Notes

Serum circulating miRNA-342-3p as being a prospective analytical biomarker inside parathyroid carcinomas: An airplane pilot study.
Four of 6 patients (66%) who underwent SS-AVS were diagnosed with unilateral disease (median LI = 43.3; range 23.9-54.8), with one patient's diagnosis reliant upon a single super-selective sample. In total, 9 patients underwent adrenalectomy after repeat AVS, all of whom had improved blood pressure control postoperatively. Ten patients did not undergo repeat AVS 6 were lost to follow-up, 3 underwent medical management, and 1 underwent adrenalectomy.

AVS should be repeated when "double-down" ABAS results are encountered. Super-selective sampling may provide worthwhile diagnostic data when employed during repeat AVS.
AVS should be repeated when "double-down" ABAS results are encountered. Super-selective sampling may provide worthwhile diagnostic data when employed during repeat AVS.
To evaluate the safety, efficacy and cost of paravertebral block anesthesia for ureteral stones patients undergoing ureteroscopic lithotripsy.

Four hundred and eighty-two patients who underwent ureteroscopy for unilateral ureteral stones were incorporated into our retrospective study. A propensity-matched comparison in patients with paravertebral nerve block anesthesia (PVB) group and general anesthesia (GA) group was performed. Intraoperative hemodynamic parameters, operative time, visual analog scale for pain, stone-free rate, anesthetic cost and postoperative hospital stay were compared between the two groups.

Sixty-one GA cases were propensity matched to 61 PVB cases. In the PVB group, all the procedures were completed successfully without anesthesia conversion. Significantly less intraoperative severe hypotensive (P=0.002) and arrhythmia (P<0.001) episodes in PVB group. There were no significant differences in operative time (p=0.702), initial stone-free rate (p=0.686), and total stone-free rate (p=0.794) between the two groups. The PVB group had lower postoperative pain and prolonged analgesia (p=0.007). The postoperative hospital stay in the PVB group was significantly shorter (3.20±0.73 vs 3.84±1.32d, p=0.001). And the cost of anesthesia was lower in the PVB group (195.47±13.01 vs 396.31±36.45 US dollars, p<0.001).

Under PVB anesthesia, URS can be successfully completed without anesthetic transformation, and its efficacy and safety have been demonstrated. When economic aspects are taken into consideration, PVB seems to be a more economical and effective anesthetic method of URS.
Under PVB anesthesia, URS can be successfully completed without anesthetic transformation, and its efficacy and safety have been demonstrated. When economic aspects are taken into consideration, PVB seems to be a more economical and effective anesthetic method of URS.
The aim of this study was to assess the safety of several modified Nuss procedures for severe pectus excavatum (PE).

Thirty-four patients with severe PE underwent the Nuss procedure 10 underwent slanting-directed bar insertion (group A); 11 underwent standard Nuss procedure (group B); and 13 underwent Nuss procedure with subxiphoid assistance (group C). All the patients met the criteria of having a Haller index greater than 4.5, assessed from chest computed tomography. Besides, the transverse length of the most depressed point and the 2-intercostal left slant length between the heart and the anterior chest wall were measured.

All patients were followed up for 6-45 months (mean 31.4±11.38 months). None of the patients suffered from injuries to the pericardium, heart or lungs. There were no significant differences in age, Haller's index, operation time and postoperative stay among the three groups. However, two patients in group B experienced bar rotation and subsequently required reoperation vs the other two groups (p<0.001). The length of contact between the heart and the chest wall was shorter in the left slant axis (5.8±0.33cm) than in the transverse axis (7.3±0.37cm) in group A (p=0.001).

A modified Nuss procedure of slanting steel bar insertion has shown to be a safe and effective approach for the correction of severe PE.
A modified Nuss procedure of slanting steel bar insertion has shown to be a safe and effective approach for the correction of severe PE.
The Scoreflex NC scoring angioplasty catheter is designed with a short rapid-exchange tip distal to a non-compliant, high-pressure balloon and an integral wire outside of the balloon, such that the guidewire and the integral wire act as scoring elements during balloon inflation. The external scoring elements enable a focal stress pattern facilitating expansion of resistant lesions at lower pressures using a focused force angioplasty effect.

Patients undergoing elective percutaneous coronary intervention (PCI) were enrolled in a prospective, single-arm study conducted at 12 centers in the United States. The primary endpoint was device procedural success, defined as the composite of successful device delivery to the target lesion with balloon inflation and deflation; absence of vessel perforation, flow-limiting dissection or reduction in TIMI flow from baseline; and achievement of final TIMI 3 flow.

Among 200 patients (234 lesions), lesion complexities included bifurcation disease (37.6%), moderate/severe calcification (36.6%), and total occlusions (5.0%). Successful delivery to the target lesion, inflation and removal of the balloon catheter was achieved in 95.5% of patients (191/200). Procedural success was achieved in 93.5% (187/200) of patients, and final TIMI 3 flow was observed in 99.0% of cases (198/200). No unanticipated device-related events occurred. In-hospital major adverse events were reported in 4.5% of patients (9/200), related to periprocedural myocardial infarction (8/200, 4.0%) and target lesion revascularization (1/200, 0.5%).

Among patients undergoing elective PCI and with varied lesion complexity, these results support the safety and effectiveness of a dilation strategy using the Scoreflex NC scoring catheter.
Among patients undergoing elective PCI and with varied lesion complexity, these results support the safety and effectiveness of a dilation strategy using the Scoreflex NC scoring catheter.Linoleic acid (LA) has garnered much attention due to its potential applications in the oleochemical and nutraceutical industries. The oleaginous yeast Rhodotorula toruloides has outstanding lipogenecity, and is considered a potential alternative to the current plant-based platforms for LA production. Δ12-fatty acid desaturases (Δ12-Fads) are involved in LA synthesis in various fungi and yeasts, but their functions in R. toruloides remain poorly understood. To achieve the production of LA-rich lipids in R. toruloides, we investigated the function of the native Δ12-FAD (RtFAD2). First, the overexpression of RtFAD2 and its co-overexpression with RtFAD1 (encoding R. toruloides Δ9-Fad) and their effects on LA production in R. toruloides were investigated. The function of RtFad2 was confirmed by heterologous expression in Saccharomyces cerevisiae. Overexpression of RtFAD2 significantly elevated the LA contents and titers in the wild-type strain R. toruloides DMKU3-TK16 (TK16) and in a thermotolerant derivative of TK16 (L1-1). Additionally, overexpression of RtFAD2 in R. toruloides strains also increased the lipid titer and content. Overexpression of RtFAD1 was down-regulated in the RtFAD1 and RtFAD2 co-overexpressing strains, suggesting that the elevated LA content may function as a key regulator of RtFAD1 expression to control C18 fatty-acid synthesis in R. toruloides. We characterized the function of RtFAD2 and showed that its overexpression in R. toruloides increased the lipid and LA production. selleckchem These findings may assist in the rational design of metabolic engineering related to LA or polyunsaturated fatty acid production in R. toruloides.
Diagnostic and post-induction
I-meta-iodobenzylguanidine (
I-mIBG) scans have prognostic significance in the treatment of neuroblastoma, but data from low- and middle-income countries are limited due to resource constraints. The aim of this study was to determine the association between neuroblastoma-associated tumour markers (lactate dehydrogenase [LDH], ferritin and MYCN amplification) and
I-mIBG scans (modified Curie scores and metastatic disease patterns) in predicting complete metastatic response rates (mCR) and overall survival.

Two hundred and ninety patients diagnosed with high-risk neuroblastoma in South Africa between January 2000 and May 2018 and a subanalysis of 78 patients with diagnostic
I-mIBG scans were included. Data collection included LDH, ferritin and MYCN amplification at diagnosis. Two nuclear physicians independently determined the modified Curie scores and pattern of distribution for each diagnostic and post-induction
I-mIBG scans with high inter-rater agreement (r = 0.952the modified Curie scores correlated with each other. MYCN amplification neither correlated with any aspect of the
I-mIBG scans nor significantly predicted mCR or 2-year overall survival. LDH and ferritin are therefore appropriate neuroblastoma tumour markers to be used in low- and middle-income countries with limited or no access to mIBG scans and/or MYCN amplification studies.
LDH, ferritin and the diagnostic 123I-mIBG scans significantly predicted mCR, but only LDH predicted 2-year overall survival. Ferritin and the modified Curie scores correlated with each other. MYCN amplification neither correlated with any aspect of the 123I-mIBG scans nor significantly predicted mCR or 2-year overall survival. LDH and ferritin are therefore appropriate neuroblastoma tumour markers to be used in low- and middle-income countries with limited or no access to mIBG scans and/or MYCN amplification studies.
To assess telemedicine satisfaction of patients from a primary health care center during COVID-19 pandemics. This work could bring information on its acceptance.

Transversal study with 253 individuals. A questionnaire was performed using Likert scale (1-very unsatisfied, 5-very satisfied) on patients with diabetes, hypertension and in subjects with other pathologies, whose consultations occurred between 01/04/2020 and 01/05/2020. Collected data included sociodemographics, previous telemedicine appointments, satisfaction levels of teleconsultation and doubt clarification, and interest in further telemedicine follow-up. In addition, a comparative analysis was performed between the periods January-February and April-May of 2020 in relation to age, sex, hypertension, diabetes and the type of contact (presential or remote). Statistics were performed using SPSS® and Excel®.

Diabetes follow-up appointments accounted for 34.4% of total consultations. Globally there was a high level of satisfaction and interest telemedicine in primary health care, especially during pandemics. Participants recognized the usefulness of telemedicine and allowed future similar consultations.
This study allowed us to confirm the importance of telemedicine in primary health care, especially during pandemics. Participants recognized the usefulness of telemedicine and allowed future similar consultations.Anaphase A is the motion of recently separated chromosomes to the spindle pole they face. It is accompanied by the shortening of kinetochore-attached microtubules. The requisite tubulin depolymerization may occur at kinetochores, at poles, or both, depending on the species and/or the time in mitosis. These depolymerization events are local and suggest that cells regulate microtubule dynamics in specific places, presumably by the localization of relevant enzymes and microtubule-associated proteins to specific loci, such as pericentriolar material and outer kinetochores. Motor enzymes can contribute to anaphase A, both by altering microtubule stability and by pushing or pulling microtubules through the cell. The generation of force on chromosomes requires couplings that can both withstand the considerable force that spindles can generate and simultaneously permit tubulin addition and loss. This chapter reviews literature on the molecules that regulate anaphase microtubule dynamics, couple dynamic microtubules to kinetochores and poles, and generate forces for microtubule and chromosome motion.
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