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*lab (Medium) MacConkey agar and Mannitol salt agar
pure culture- medium that contains only a single known species or type of microorganism.
**mixed culture vs contaminated culture vs pure culture
streak culture
*enrichment medium vs general purpose medium and differential medium
*Antonu van Leeuwenhoek (pond and microscope)
**no test questions on microscopes, waves or refraction**
****Tested on types of microscopes, dark-field microscope, Bright-filed microscope and phase contrast microscope ***
*scanning electron microscope vs transmission electron microscope*
**gram stain, Acid-fast stain, endospore stain, capsule stain**
(need to know details about gram stain)
spore stain, capsule stain
GRAM STAIN
does it have thick or thin cell wall
gram -positive bacteria stain PURPLE
gram-
1. stain w/ CV
2. iodine to cover slide CV-I
3. decolorize - E+OH
(+) shrink cell wall - CV slow removal
(-) removes crystal iodine (outer membrane removal) - CV removed faster
4. stain w. safranin
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