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Welcome to Nembutal Euthanasia With Dignity Home. Females not receiving sexual experience were injected with the hormone regimen and remained in their home cages in the colony room. At 4-5 hr after the administration of progesterone, an adult male hamster that had received sexual experience through use in other sexual behavior studies was placed in the home cage of the experimental female. The females were housed individually, and male stimulus animals were housed in groups of three or four in plastic cages (50.8 × 40.6 × 20.3 cm). The order of the cages containing the males was rotated each week to minimize the likelihood that an individual male and female were paired more than once during the 6 weeks of sexual experience. Male and female Syrian hamsters were delivered from Charles River Laboratories (Kingston, NY) at ∼60 d of age. Immunocytochemistry. Female hamsters, killed with an overdose of sodium pentobarbital, were intracardially perfused with a 25 mm PBS solution, pH 7.5, for 2 min (flow rate, 25 ml/min), followed by 4% paraformaldehyde in PBS for 20 min. They include, for instance, water-absorbing and water-insoluble celluloses such as crystalline cellulose, cellulose, α-cellulose, and cross-linked sodium carboxymethyl cellulose; water-absorbing and water-insoluble starches such as hydroxypropyl starch, carboxymethyl starch, cross-linked starch, amylose, amylopectin and pectin; water-absorbing and water-insoluble proteins such as gelatin, casein, sodium and casein; water-absorbing and water-insoluble gums such as gum arabic, tragacanth gum and glucomannan; and cross-linked vinyl polymers such as cross-linked polyvinyl pyrrolidone, cross-linked carboxyvinyl polymer or its salt, cross-linked polyvinyl alcohol and polyhydroxyethylmethacrylate.  Post　was generated  by G᠎SA Con tent G ener ator DEMO .

Sexual experience. Approximately 1 week after the females arrived at the laboratory, they were bilaterally ovariectomized under sodium pentobarbital (Nembutal) anesthesia (8.5 mg per 100 gm body weight, i.p.). On the day of the experience test, the females received 500 μg of progesterone in 0.1 ml of cottonseed oil (subcutaneous injection). This sequence was repeated a second time followed by an intake test on day 12 where all groups had access to sucrose for 15 min in the morning in the absence of ip injections. One group of females received 6 weeks of sexual experience with a stimulus male; the second group remained sexually naive. After ovariectomy, the females were initially divided into two groups. The slices were placed between two mold-release-coated slides and left at 80°C for 48 hr. At ibogaine uk , the slices could be coverslipped in Gelvatol (Harlow and Lane, 1988) or processed for immunohistochemical labeling. Immunohistochemical labeling. Tissue slices were washed in 25 mm Tris-buffered saline, 0.8% NaCl, pH 7.4 (TBS) for 30 min.

The slices were then washed three times for 10 min each in working buffer and then placed in working buffer (4°C) containing 1:100 donkey anti-guinea pig IgG conjugated to Cy5 (Jackson Immuno-Research, West Grove, PA). For examining the extent of GFAP labeling in LY-filled astrocytes, background signal was first removed from the datasets by thresholding, and then the GFAP (Cy5) and LY channels were searched for voxels containing signal from both labels using the Colocalization module in the Bitplane package. Low levels of background signal were removed using a baseline-subtraction threshold. Z-motor calibration was checked using z-series through 15 μm fluorescent latex beads. Semithin (0.5 μm) sections were cut and placed on Formvar-covered slot grids. Grids were poststained with uranyl acetate and Sato lead, coated with 10 and 20 nm gold particles, and carbon coated. When common names are used, substituents on the hydrocarbon chain are designated by Greek letters rather than by numbers, and counting begins not with the carboxyl carbon but with the adjacent carbon.  C ontent　has  been g᠎enerated with the he᠎lp of G SA Con᠎te nt Generator Demov ersi on᠎!

All ranges reported for mean values are the SEM and SD are provided as indications of population variance. Therefore, it is suggested that the prior values of this parameter had a stronger influence on the results of the model. This procedure finds all voxels within a confocal volume containing signal intensities from both channels within a range of user-defined values for each channel, in which voxel dimensions are determined by the pixel size (0.09-0.15 μm) and step size (0.36-0.81 μm). The volume of the astrocyte was then determined by counting voxels containing LY signal. The resulting voxels containing colocalization constituted the volume of GFAP in the dye-filled astrocyte. In this way, the neuropilar volume (volume of neuropil infiltrated by a single astrocyte) of filled astrocytes was determined. The slices were infiltrated with a solution of 50% acetone-50% Durcupan ACM epoxy resin (Electron Microscopy Sciences, Ft. After a 2 min rinse in distilled water, the slices were dehydrated with an ethanolic series (50, 70, 90, and 100%), followed by dry acetone (two times for 40 sec of irradiation per solution).


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