Notes

Wig、bigwig和bedgraph文件详解_fengleqi的博客

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For customized tracks, use the bedGraphformat if it is necessary to retain exact information when exporting. However, nayawig wigs of all custom tracks is limited. For these reasons, we suggest always changing wiggle files to the bigWig storage format and reference these from your customized tracks or track hubs by way of their URL. Create a bedGraph format file following the instructions here. The bigWig files are in an listed binary format. The main advantage of this format is that only these portions of the file needed to display a selected region are transferred to the Genome Browser server.

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Note that for each variableStep and fixedStep formats, the identical span must be used all through the dataset. If no span is specified, the default span of 1 is used. As the name suggests, fixedStep wiggles require the same measurement step all through the dataset. If not specified, a step dimension of 1 is used. Chromosome positions are specified as 0-relative. The final position in a chromosome of size N can be N - 1.

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Wiggle data must be steady and consist of equally sized elements. If your data is sparse or incorporates parts of various sizes, use the bedGraph format as an alternative of the wiggle format. If you've a really large bedGraph data set, you'll find a way to convert it to the bigWig format using the bedGraphToBigWig program.

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