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BOD (American Public Health Association, 1995) was
modified and used as the model wastewater throughout the
study. This modified artificial wastewater (AW) contained
(NH4)2SO4, 0.56 g;
MgSO4·7H2O, 0.20 g;
CaCl2, 15 mg;
FeCl3·6H2O, 1 mg;
MnSO4·H2O, 20 mg;
NaHCO3, 0.42 g;
a trace mineral solution (Diekert, 1991), 10 ml;
phosphate buffer (1 M, pH 7.0), 50 ml;
and distilled water, 940 ml.
The wastewater was autoclaved at 110 ◦C for 1 h before
use. The autoclaved wastewater was added along with
filter-sterilized glucose and glutamic acid solutions. The
BOD of the wastewater was varied from 20 to 200 mg/l.
The artificial wastewater was made and maintained under a
nitrogen atmosphere by connecting to a nitrogen-containing
gas-tight bag with a volume of 15 l (Alltech, Deerfield, IL,
USA), as shown in Fig. 1(A).
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