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weights of ~150g.
The growing rafts
floating in the tubs were 1.31m above the floors and 1.26m below the light fixtures. Fifty lettuce plants
(5 rows of 10 plants per row) were grown at 30 plants per square meter in rigid Styrofoam rafts of
25mm thickness. Round 25mm diameter holes were spaced at 200mm on center for rockwool plant
plugs, and the rows were staggered for optimal uniformity of light on all sides. Each tub continuously
operated a recirculating pump and two coarse air stones (Sweetwater AS-2s rated at 0.1CFM/stone,
1.9cm × 1.9cm × 3.8cm, Pentair AES, Apopka, FL, USA) to ensure strong mixing and to maintain
dissolved oxygen (DO). The diffusing air stones were confirmed to be flowing vigorously multiple
times weekly. Recirculation pumps (24Lpm) mixed the water in each tub at a rate equal to a hydraulic
retention time of 18 min. Water lost through evapotranspiration or evaporation from the hydroponic
tubs was replaced with the tub’s appropriate treatment solution
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