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Portrayal of the Total Mitochondrial Genome of Basidiomycete Candida Hannaella oryzae: Intron Advancement, Gene Rearrangement, and its particular Phylogeny.
A total of eight metabolites and fourteen conjugates in the reactive pathways of hydrolysis, substitution, reduction, methylation, glycosylation, acetylation, and malonylation were characterized by HRLC-Q-TOF-MS/MS. The relationship between the metabolized derivatives of FSA and enhanced expression the corresponding enzymatic regulators was established. This study will help understand the mechanisms and pathways of FSA metabolism and inspire the further research on FSA degradation in the paddy crops and environmental or health risks.Chlordane is an organochlorine pesticide that is applied extensively. Residual concentrations that remain in soils after application are highly toxic to soil organisms, particularly affecting the earthworm gut and indigenous soil microorganisms. However, response mechanisms of the earthworm gut and indigenous soil microorganism communities to chlordane exposure are not well known. In this study, earthworms (Metaphire guillelmi) were exposed to chlordane-contaminated soils to investigate their response mechanisms over a gradient of chlordane toxicity. Results from high-throughput sequencing and network analysis showed that the bacterial composition in the earthworm gut varied more significantly than that in indigenous soil microbial communities under different concentrations of chlordane stress (2.3-60.8 mg kg-1; p less then 0.05). However, keystone species of Flavobacterium, Candidatus Nitrososphaera, and Acinetobacter remained stable in both the earthworm gut and bacterial communities despite varying degrearthworm gut and indigenous soil bacteria in resisting chlordane stress and sustaining microbial equilibrium in soil.Excessive nitrate (NO3-) in rivers can lead to water quality deterioration, and can also be directly input into estuaries and oceans, thus posing a serious threat to the stability of their ecosystems. In this study, the concentration, isotopes and sources of NO3- in 30 rivers discharging into the Bohai Sea were comprehensively investigated. The mean concentration of NO3--N was 2.24 ± 2.11 mg L-1, with obvious seasonal and spatial variations. In total, 104.24 kt of NO3--N was discharged into the Bohai Sea annually, to which the Yellow River Basin and Liao River Basin made the largest contributions. The range of δ15N-NO3- was -1.1‰ to +33.2‰ (mean value, +11.4 ± 5.0‰), with no significant seasonal or spatial differences; the mean value of δ18O-NO3- was +9.4 ± 7.2‰, with much higher values seen in June. Based on the MixSIAR model, manure (24.3 ± 7.5%) and sewage (19.1 ± 14.5%) were the primary sources of NO3- in the 30 rivers, followed by NO3- fertilizers (16.3 ± 12.5%), soil N (15.5 ± 11.9%), atmospheric deposition of NO3- (13.5 ± 5.7%) and NH4+ fertilizers (11.4 ± 8.9%). This finding highlights the vital roles of sewage and manure management in riverine NO3-. Using a mathematical method, the contributions of various sources to each river were simulated. The results indicated that management of the Yellow River, Daliao River, Liao River, and Xiaoqing River is more urgently needed than that of other rivers to control Bohai NO3- pollution. We believe that this finding will provide guidance for scientific management of NO3- pollution in these 30 rivers and the Bohai Sea.It is important to evaluate how ambient particles are deposited in the human respiratory system in view of the adverse effects they pose to human health. Traditional methods of investigating human exposure to ambient particles suffer from drawbacks related either to the lack of chemical information from particle number-based measurements or to the poor time resolution of mass-based measurements. To address these issues, in this study, human exposure to ambient particulate matter was investigated using single particle analysis, which provided chemical information with a high time resolution. Based on single particle measurements conducted in the Pearl River Delta, China, nine particle types were identified, and EC (elemental carbon) particles were determined to be the most dominant type of particle. In general, the submicron size mode was dominant in terms of the number concentration for all of the particle types, except for Na-rich and dust particles. On average, around 34% of particles were deposited in the human respiratory system with 13.9%, 7.9%, and 12.6% being distributed in the head, tracheobronchial, and pulmonary regions, respectively. The amount of Na-rich particles deposited was the highest, followed by EC. The overall deposition efficiencies of the Na-rich and dust particles were higher than those of the other particle types due to their higher efficiencies in the head region, which could be caused by the greater sedimentation and impaction rates of larger particles. In the head region, the Na-rich particles made the largest contribution (30.5%) due to their high deposition efficiency, whereas in the tracheobronchial and pulmonary regions, EC made the largest contribution due to its high concentration. In summary, the findings of this initial trial demonstrate the applicability of single particle analysis to the assessment of human exposure to ambient particles and its potential to support traditional methods of analysis.Cadmium (Cd) and lead (Pb) pollution in soil and their accumulation in edible parts possess a worldwide eco-environmental and health risk, especially in developing countries. Recently, organosilicone fertilizer (OSiF) has been reported to reduce uptake of heavy metals, but the effectiveness has not been verified and its associated mechanisms are not fully understood. This work investigated whether and how OSiF and mineral silicon fertilizer (MSiF) affect mitigation of Cd and Pb stress in rice (Oryza sativa). Both soil incubation and pot experiments were conducted to assess the effect of OSiF and MSiF on bioavailability of Cd and Pb in soil and their accumulation in rice. Gefitinib nmr Additionally, a hydroponic experiment was conducted to study whether Si in rice can alleviate Cd stress. We found that both Si fertilizers could increase soil pH, induce the transformation of the acid soluble and reducible fractions of Cd and Pb to the oxidizable and residual fractions in soil, decreasing their bioavailability and the uptake of Cd and Pb in rice.
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