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- you first need to remove the required gene from another organism's DNA.
-You will also need to take out the plasmid from the agrobacterium tumefaciens and then insert the required gene into the plasmid.
-the plasmid should then be placed back into the agrobacterium.
-the plasmid should be inserted into the plants DNA causing the leaf discs to be infected with the agrobacterium,
-the leaf dics with the new gene would grow into a whole new plant. Each cell of the plant will now contain the new gene.
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