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samples with ultraviolet–diode array detection (UV–DAD). Successful separation and quantitative determination of
analytes was carried out on LiChrospher 60 RP-select B (250 × 4 mm, 5 μm) analytical column. Mixture of acetonitrile–0.1% formic acid in water (65:35, v/v) was used as a mobile phase, with flow rate of 1 mL/min, constant
column temperature at 25 °C, and UV detection at 220 nm. The target residues were extracted with acetone by
ultrasonication, followed by a cleanup using liquid–liquid extraction (LLE) and solid-phase extraction (SPE). The
obtained values for multiple correlation coefficients (R2 > 0.90), relative standard deviation (RSD) of retention
times, peak areas and heights (RSD ≤ 2.25%), and recoveries ranging from 90.55% to 105.40%, with RSD of
0.02% to 5.37%, revealed that the developed method has a good linearity, precision, and accuracy for all analytes.
Hence, it is suitable for routine determination of investigated fungicides in table grape samples.
Keywords: RP-HPLC, UV–DAD, captan, folpet, metalaxyl, table grape
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