Notes

Chol-Dex nanomicelles: Combination, characterization and also assessment since effective drug carriers regarding intestines focusing on.
of
(L.) were not toxic in mice at low concentrations, whereas some toxic effects were detected in mice treated at 2000 mg/kg.
The results of the present study indicate that the hydroethanolic extracts of different parts of two varieties of A. pyrethrum (L.) were not toxic in mice at low concentrations, whereas some toxic effects were detected in mice treated at 2000 mg/kg.
Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation medium of ewe oocytes selected based on brilliant cresyl blue (BCB) screening on
maturation (IVM), fertilization, and pre-implantation embryo development.

Cumulus-oocyte complexes (COCs) were obtained from the ovaries of slaughtered ewes by either aspiration or slicing techniques. COCs were
matured in a medium containing IGF-1 and EGF (control group). For BCB screening, oocytes were stained and divided into BCB+ oocytes that matured in the same maturation conditions without adding growth factors (Group 2) or in the presence of growth factors (Group 3), and BCB- oocytes that matured in medium without growth factors (Group 4) or with growth factors (Group 5).

The supplementation of the maturation medium with growth factors during IVM of (BCB+) oocytes resulted in a significant increase in nuclear maturation rate (90.9%), fertilization rate (75.6%), and embryo developmental rates (60.0%, 46.7%, and 33.3% for cleavage, morula, and blastocyst, respectively).

Culturing BCB+ oocytes in a maturation medium containing both EGF and IGF-1 showed a significant improvement in nuclear maturation, fertilization, and pre-implantation embryo development
.
Culturing BCB+ oocytes in a maturation medium containing both EGF and IGF-1 showed a significant improvement in nuclear maturation, fertilization, and pre-implantation embryo development in vitro.
Several free-roaming dogs can be easily found in the public areas of Bali. They go out in search of foods and friends and defecate everywhere. In general, these groups of dogs do not receive good healthcare from their owners and are generally threatened by some disease-causing organisms, especially helminths. This study was conducted to identify and measure the prevalence of soil-transmitted helminths (STHs) that cause infection in free-roaming dogs in Bali Province, Indonesia.

A total of 1611 fresh dog fecal samples were collected from all areas of Bali Province and subjected to qualitative fecal examination using flotation techniques to obtain STH eggs.

The incidence of STHs in free-roaming dogs was 38.36%. However, three types of STHs were identified, including
spp.,
spp., and
spp.
spp. had the highest prevalence of 37.8%, followed by
spp. and
spp. at 6.02% and 0.87%, respectively. Multiple infections of these worms were also recorded. The polyparasitism prevalence of
spp. and
spp. was 3.85%, followed by that of
spp. and
spp. at 0.5% and that of
spp. and
spp. at 0.06%. There were no altitude-wise differences in the prevalence of STH infection.

The prevalence of STHs was high in free-roaming dogs. This finding necessitates more serious attention as it affects both animal and public health.
The prevalence of STHs was high in free-roaming dogs. This finding necessitates more serious attention as it affects both animal and public health.
Antibiotic-resistant infections are one of the leading threats to public health globally. Indiscriminate use of antibiotics in food animal production is an important driver of resistance, particularly among foodborne pathogens such as non-typhoidal
(NTS). While there has been extensive research on antimicrobial-resistant (AMR)
in India and China, there have been few studies in countries in South Asia, including Nepal. This is particularly important with the rise of commercial poultry farming in Nepal as a means of economic development and nutritional subsistence. This descriptive study seeks to identify the prevalence and resistance patterns of NTS serotypes focusing on Chitwan, Nepal's leading poultry producing district.

A mixture of purposive and judgment sampling of 18 poultry farms and 20 slaughterhouses representing a broad geographic distribution across multiple municipalities in Chitwan was conducted in May 2019. Environmental samples taken from poultry farms included Water, litter, feces, few.veterinaryworld.org/Vol.14/February-2021/14.pdf).
NTS is highly prevalent among Chitwan's growing poultry industry with higher rates of positivity found in slaughterhouse samples compared with environmental samples from farms. In addition, a high rate of AMR (78%) was revealed, and an extremely concerning number of those were shown to be MDR (20%). This baseline data has important implications for poultry production and consumption in the region. Further research will elucidate the extent to which this contamination and drug resistance is impacting the health of the local population and help inform treatment and management strategies.Note The characterization of the poultry industry and practices that might be linked to NTS contamination in the Chitwan district are detailed in the previous paper in this series (www.veterinaryworld.org/Vol.14/February-2021/14.pdf).
Poultry is becoming an increasingly important source of protein in the Nepalese diet. The Chitwan region of Nepal is the hub of the emerging poultry industry. Little is known about the prevalence of non-typhoidal
(NTS) on poultry farms or the role of farm management practices that may contribute to the presence of NTS on farms. The role of poultry in the transmission of
to humans is also poorly defined. This descriptive study seeks establish baseline data through estimation of the prevalence of NTS on broiler and layer operations in various farms of the Chitwan district of Nepal.

Based on district documents on poultry production and meat marketing, a purposive sampling of 18 commercial poultry farms comprising ten broilers farms and eight layers farms was conducted. Environmental samples including water, litter, feces, feed, farm, and eggshell swabs were randomly collected from each farm. Samples were cultured and tested for the presence of NTS; positives were serotyped, and antimicrobial susceptibito understanding the epidemiology of zoonotic strain of NTS in the region and are necessary for the design of future studies and mitigation plans and underlines the need for a one-health approach to protect public health-related to Salmonella spp. from poultry farms.
is one of the most common enteric protozoan parasites in vertebrates, such as humans, domestic and wild animals, causing giardiasis. To the best of our knowledge, little is known about the genetic diversity of
assemblages. This study aimed to identify genetic diversity of
assemblages in Iranian stray dogs.

A total of 450 fecal samples were collected from 2015 to 2016 from stray dogs of Northwest Iran. All specimens were observed microscopically following concentration and flotation techniques. Subsequently, DNA samples were extracted, amplified, and sequenced targeting the
gene.

The overall prevalence of
in infected dogs was estimated at 1.6%, based on microscopic and molecular diagnoses. Sequencing and phylogenetic analyses indicated a high level of genetic diversity of assemblage C (haplotype diversity; 0.802).

The pairwise sequence distances between the identified isolates of assemblage C showed an intradiversity of 0.3%-1.3% and identity of 98.7%-100%. Current findings indicate that a significant genetic diversity of
assemblage C haplotypes is unequivocally circulates among stray dogs in Northwest Iran.
The pairwise sequence distances between the identified isolates of assemblage C showed an intradiversity of 0.3%-1.3% and identity of 98.7%-100%. Current findings indicate that a significant genetic diversity of G. duodenalis assemblage C haplotypes is unequivocally circulates among stray dogs in Northwest Iran.
Blood collection from dogs is the most commonly performed procedure in the medical clinic. However, different factors can interfere with the quality of the material collected, potentially causing complications for patients. Simulated skill training is a teaching strategy designed to provide early training to students, develop their skills and self-confidence, and increase the procedure's success while reducing complications. Therefore, the aim of this study was to evaluate skill training using a low-fidelity simulator in the peripheral venipuncture procedure and examine the training's influence on the
procedure.

To assess skill training, this study used a low-fidelity simulator in the peripheral venipuncture procedure and examines the training's effect on the
procedure. In total, 100 dogs, 65 undergraduate students, 3 veterinarians, and 4 previously trained evaluators participated. The canine
venipuncture procedure was evaluated both before and after the simulated skill training and the low-fidelity simulator training. Data were collected on participants' self-confidence levels.

Local complications occurred during
practice; however, after training, they decreased. Gloves were more frequently used during the procedure, resulting in a reduction of both harvest attempts and complications, as well as increased levels of self-confidence in post-training participants. The simulator developed had low fidelity, low cost, and was easy to create.

Skill training in peripheral venipuncture using a low-fidelity simulator positively influences student learning, increases their self-confidence during
harvesting, and reduces the complications of the procedure, improving patient well-being.
Skill training in peripheral venipuncture using a low-fidelity simulator positively influences student learning, increases their self-confidence during in vivo harvesting, and reduces the complications of the procedure, improving patient well-being.
Feline leukemia virus (FeLV) is classified as
. FeLV occurs worldwide, including Malaysia. Thus far, only one decade-old study on molecular characterization of Malaysian FeLV isolates exists, which resulted in a scarcity of updated information of current FeLV isolates circulating in Malaysia. This study was conducted to determine the status of FeLV in clinically ill cats and to study the molecular characterization and phylogenetic relatedness of the current isolates.

Convenience sampling was performed in 20 cats from the Gasing Veterinary Hospital in Selangor. Plasma and saliva samples were collected from 15 clinically ill cats and 5 healthy cats subjected to one-step reverse transcription-polymerase chain reaction with primers targeting a highly conserved gene of U3-LTR-gag.

Two clinically ill cats' plasma and saliva samples tested positive for FeLV RNA. Partial nucleotide sequencing and phylogenetic analysis revealed that the current isolates were 94-99% homologous to the previous Malaysian and Japanese FeLV isolates.

Current FeLV isolates from this study displayed higher similarity with the previous Malaysian isolates, signifying that a similar FeLV strain circulated among the cat population in Selangor.
Current FeLV isolates from this study displayed higher similarity with the previous Malaysian isolates, signifying that a similar FeLV strain circulated among the cat population in Selangor.The anesthetic-surgical stress response consists of metabolic, neuroendocrine, hemodynamic, immunological, and behavioral adaptations through chemical mediators such as the adrenocorticotropic hormone, growth hormone, antidiuretic hormone, cortisol, aldosterone, angiotensin II, thyroid-stimulating hormone, thyroxine, triiodothyronine, follicle-stimulating hormone, luteinizing hormone, catecholamines, insulin, interleukin (IL)-1, IL-6, tumor necrosis factor-alpha, and prostaglandin E-2. Behavioral changes include adopting the so-called prayer posture, altered facial expressions, hyporexia or anorexia, drowsiness, sleep disorders, restriction of movement, licking or biting the injured area, and vocalizations. Overall, these changes are essential mechanisms to counteract harmful stimuli. However, if uncontrolled surgical stress persists, recovery time may be prolonged, along with increased susceptibility to infections in the post-operative period. This review discusses the neurobiology and most relevant organic responses to pain and anesthetic-surgical stress in dogs and cats. It highlights the role of stress biomarkers and their influence on autonomous and demeanor aspects and emphasizes the importance of understanding and correlating all factors to provide a more accurate assessment of pain and animal welfare in dogs and cats throughout the surgical process.
Ultrasonography (USG) is useful for non-invasively identifying changes that occur in soft tissue architecture. The objective of this research was to demonstrate postpartum (PP) uterine involution through the changes of perineal muscle intensity and thickness in Madura beef cow by ultrasonography.

Madura's breed cows used in the research consist of eight non-pregnant (NP) cows and three PP cow. The transrectal and transperineal USG imaging of NP cows was performed on days 1, 33, and 65. USG imaging of PP cows was performed every day starting from day 1 (24 h after parturition) until day 21 PP. Transrectal USG of the reproductive tract was performed for the cervix, corpus uteri, and cornua uteri. USG was performed transcutaneously over the perineal area for coccygeus and levator ani muscles at the longitudinal and transverse angles. Reproductive tract diameter and perineal muscle intensity and thickness were measured with ultrasound imaging.

The analysis of the sonogram of PP cows showed that the diameterof PP cows (5.8 mm increased to 6.5 mm and 3.8 mm increased to 4.8 mm, respectively) and NP cows (8.8 mm increased to 9.1 mm and 5.9 mm decreased to 4.9 mm, respectively).

We found that the perineal muscles, namely, the levator ani muscle and coccygeus muscle, change in size, and intensity during uterine involution as demonstrated on Madura beef cow.
We found that the perineal muscles, namely, the levator ani muscle and coccygeus muscle, change in size, and intensity during uterine involution as demonstrated on Madura beef cow.
Backyard pigs farming is a rearing system associated with poor hygienic and sanitary conditions of the pig, often causing public health and food safety problems. Therefore, this study was conducted to investigate the occurrence of potentially zoonotic parasites in population pig reared under backyard farming in the Bucaramanga metropolitan area, Northeast Colombia.

From September to December 2019, a total of 558 fecal samples from 64 backyard pig farms were examined for the presence of enteric protozoan infection. The coprological diagnosis was done by direct examination using Lugol's iodine solution, buffered saline solution, and Kinyoun technique. In addition, blood samples were collected from 200 pigs. Serum was collected and used for the detection of
and
cysticercosis infections, using the enzyme-linked immunosorbent assay.

The overall prevalence of zoonotic protozoa in the Bucaramanga metropolitan area was 65.2%, reporting 52.7% prevalence for
, 33.7% for
, and 5.7% for
spp. Regarding tozoan infections and porcine cysticercosis reported in this study could be due to poor facilities, and lack of hygiene in the facilities, and suggests the possible transmission of these parasite populations between pigs and humans, thus increasing the transmission of parasites zoonotic potential. Therefore, appropriate sanitary management practices and deworming programs should be adopted to reduce the prevalence of these infectious agents.This review article delineates the role of chelated minerals and phytogenic feed additives (PFAs) cocktail supplementation in improving the overall health status and production performance of poultry birds and its economic effects in the poultry industry. Organically complexed minerals have many advantages over inorganic sources. It has improved absorption and efficacy, which meets the bird's requirements comfortably with a low-dose level. Hence, inorganic forms can be replaced with lower-dose levels of organic minerals without any adverse effects on production performances in broilers and layers. PFAs possess medicinal properties, such as antimicrobial, antioxidant, adaptogenic, and immunomodulatory, therefore, could be recommended as supplements. They are also growth promoters that enhance the overall health status and augment poultry birds' production performance. Furthermore, the tremendous potential of PFAs could be extracted with the recent advances in science and technology. With the advantages of organically complexed minerals and multiple beneficial applications, there is a resurgence to develop PFAs as a cocktail of organic minerals to improve the overall health status of poultry birds and augment their productivity, which, in turn, helps the poultry industry to grow decisively and economically.
is a bovine intestinal nematode. Immune pictures following infection are conflicting and stopping anthelmintic albendazole treatment recording reversed liver abnormalities. The purpose of this work was to evaluate the therapeutic potential of bone marrow mesenchymal stem cells (BMMSCs) therapy, subsequent to albendazole administration in rats infected with
.

The ultrasonographic and histopathological examinations as well as serum liver enzymes activity and the kinetics of recovery were investigated. The correlation of cell-mediated and humoral immune pictures was assessed by assaying immunoglobulins, splenocytes viability, phagocytic index, and Th1/Th2 cytokines.

The cultured BMMSCs counting were 4.21×10
cells/cm
with 96.03% viability. Flow-cytometric analysis indicated positive CD90 (82%), CD105 (79%) and negative CD34 (0.37%), CD45 (0.42%), attesting to the suitability of the isolated BMMSCs for use in therapy. Transplantation of BMMSCs after albendazole administration significantly reduced thenimize obstacles in using stem cell therapy in animal toxocariosis.
The final results indicated that the liver functions, histopathological findings, and immune parameters were aggravated after experimental T. vitulorum infection. Albendazole treatment followed by BMMSCs therapy was found to assist in regeneration of injured hepatic tissue. Besides, it appeared to modulate host defensive immune responses against T. vitulorum antigens. This work could define more clearly the events that manipulate the host immune, histopathological, and biochemical responses to minimize obstacles in using stem cell therapy in animal toxocariosis.
Goats are reared for their meat, mohair and other socio-cultural needs in Lesotho. Helminth infections are some of the major setbacks in the goat production industry due to their negative impact on animals' health, resulting in significant losses on meat and mohair production and death. A cross-sectional study was conducted to determine the prevalence, fecal egg infestation, and morphological identification of gastrointestinal parasites in goats.

Fecal samples were collected from 765 goats and subjected to McMaster egg counting techniques using the flotation method. Statistical analyses were performed using the Statistical Package for the Social Sciences (SPSS v.26.0).

The overall prevalence of gastrointestinal parasites was 94.7%, and the identified gastrointestinal parasites were nematodes (64.7%), coccidia (25.8%), and cestodes (4.2%).
was identified as the prevalent gastrointestinal nematode species found in goats. The prevalence and fecal egg count of gastrointestinal parasites were significantly higher (p<0.05) in goats located in the highlands and Senqu River Valley, while goats in the lowlands demonstrated a significantly (p<0.05) higher prevalence of
. Immature goats and kids were more significantly (p<0.05) prone to gastrointestinal parasites.

The nematodes and coccidia infestations were prevalent in goats located in the highlands and foothills, respectively, whereas nematode and coccidia fecal egg loads were higher in goats located in the foothills and Senqu River Valley, respectively.
The nematodes and coccidia infestations were prevalent in goats located in the highlands and foothills, respectively, whereas nematode and coccidia fecal egg loads were higher in goats located in the foothills and Senqu River Valley, respectively.
Postpartum uterine infectious diseases, such as pyometra, have discrepancy with both health and, subsequently, productivity of dairy cows due to its high prevalence and the high cost of treatment. Therefore, this study investigates the influence of pyometra on the reproductive indices, the metabolic profile, and oxidant/antioxidant parameters of the pyometric animal compared to those of healthy ones.

The study included 30 cows. The animals were differentiated into two groups of 15 cows each A group of pyometra and a control group. All pyometric cows were subjected to breeding soundness examination after the end of pyometra and were compared to the control group. Blood samples were obtained to assess the levels of glucose, non-esterified fatty acids (NEFA), triglycerides (TGs), cholesterol, albumin, total protein, alanine aminotransferase, aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine, calcium (Ca), phosphorus, sodium, potassium, progesterone hormone (Ptive for oxidative stress. Overall, checking the energy balance, metabolic imbalances, and oxidant/antioxidant profile, accompanied with pre-emptive procedures during the postpartum period, is essential and can reduce the chances of such diseases and possible noxious results in highly productive cows.
The reproductive indices was adversely influenced in cows with postpartum pyometra, and metabolic profile, involving energy balance signals and liver function indicators, revealed differences between the two groups. Increased levels of oxidative stress parameters and decrease levels of antioxidant levels were also found, suggesting that pyometra is an incentive for oxidative stress. Overall, checking the energy balance, metabolic imbalances, and oxidant/antioxidant profile, accompanied with pre-emptive procedures during the postpartum period, is essential and can reduce the chances of such diseases and possible noxious results in highly productive cows.Although the production of safe food for human consumption is the primary purpose for animal rearing, the environment and well-being of the animals must also be taken into consideration. Based on microbiological point of view, the production of healthy food from animals involves considering foodborne pathogens, on the one hand and on the other hand, the methods used to fight against germs during breeding. The conventional method to control or prevent bacterial infections in farming is the use antibiotics. However, the banning of these compounds as growth promoters caused many changes in animal breeding and their use has since been limited to the treatment and prevention of bacterial infections. In this function, their importance no longer needs to be demonstrated, but unfortunately, their excessive and abusive use have led to a double problem which can have harmful consequences on consumer health Resistance to antibiotics and the presence of antibiotic residues in food. The use of probiotics appears to be a suitable alternative to overcome these problems because of their ability to modulate the immune system and intestinal microflora, and further considering their antagonistic role against certain pathogenic bacteria and their ability to play the role of growth factor (sometimes associated with prebiotics) when used as feed additives. This review aims to highlight some of the negative effects of the use of antibiotics in animal rearing as well as emphasize the current knowledge on the use of probiotics as a feed additive, their influence on animal production and their potential utility as an alternative to conventional antibiotics, particularly in poultry, pig, and fish farming.
Zinc has a wide spectrum of biological activities and its deficiency has been related to various dysfunctions. This study aimed to clarify the clinical, hematological, and biochemical changes in Holstein dairy calves with naturally occurring hypozincemia before and after treatment.

This study was carried out on 25 Holstein dairy calves <1 month of age in the El-Salhya Dairy Farm, Al-Sharqiya Province, Egypt. Calves were born from apparent healthy dams without any clinical signs of zinc deficiency. They were divided into two groups. The first group (G1) included five clinically healthy calves that were used as controls. The second group (G2) included 20 calves suffering from alopecia and skin lesions. The diseased calves were then treated by oral administration of zinc oxide at the rate of 80 mg/day for 10 successive days and then 20 mg/week for 2 weeks (G3). A total of 90 samples, whole blood and serum samples were collected during the study across all groups. Whole blood was evaluated for complete blolterations were improved by oral administration of zinc oxide.
Zinc deficiency naturally occurring in calves caused clinical, hematological, and biochemical alterations such as alopecia, skin abnormalities, and macrocytic normochromic anemia. In addition, zinc deficiency altered the cell-mediated immunity as indicated by leukopenia and lymphopenia. These alterations were improved by oral administration of zinc oxide.
Previous studies recorded the prevalence of gastrointestinal nematodes (GIN) in Limpopo Province. However, the studies did not address the seasonal patterns of infection and did not cover all districts of Limpopo Province, namely; Capricorn, Sekhukhune, Waterberg, Mopani, and Vhembe. It is, therefore, important to provide up to date information on the prevalence and seasonal occurrence data of GIN in all districts of Limpopo province. The present study was conducted to determine the occurrence of anthelmintic resistance (AR) and document the prevalence of GIN infecting sheep in five districts of Limpopo Province, South Africa.

Forty animals in each district were used for fecal egg count reduction test (FECRT) to determine AR against ivermectin (0.2 mg/kg), levamisole (LEV) (5 mg/kg), and albendazole (7.5 mg/kg). Egg hatch test (EHT) was used to determine AR against thiabendazole (TBZ) and micro-agar larval development test (MALDT) was used for both TBZ and LEV. Naturally, infected sheep (n=780) were samplthelmintic use. The detection of AR in Limpopo is an important finding because it will help in outlining effective management systems against GIN.
AR recorded in Limpopo Province may be due to under-dosing caused by lack of weighing equipment and high treatment frequencies due to lack of proper training on anthelmintic use. The detection of AR in Limpopo is an important finding because it will help in outlining effective management systems against GIN.Platelet indices are inexpensive, easily accessible parameters and potentially useful prognostic indicators in sepsis. In this study we explore the differences in platelet indices and their kinetics between sepsis survivors and non-survivors. A retrospective cohort-study of 97 cases of culture-positive sepsis at a tertiary-care center in North India. Demographics, clinical and laboratory parameters at admission were assessed. Platelet count (PLT), mean-platelet-volume (MPV), platelet-distribution-width (PDW) and plateletcrit (PCT) on admission, and third, fifth and last days of hospitalization were analyzed. Fractional change in platelet indices (ΔMPV72h, ΔPDW72h, ΔPCT72h, and ΔPLT72h) by day-3 were calculated. Unpaired and paired t-tests were used to compare survivors with non-survivors, and to study the change in platelet indices with time. Logistic regression was used for multivariate analysis. ROC-curves and optimum cut-offs to predict mortality were obtained. There were 64 survivors. Non-survivors had significantly higher ΔMPV72h, ΔPDW72h, day-1 MPV and PDW, and lower ΔPLT72h. MPV and PDW increased, and PLT decreased with time among non-survivors. Trends were reversed in survivors. Only MPV and PDW showed significant change by day-3. Both were independent predictors of mortality on multivariate analysis, alongside ΔMPV72h and ΔPLT72h. On ROC analysis, MPV, PDW, ΔMPV72h, ΔPDW72h and ΔPLT72h effectively predicted mortality. Cut-off for MPV was 10.25 fL (sensitivity = 93.9%, specificity = 60.9%), and PDW, 12.6% (sensitivity = 84.8%, specificity = 51.6%). A rise in MPV and a fall in PLT was associated with mortality in this study. MPV and PDW values at admission are effective predictors of mortality and may be used in conjunction with traditional parameters.[This corrects the article on p. 67 in vol. 37, PMID 32606493.].
It has been known that the "atypia of undetermined significance (AUS)/follicular lesion of undetermined significance (FLUS)" category is the most problematic category in Bethesda system due to its highly heterogeneous morphological features. Recently, it has been reported that aspirates including nuclear atypia in the AUS/FLUS category have a higher risk of malignancy.

This study aimed to assess each nuclear property in aspirates with cytological atypia and also to determine the relationship with the risk of malignancy.

We reviewed 980 AUS/FLUS fine-needle aspirations (FNAs) performed between '2012 and 2019' at a single institution. We classified these aspirates into four groups AUS-N (nuclear atypia), AUS-A (architectural atypia), AUS-H (Hurthle cell change), and AUS-O (other). Nuclear features were detailed sub-classified; size and shape (enlargement, elongation, and overlapping), membrane irregularities (irregular contours, grooves, pseudoinclusion), and chromatin characteristics (pale chromatin). Thincreases the estimated risk of malignancy, all nuclear properties are not equally effective in predicting malignancy risk. Emphasizing nuclear atypia details in reports of AUS case may be a more sensitive way to identify nodules with a high risk of malignancy.
Virus infection has a unique morphological change in cell pathology. It is also of great significance in the diagnosis of herpes simplex mastitis.

To evaluate the practicability and feasibility of cytological diagnosis of herpes simplex virus mastitis.

Three cases of HSV mastitis diagnosed by cytology smears were collected. The papules were stained with Wright-Giemsa to observe the morphological characteristics of the cells.

The cytological characteristics of 3 cases were summarized and compared with the literature.

In the background of inflammatory cells in the smear, the cells with characteristic changes can be seen in the mass and scattered distribution. The nuclear size increased significantly, and mononuclear, binuclear, trinuclear and multinuclear nuclei appeared. The nuclei of multinuclear were crowded and mosaic. The chromatin of nucleus changed like frosted glass, and the chromatin became thicker at the edge of nucleus. Inclusion bodies were found in the nucleus, eosinophilic, with different sizes and irregular shapes. These characteristics are helpful to distinguish from other lesions.

Scraper cytology is a diagnostic tool with simple operation, small trauma and pain, and it has important application value in the differential diagnosis of HSV infection, which deserves the attention of clinicians.
Scraper cytology is a diagnostic tool with simple operation, small trauma and pain, and it has important application value in the differential diagnosis of HSV infection, which deserves the attention of clinicians.
The incidence of thyroid cancer has been increasing worldwide. Thyroid imaging reporting and data system (TIRADS) has been proposed for risk stratification of thyroid nodules to improve categorical management. Fine needle aspiration cytology based on Bethesda system for reporting of thyroid cytopathology (BSRTC) plays a fundamental role in the evaluation of thyroid nodule microscopically. Both the systems, the TIRADS and the latest revised BSRTC 2017, are widely recommended and practiced all over the world, but the correlation between the two systems has not been established.

This study was conducted to assess the risk of malignancy (ROM) in the intermediate Bethesda categories of thyroid lesions and their correlation with the corresponding TIRADS categories.

It was a prospective cross-sectional study over 1 year including 69 patients aged 18 years or older having solitary thyroid nodules. All cases were triaged using both TIRADS and BSRTC 2017 and the diagnostic performances were compared with subsequent paraffin sections to evaluate ROM. Correlation between TIRADS and BSRTC systems was expressed as kappa value.

Good concordance was observed between TIRADS and BSRTC systems in the evaluation of benign thyroid nodule lesions (category 2-II). There was discordance in follicular lesions (category 4-IV). The kappa value generated (0.411) revealed moderate agreement between the two risk stratification systems.

Careful application of both grading systems is essential for the proper segregation of thyroid nodules to facilitate effective clinical and surgical management. However, universally acceptable protocols need to be developed to avoid the heterogeneous approach.
Careful application of both grading systems is essential for the proper segregation of thyroid nodules to facilitate effective clinical and surgical management. However, universally acceptable protocols need to be developed to avoid the heterogeneous approach.
Diagnostic radiation is reported to cause significant damage in buccal cells, while the same effects after natural cell turn over cycle were not checked for in previous studies. The buccal cells were studied in patients exposed to computed tomography (CT) scans for evaluating the cells with micronuclei and cytotoxic changes, namely, pyknotic cells, karyorrhectic cells and karyolytic cells. The pre-exposure counts were compared with postexposure counts on 10 and 20 days corresponding to first and second cell turnover cycles.

The aim of this study is to estimate the counts of micronucleus and cytotoxic changes in buccal cells post-exposure to CT scans and report on variance of the same with first and second buccal cell turnover cycles.

This is an observational study, wherein the buccal smears of patients undergoing CT scans were made before and after CT scan exposures as needed. Papanicolaou (PAP) staining and analysis were performed as per standard criteria for micronuclear and cytotoxic changes, respectively. Statistical test used was paired
-tests.

The micronuclear counts revealed 0.4% positive cells before exposure and 1.4% positive cells post 10 days and 20 days of exposure were significant (
< 0.005). The cytotoxic changes showed around 2.5% positive cells before and 5.7% positive cells 10 days after CT exposure (
< 0.005). The cytotoxic cell values from baseline to 20
day were not significant (
< 0.25).

CT scans have caused genotoxic effects notable after two cell turnover cycles but the cytotoxic changes have significantly decreased naturally after 2
cell turnover as per our study.
CT scans have caused genotoxic effects notable after two cell turnover cycles but the cytotoxic changes have significantly decreased naturally after 2nd cell turnover as per our study.
Wuchereriasis is a significant cause of chronic morbidity. It can affect any organ/tissue in the body. Fine-needle aspiration cytology (FNAC) is an easy method for its detection. A comprehensive analysis of the various facets involved has not been discussed in detail in any publication.

A twenty-six year (February 1994 to January 2020) retrospective audit of all patients who were cytologically diagnosed with wuchereriasis was performed. Data regarding age, sex, organ/tissue involved, and presence of co-existing disease were noted. Hematoxylin and eosin (H and E) and May-Grünwald-Giemsa (MGG) stained slides were screened for microfilaria, adult worm, larval forms, microfilaria ghosts, epithelioid cell granuloma, and eosinophils.

Audit yielded 19,323 cases of which 110 had wuchereriasis giving an incidence of 0.57%. The 11-30 year age group accounted for 41.8% cases. Male female ratio was 1.041. Duration of disease at presentation ranged from 3 days to 24 years. Lymph node was the commonest site involved and inexpensive means of detecting wuchereriasis and is preferred over histopathology. All stages of development of this nematode in human beings are identified in cytology. Microfilaria ghost is a useful clue in screening. The presence of granuloma and eosinophilic infiltrate indicates tissue reaction only. Patients with asymptomatic microfilaraemia should be reported in cytology as they merit treatment.
Molecular testing of thyroid FNA has been advocated in the indeterminate categories of The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) 2018. The utility of cytoscrapes of thyroid FNA samples for
V600E and
mutations was evaluated in this pilot study.

Thyroid FNA samples between 2015 and 2018 from TBSRTC categories 3-6 were included. DNA was extracted from one to two representative smears (cytoscrape). Real-time PCR for
V600E and
(
,
, and
) gene mutations was performed. Histopathology correlation was available in 44 cases.

Chi-square test and calculation of sensitivity, specificity, and positive/negative predictive values were performed.

A total of 73 thyroid FNA cases and 11 nodal metastases of papillary thyroid carcinoma (PTC) were evaluated. The DNA yield ranged from 1.9 to 666 ng/μl (mean 128 ng/μl) in 80 cases and was insufficient in four cases. Overall, mutations were seen in 45 (56.25%) cases with
V600E,
,
, and
in 21 (46.7%), 19 (42.2%), 4, and 1 cases, respectively.
V600E mutation was seen in PTC (11/18, 61%), nodal PTC metastases (5/10, 50%), and occasionally in TBSRTC category 3 (1/18, 5.5%).
mutations were seen across all categories and were maximum in the AUS/FLUS group (6/18, 33%).
V600E /RAS testing had an overall sensitivity, specificity, and positive and negative predictive values of 61.7%, 80%, 91.3%, and 38%, respectively, for the detection of malignancy. In indeterminate thyroid nodules, the sensitivity, specificity, PPV, and NPV were 56.2%, 80%, 81.8%, and 53.3%, respectively.

V600E/RAS mutation testing from cytoscrapes are useful as a rule-in test for indeterminate thyroid nodules and provide molecular confirmation in nodal metastases of PTC.
BRAF V600E/RAS mutation testing from cytoscrapes are useful as a rule-in test for indeterminate thyroid nodules and provide molecular confirmation in nodal metastases of PTC.
The unanimous method of screening cervical cancer is a cervical smear stained with Papanicolaou stain. However, in spite of the various modifications, the staining procedure takes 20 min and uses substantial amount of alcohol which is highly priced. The aim of the study was to assess and analyze the quality of staining of cervical smears stained with Rapid Economical Acetic acid Papanicolaou (REAP) as compared to conventional pap stain in order to establish REAP as an alternative to conventional pap stain.

In this prospective study, a total of two smears each were collected from 50 females who visited the gynecology outpatient department. One of the smears was stained with conventional pap and the other with REAP. The conventional pap and REAP smears were evaluated and compared for the quality of staining.

The cervical smears stained with REAP showed optimal cytoplasmic and nuclear staining in 86% and 90% cases, respectively. The cytological findings and diagnosis of REAP stained smears correlated with their corresponding smears stained with conventional pap in 96% of cases. The turnaround time and cost per smear was much low for REAP as compared to conventional pap stain.

The present study was able to establish REAP as an appropriate alternative to conventional pap stain. The staining by REAP was comparable to conventional pap stain.
The present study was able to establish REAP as an appropriate alternative to conventional pap stain. The staining by REAP was comparable to conventional pap stain.
Numerous pathological processes involve synovium and periarticular tissues that are characteristic and in some cases specific to a particular disease. Synovial fluid is a thick, stringy fluid found in the cavity of synovial joint. Examination of the synovium plays a key role in the diagnosis of many joint diseases.

The aim of this study was to study the spectrum of synovial lesions on fine needle aspiration cytology (FNAC) and to compare the cytological diagnosis with histopathological findings.

This retrospective diagnostic analytical study was carried out in the Department of Pathology. Sixty-six patients with suspected synovial lesions referred from other departments who underwent FNAC during 1 year from January 2017 to December 2017 were included in the study. The slides along with records of the patients were retrieved and findings recorded. Histopathological evaluation was performed wherever possible and compared with cytological diagnosis.

Non-neoplastic lesions accounted for 80.3% cases followed by benign tumors (15.2%). Ganglion cyst was the commonest non-neoplastic lesion (60.6%, 40/66), while tenosynovial giant cell tumor was the commonest neoplasm (12.1%, 8/66) observed in our study. Solitary case of synovial sarcoma was also observed. Histopathological evaluation was performed in 14 cases and FNAC had overall diagnostic accuracy of 85.7% in diagnosis of these lesions.

FNAC is a useful tool with high diagnostic accuracy in the evaluation of synovial lesions.
FNAC is a useful tool with high diagnostic accuracy in the evaluation of synovial lesions.
Metastasis to the breast of an extra-mammary origin is very rare. FNAC plays an important role in differentiating non-mammary breast metastasis from primary malignancy. This study aimed to analyze the cytomorphological criteria and its pitfalls in differentiating metastatic lesion of the breast from primary malignancy.

Retrospective analysis of 891 FNACs of the breast was performed for a time span of 3 years. A total of 12 cases were diagnosed on FNAC as secondary neoplasms to the breast. Clinical and radiological data, along with Pap and MGG stained smears of each case were examined and correlated with the histopathology of the primary tumor. Statistical analysis was carried out. All cases of primary breast malignancies were excluded from our study.

In 10 out of 12 cases, primary malignancies were identified as Plasma cell myeloma (one case), B-acute lymphoblastic leukemia (two cases), acute myeloid leukemia (one case); chronic myeloid leukemia (one case), Burkitt's lymphoma of the ovary (one case), Diffuse large B-cell lymphoma (one case), esophageal squamous cell carcinoma (one case), spindle cell sarcoma (one case) and squamous cell carcinoma of the cervix (one case). The remaining two cases in our study were misdiagnosed on cytology as metastasis and turned out to be breast primaries on histopathology.

Our case series highlights the importance of FNAC to differentiate secondary lesions from primary breast malignancy and thus helps to avoid unnecessary surgery to the patient. It emphasizes on the need to keep in mind the possibility of metastatic breast neoplasms in the presence of unusual cytological features on FNAC.
Our case series highlights the importance of FNAC to differentiate secondary lesions from primary breast malignancy and thus helps to avoid unnecessary surgery to the patient. It emphasizes on the need to keep in mind the possibility of metastatic breast neoplasms in the presence of unusual cytological features on FNAC.Fire hazards and infectious diseases result in great threats to public safety and human health, thus developing multi-functional materials to deal with these issues is critical and yet has remained challenging to date. In this work, we report a facile and eco-friendly synthetic approach for the preparation of calcium alginate/silver chloride (CA/AgCl) nanocomposites with dual functions of excellent flame-retardant and antibacterial activity. Multi characterization techniques and antibacterial assays were performed to investigate the flame-retardant and antibacterial properties of the CA/AgCl nanocomposites. The obtained results show that the CA/AgCl nanocomposites exhibited much higher limiting oxygen index value (> 60%) than that of CA (48%) with a UL-94 rating of V-0. Moreover, CA/AgCl particularly displayed an efficiently smoke-suppressive feature by achieving a total smoke release value of 2.7 m2/m2, which was reduced by 91%, compared to CA. The antibacterial rates of the CA/AgCl nanocomposites against E. coli and S. aureus were measured to be 99.67% and 99.77%, respectively, while CA showed quite weak antibacterial rates. In addition, the flame-retardant and antibacterial mechanisms were analyzed and proposed based on the experimental data. This study provides a novel nanocomposite material with both flame-retardant and antibacterial properties which show promising application prospects in the fields of decorative materials and textile industry.COVID-19 is one of the most impactful pandemics in recent history, not only in terms of direct casualties but also regarding socio-economic impact. The goal of our study is to investigate the degree of synchronization between the number of confirmed cases in specific countries, on one hand, and how/at which stage these countries adapted their air transportation operations, on the other hand. We investigate the global air transportation system as a network of countries whose edges represent the existence of direct flights. Aggregated analysis of this country network and its evolving dynamics leads to novel insights regarding the synchronization with the number of confirmed cases; finding that most country borders were likely closed too late. We believe and hope that our analysis leads to a more efficient/effective prevention and control of future epidemics.Covid-19 seriously impacts and endangers lives of millions worldwide. To fight the spread of the virus, governments have taken various restricting measures including stay at home orders. Ultimately, the home delivery volume increased significantly, which still bears the risk of human-human infection during the final delivery. From a logisticians perspective, autonomous delivery vehicles (ADVs), which are a contactless delivery solution, have the potential to radically change the way groceries are delivered to customer homes and help to stop the spread of the virus. However, to date, research on user acceptance of ADVs is rare. This paper theoretically extends the Unified Theory of Acceptance and Use of Technology (UTAUT2) including gender as a moderator. The study is based on quantitative data collected in Germany through an online questionnaire (n = 501). Data were analysed using structural equation modelling. The results indicate that trust in technology, price sensitivity, innovativeness, performance expectancy, hedonic motivation, social influence, and perceived risk determine behavioural intention. However, some constructs are only significant for women. The findings of this paper have theoretical, managerial and policy contributions and implications within the areas of last-mile delivery and technology acceptance.Optimal economic evaluation is pivotal in prioritising the implementation of non-pharmaceutical and pharmaceutical interventions in the control of diseases. Governments, decision-makers and policy-makers broadly need information about the effectiveness of a control intervention concerning its cost-benefit to evaluate whether a control intervention offers the best value for money. The outbreak of COVID-19 in December 2019, and the eventual spread to other parts of the world, have pushed governments and health authorities to take drastic socioeconomic, sociocultural and sociopolitical measures to curb the spread of the virus, SARS-CoV-2. To help policy-makers, health authorities and governments, we propose a Susceptible, Exposed, Asymptomatic, Quarantined asymptomatic, Severely infected, Hospitalized, Recovered, Recovered asymptomatic, Deceased, and Protective susceptible (individuals who observe health protocols) compartmental structure to describe the dynamics of COVID-19. We fit the model to real data from G, but is economically expensive. In case only one control is affordable, then transmission reduction is better than case isolation. Hopefully, the results of this research should help policy-makers when dealing with multiple waves of COVID-19.In this paper, we investigate an epidemic model of the novel coronavirus disease or COVID-19 using the Caputo-Fabrizio derivative. We discuss the existence and uniqueness of solution for the model under consideration, by using the the Picard-Lindelöf theorem. Further, using an efficient numerical approach we present an iterative scheme for the solutions of proposed fractional model. Finally, many numerical simulations are presented for various values of the fractional order to demonstrate the impact of some effective and commonly used interventions to mitigate this novel infection. From the simulation results we conclude that the fractional order epidemic model provides more insights about the disease dynamics.The new coronavirus disease (COVID-19), which first appeared in China in December 2019, has pervaded throughout the world. Because the epidemic started later in Turkey than other European countries, it has the least number of deaths according to the current data. Outbreak management in COVID-19 is of great importance for public safety and public health. For this reason, prediction models can decide the precautionary warning to control the spread of the disease. Therefore, this study aims to develop a forecasting model, considering statistical data for Turkey. Box-Jenkins Methods (ARIMA), Brown's Exponential Smoothing model and RNN-LSTM are employed. ARIMA was selected with the lowest AIC values (12.0342, -2.51411, 12.0253, 3.67729, -4.24405, and 3.66077) as the best fit for the number of total case, the growth rate of total cases, the number of new cases, the number of total death, the growth rate of total deaths and the number of new deaths, respectively. The forecast values of the number of each indicator are stable over time. In the near future, it will not show an increasing trend in the number of cases for Turkey. In addition, the pandemic will become a steady state and an increase in mortality rates will not be expected between 17-31 May. ARIMA models can be used in fresh outbreak situations to ensure health and safety. It is vital to make quick and accurate decisions on the precautions for epidemic preparedness and management, so corrective and preventive actions can be updated considering obtained values.
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