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Leukoaraiosis and intense ischemic cerebrovascular accident.
Triclosan, a broad-spectrum antimicrobial agent, which is widely used in several pharmaceutical and personal care products, has been known to pose adverse impacts on various groups of aquatic organisms, including fish. The present study aimed to compare the sensitivity of five selected freshwater fishes toward triclosan exposure. The acute toxicity or median lethal concentration (LC50-96 h) of triclosan determined using probit analysis was 1.76 mg L-1 in Anabas testudineus, 448 µg L-1 in Danio rerio, 700 µg L-1 in Gambusia affinis, 1.35 mg L-1 in Oreochromis mossambicus, and 750 µg L-1 in Pseudetroplus maculatus. Based on the length-weight relationship, the lowest condition factor, or K value, was recorded in Danio rerio (0.931) while the highest value was observed in Anabas testudineus (2.343) indicating negative allometric growth. Similarly, the weight of fishes and gill weight declined significantly (p  less then  0.05) in their respective LC50-96 h groups with concomitant alterations in the rate of oxygen consumption denoting respiratory distress. Triclosan exposure also resulted in the modification of fish behavior and histomorphological damage in gill tissues. The alterations in all parameters tested were more prominent in Danio rerio and thus considered the most sensitive species, while Anabas testudineus comparatively tolerated the toxicant to some extent. The study provides the baseline data that variation in the length-weight relationship is one of the major factors that influences the toxic effects of triclosan on the selected freshwater fishes.
Primary function of regulatory T(Treg) cells is to control and regulate the immune responses. In many patients with tumor tissues, increased Treg cell numbers have been reported. In this study, we aimed to measure the cellular content of blood samples in patients with gastric cancer (GC) and define their role in tumor progression.

We prospectively evaluated 34 gastric cancer cases and 20 healthy control samples. The blood was collected from both the gastric coronary and peripheral veins of the patients and only from the peripheral vein of the control group. Cellular content and lymphocyte subset including, regulatory T cells, were determined by flow cytometric analysis.

The GC patients revealed similar percentages of T cells, B cells, neutrophils, and eosinophils in the venous samples from periphery vein and gastric coronary. learn more The percentage of monocytes from the tumor-draining gastric coronary vein was significantly lower than monocytes from the peripheral vein in gastric cancer patients (p=0.03). T-regulatory cells had a higher percentage in samples obtained from gastric cancer patients compared with the control group.

Our findings confirmed that patients with gastric cancer have a significantly higher percentage of regulatory T cells than the control group, suggesting that they may contribute to the tumor progress. Regulatory T cells and monocytes interact in patients with GC, which can be used as a parameter in the clinical follow-up of patients with GC.

Gastric cancer, Treg cell, Flow cytometry, FoxP3.
Gastric cancer, Treg cell, Flow cytometry, FoxP3.Network meta-analysis is an extension of standard meta-analysis. It allows researchers to build a network of evidence to compare multiple interventions that may have not been compared directly in existing publications. With a Bayesian approach, network meta-analysis can be used to obtain a posterior probability distribution of all the relative treatment effects, which allows for the estimation of relative treatment effects to quantify the uncertainty of parameter estimates, and to rank all the treatments in the network. Ranking treatments using both direct and indirect evidence can provide guidance to policy makers and clinicians for making decisions. The purpose of this paper is to introduce fundamental concepts of Bayesian network meta-analysis (BNMA) to researchers in psychology and social sciences. We discuss several essential concepts of BNMA, including the assumptions of homogeneity and consistency, the fixed and random effects models, prior specification, and model fit evaluation strategies, while pointing out some issues and areas where researchers should use caution in the application of BNMA. Additionally, using an automated R package, we provide a step-by-step demonstration on how to conduct and report the findings of BNMA with a real dataset of psychological interventions extracted from PubMed.
To evalue the short-term results obtained in endovenous laser ablation (EVLA) procedure of the varicose insufficiency of the lower limbs with Diode 1470 nm laser compared to Diode 1940 nm laser.

A total of 55 patients were enrolled in the study. The patients were divided into two groups those subjected to 1470 nm laser treatment in group A and those with 1940 nm laser treatment in group B. The endpoints were Closure of the target vessel, complications and post-operative pain.

There are no intra-and post-operative complications. The occlusion rate of the target veins was 100% at 7- and 60-day controls. The pain perceived in the immediate post-operative and at the controls showed no statistically significant differences between the two groups. However in group B it was necessary to apply lower values of Power (W) and Linear Energy Density (LEED) with a statistically significant difference compared to group A.

Short-term results demonstrate closure rates comparable to those obtained with 1470 nm lasers. There were no statistically significant differences in the two groups in terms of primary and secondary endpoints. The advantage of using 1940 nm laser technology is that it is possible to dispense a lower linear energy density (LEED) at a lower power (Watt).

Endovenous laser ablation with Diode 1940 nm is particularly suitable and advantageous in the treatment of superficial and small vessels, as well as venous segment adjacent to nerve structures.

Diode laser, Intravenous ablation, Varicose, Venous insufficiency, Veins.
Diode laser, Intravenous ablation, Varicose, Venous insufficiency, Veins.Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance. With cell barcoding and microchip technologies, a suite of high-throughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at single-cell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories. Microchip technologies including microfluidics- and microwell-based platforms play a major role in high-throughput scRNA-seq. As the emerging technology, spatial transcriptomics integrates cellular transcriptomics with their spatial coordinates within tissues for spatially deciphering cellular composition, heterogeneity and cell-cell communications. Spatial transcriptomics has been increasingly recognized as one of the most powerful tools for discovering new biology and advancing precision medicine. Microfluidics as an enabling technology plays an increasingly important role in spatial transcriptomics. We review the technological spectrum and advances in high-throughput scRNA-seq and spatial transcriptomics, discuss their advantages and limitations, and pitch into new biology learned from these new tools.
This study was designed to compare the incidence of nursing related complications in patients undergoing coronary intervention through either the radial or femoral artery and to analyze the advantages and disadvantages of the two nursing approaches.

Between September 2012 and June 2017, 360 patients underwent coronary intervention in radiology department and were enrolled in this study, 196 of these patients being assigned to the radial artery group and 164 of them to the femoral artery group. The postoperative adverse reactions and complications in these two groups were observed and recorded, and the results of the two groups were compared using a chi-square test and logistic regression.

The incidences of limb pain, restlessness and insomnia, low back pain, urine retention, bleeding and subcutaneous hematoma were significantly lower in the radial artery group than in the femoral artery group, but the rate of shifting approach or giving-up intervention due to artery spasm or deformity was significantly higher than that in the femoral artery group. However, the differences in the incidence of aneurysms and compartment syndrome between the two groups were not statistically significant.

Compared with the femoral artery approach, the radial artery approach results in less trauma, fewer complications, and shorter bed rest time. It also puts less psychological pressure on patients, and is more easily accepted by them. Finally, it means simpler nursing, and, thus, it should be widely promoted.

Complications, Coronary intervention, Femoral artery, Nursing, Radial artery.
Complications, Coronary intervention, Femoral artery, Nursing, Radial artery.
Preoperative evaluation of axillary lymph node status is very important for the choice of primary treatment in breast cancer. This study assessed predictive properties of positron emission tomography-computerized tomography for axillary lymph node metastasis.

Patients who were operated between 2014-2019 for early breast cancer and who had preoperative positron emission tomography-computerized tomography images were included. Positron emission tomography-computerized tomography results and histopathologic results were compared retrospectively.

Medical records of 223 patients who were operated for early breast cancer and have had positron emission tomography-computerized tomography were evaluated. positron emission tomography-computerized tomography revealed that axillary lymph node status is positive in 97 patients and negative in 126 patients. In histopathological examination, tumor cells were found in 86 patients, of those 27 were negative while 59 were positive in positron emission tomography-computerized tomography. Furthermore, 137 patients were free from tumor cells, in which 99 were negative and 38 were positive in positron emission tomography-computerized tomography. Positive and negative predictive value of positron emission tomography-computerized tomography for metastatic axillary lymph nodes were found 60.8% and 78.5%, respectively.

Clinically, positron emission tomography-computerized tomography has low negative predictivity for determination of axilla in early stage or operable breast cancer. Even though it is valuable for the systematic scanning, positron emission tomography-computerized tomography can be overlooked for axillary evolution.

Axilla, Breast Cancer, Cancer Staging, PET-CT.
Axilla, Breast Cancer, Cancer Staging, PET-CT.The production of water-dispersed graphene with low defects remains a challenge. The dry ball milling of graphite with additives produces edge-selectively functionalized graphene. However, the "inert" additives require a long milling time and cause inevitable in-plane defects. Here, the mechanochemical reaction of graphite with persulfate solved the above drawback and produced edge-selectively hydroxylated graphene (EHG) nanosheets through a 2 h ball-milling and a subsequent 0.5 h sonication. The mechanochemical cleavage of persulfate yielded SO4 ⋅- to spontaneously oxidize graphite to form the carbon radical cations selectively at edges, followed by hydroxylation with water of moisture. Because the O-O bond dissociation energy of persulfate is 20 % of the graphitic C-C bond, the rather low milling energy allowed the hydroxylation of graphite at edges with nearly no in-plane defects. The obtained EHG showed high water-dispersibility and excellent photothermal and electrochemical properties, thereby opening up a new door to fabricate graphene-based composites.
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