder are due to alterations in myelin-associated biological processes. Multi-compartmental analysis of the complex gradient-echo MRI signal evolution in WM has been shown to quantify myelin in vivo, therefore pointing to the potential of this technique for the study of WM myelin changes in health and disease. This study first characterized the reproducibility of metrics derived from the complex multi-echo gradient-recalled echo (mGRE) signal across the corpus callosum in healthy participants, finding highest reproducibility in the posterior callosal segment. Subsequently, the same analysis pipeline was applied in this callosal region in a sample of premanifest HD patients (n = 19) and age, sex and education matched healthy controls (n = 21). In particular, we focused on two myelin-associated derivatives i. the myelin water signal fraction vo evidence supporting myelin breakdown as an early feature of HD.Biodegradable polymers have been regarded as a promising solution to tackle the pollutions caused by the wide use of conventional polymers. However, during the biodegradation process, the material fragmentation leads to microplastics. In this work, the formation of microplastics from biodegradable poly (butylene adipate-co-terephthalate) (PBAT) in different aquatic environments was investigated and compared with the common non-biodegradable low-density polyethylene (LDPE). Proteasome inhibitor The results showed that a much larger quantity of plastic fragments/particles were formed in all aquatic environments from PBAT than from LDPE. In addition, UV-A pretreatment, simulating the exposure to sunlight, increased the rate of PBAT microplastic formation significantly. The size distribution and shapes of the formed microplastics were systematically studied, along with changes in the polymer physicochemical properties such as molecular weight, thermal stability, crystallinity, and mechanical properties, to reveal the formation process of microplastics. This study shows that the microplastic risk from biodegradable polymers is high and needs to be further evaluated with regards to longer timeframes, the biological fate of intermediate products, and final products in freshwater, estuarine and seawater natural habitats. Especially, considering that these microplastics may have good biodegradability in warmer 20 - 25° water but will most likely be highly persistent in the world's cold deep seas.Methane is a type of renewable fuel that can generate many types of high value-added chemicals, however, besides heat and power production, there is little methane utilization in most of the wastewater treatment plants (WWTPs) all round the world currently. In this review, the status of methane production performance from WWTPs was firstly investigated. Subsequently, based on the identification and classification of methane oxidizing bacteria (MOB), the key enzymes and metabolic pathway of MOB were presented in depth. Then the production, extraction and purification process of high value-added chemicals, including methanol, ectoine, biofuel, bioplastic, methane protein and extracellular polysaccharides, were introduced in detail, which was conducive to understand the bioconversion process of methane. Finally, the use of methane in wastewater treatment process, including nitrogen removal, emerging contaminants removal as well as resource recovery was extensively explored. These findings could provide guidance in the development of sustainable economy and environment, and facilitate biological methane conversion by using MOB in further attempts.This paper aims to present a new quantitative systematic approach to evaluate the effectiveness of utilizing and allocating resources based on the concept of "Work" in physics. This method is examined in the Urmia Lake Basin (ULB), shrinking of which has threatened the life of about five million inhabitants and ecosystem biodiversity. In the proposed approach, the role of three types of financial, human, and environmental resources in the development process is evaluated quantitatively, and they have been compared in two periods before and after the severe reduction of the lake water volume. Results show that although financial resources have increased by 1.9 times in the second period, the effectiveness of the development process has decreased. Therefore, the resources have not been utilized properly in a direction compatible with sustainable development strategies. Additionally, the improperly-spent financial resources on the development projects especially in the second period have had a more destructive role than the human and environmental resources in the ULB crisis.Life cycle assessment and kinetic modeling were used to elucidate the impact of thermal intensification (TI) on resource consumption and the techno-economic feasibility of a Fenton process at laboratory scale. Increasing temperature from 25 to 55 °C lowers treatment time (96.5%) and electricity use (67.8%) due to the positive impact of temperature on the reaction rate; however, beyond 50 °C no significant diminution in energy use, emissions, and operating cost was observed. The environmental footprint of the process is linked with energy use, operating pH, and the electricity share of the country; nevertheless, the impact of transport and infrastructure materials was lower. At 55 °C and pH of 2.8, emissions of precursors of freshwater and marine eutrophication, particulate matter formation, and ionizing radiation were reduced more than half; besides, in most of the midpoint categories, pondered by the ReCiPe-2016 method, emissions were lowered ca. 43.3%. The endpoint categories human health, ecosystem quality, and resource availability had a significant decline in disability-adjusted life years (46.0%), time-integrated species loss (42.0%), and surplus cost (33.1%). Harnessing the energy present in the wastewater itself decreased 41.9% global warming potential (GWP), but the use of steam for heating raised it 718.8%. In countries where electricity generation is dependent on fossil fuels, GWP could increase (2.0-20.0%) whereas GWP would decrease (8.8-9.4%) when renewable energy sources dominate. Operating at 55 °C and pH of 5.5 rose the reaction time (1835.5%), GWP (29.3%), particulate matter formation (44.3%), terrestrial acidification (21.8%), marine (48.9%), and freshwater eutrophication (66.7%). TI of Fenton processes could increase their treatment capacity with a small reduction in the quality of the effluent; furthermore, they can be made affordable for low-to-medium scale industries in emerging economies due to decreased resources consumption and emissions, leading to a lower treatment cost (US$0.49/m3).
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