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Nonpolar substances like to mix with other nonpolar substances, and do not like to mix with polar substances.
Water is a very polar molecule.
Indelible ink is a mixture of substances that are significantly less polar than water, and are best separated with a relatively less polar solvent.
Isopropyl alcohol is less polar than water.
In this lab, the stationary phase, the paper is also only partially polar.
When the indelible ink is given a choice of binding to the paper or dissolving in the alcohol solvent, it doesn't have a strong preference either way and so it is
moved along by the alcohol solvent and can separate into its various components and produce a colorful chromatogram.
When indelible ink is placed on a medium and water is allowed to travel across the medium, the ink does not move up the paper and does not separate.
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