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100gVml
40 micromolar
Preparation of FUdR-containing plates: make a stock solution
of FUdR at a concentration 40 mM in dd H2O (by adding
984 mg in 100 mL dd H2O). Filter-sterilize the solution, ali-
quot and freeze at -20°C. Add 3 mL of the stock solution in
1 L NGM agar after it cools down to 55°C, just before pouring
plates.
pretreated with 0.25 mL of
250 µg mL−1 5-fluoro-2′-deoxyuridine (FUDR) and ampicillin
FUDR from a 10 mg/mL aqueous stock was added at
1:100 to PEG-NGM prior to filter-sterilization
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