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- 300 uL total volume
- Buffer 150 uL @ 100 mM Potassium Phostphate Buffer @ 7.0 pH, 7.4 pH, and 8.0 pH
- This is put in first
- adenosine 50 uL @ 100 mM
- This is put in second
-DI H20 50 uL
- This is put in third
- 50 uL enzyme some dilution in a linear way at our 3-5 window
-This is put in last
- set the M2 Microplate at a certain temperature. @ ??
Km = 400 uM for bovine spleen
Need to run a BCA Assay on the protein to find the protein content and specific activities (min/mol/mg)
- This will be run on Thursday
- Set up in terms up quartettes (4) to set up the standard curve in triplicate
- figure out dilutions for adenosine deaminase doesn't have to be 10-fold range
400
200
100
50 is in the middle
25
12.5
6.125
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