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Genetic engineering took up speed in the 70s
Take DNA from a frog and cut it up in pieces
Take gene that encodes certain proteins
(restriction enzymes "cut" in different places)
cut up piece of DNA
add frog DNA to cut bacterial plasmid
DNA Ligase acts like tape or other adhesive
take the new plasmids and put them into other bacteria
penetrate holes of e. coli cell membrane
put plasmids into e. coli cells
put e.coli in agar/tetracycline plates
gelbox separates DNA
result: lots of copies of the gene we want
yay insulin and stuff
boo paper beards
(Seriously. Don't quit your day job, MIT.)
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