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Nitrogliserin adalah larutan yang mudah meledak, berminyak, tak berwarna dan berat. Nitrogliserin digunakan juga sebagai bahan aktif pembuatan bahan peledak (kebanyakan dinamit) yang digunakan pada bidang konstruksi, demolisi, dan industri pertambangan. Nitrogliserin digunakan untuk keperluan medis sebagai vasodilator potensial untuk pengobatan jantung seperti angina pektoris (angin duduk) dan gagal jantung kronis, selama lebih dari 130 tahun. Meskipun efek yang menguntungkan ini telah diketahui sejak diketahuinya proses konversi dari nitrogliserin menjadi nitrogen oksida, suatu vasodilator, baru pada tahun 2002 ditemukan enzim yang dapat melakukan proses konversi yaitu aldehyde dehydrogenase mitokondrial. Nitrogliserin tersedia dalam bentuk tablet sublingual, spray, dan potongan kecil.
Bentuk nitrogliserin sendiri ada tiga macam, yaitu intravena, semprotan sublingual, dan lempung.
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